Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Brf not contaminating!
    #21856399 - 06/25/15 06:13 PM (8 years, 7 months ago)

Something is puzzling me.

I have 1/4 pint pftek jars that I birthed before full colonization.  I'm doing this as a learning exercise.


I cracked the jars and smelled the jar in the open air half a dozen times with no regard to sterility.
It's been four days.. since I opened them

I expected to see mold cobweb or otherwise or atleast smell something off.  Nothing!  The mush myc continues to grow nicely.
:crankey:
Why, why tell me why!!?

:lol:

  Does brf just take longer to go bad or what?  I pced the jars for 2 hours.

My experience with other grains is that in 3-4 days grains will get smelly and visibly moldy...  no questions asked!


Extras: Filter Print Post Top
OfflineDiddly
FBI Van
Male

Registered: 05/29/15
Posts: 11
Loc: That one place
Last seen: 8 years, 6 months
Re: Brf not contaminating! [Re: invitro]
    #21856434 - 06/25/15 06:19 PM (8 years, 7 months ago)

Im not sure what you mean by birthed them if they're still in jars. I assume you have a verm layer on top as per pf tek which acts as a great barrier against contaminants.


--------------------
You're not drunk until you hold onto the ground for support.


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Brf not contaminating! [Re: invitro]
    #21856448 - 06/25/15 06:21 PM (8 years, 7 months ago)

I mean I opened the jars to open air and smelled them and put the lid back on....  Not really birthing.

I injected through a ship, no verm barrier.... brown rice clearly visible on top layer...


Extras: Filter Print Post Top
OfflineDiddly
FBI Van
Male

Registered: 05/29/15
Posts: 11
Loc: That one place
Last seen: 8 years, 6 months
Re: Brf not contaminating! [Re: invitro]
    #21856475 - 06/25/15 06:25 PM (8 years, 7 months ago)

Then you have a very clean environment i guess. I havent done this without a verm barrier so i cant be sure about this situation


--------------------
You're not drunk until you hold onto the ground for support.


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Brf not contaminating! [Re: Diddly]
    #21856501 - 06/25/15 06:30 PM (8 years, 7 months ago)

I'm talking ground zero for trich, the front lines people, D-day! 

I inoculate soil with trich spores (for plants) and get it all over myself, almost daily...


Extras: Filter Print Post Top
InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: Brf not contaminating! [Re: invitro]
    #21856641 - 06/25/15 07:03 PM (8 years, 7 months ago)

Ime trich and other molds take about 5 days to germinate/show signs of growth, even on agar.


--------------------


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Brf not contaminating! [Re: invitro]
    #21862280 - 06/27/15 12:39 AM (8 years, 7 months ago)

Ok 1 jar contamed pretty badly today, yay!

Maybe the grains were dryish.  I cooked the pf jars for 2 hours instead of 45 min like I was supposed to.  I don't really understand how they could loose moisture with steam everywhere buy I guess they do somehow.  the others are still contam free.


Extras: Filter Print Post Top
Offlinebluegill
intergalactic toejam
 User Gallery


Registered: 11/05/13
Posts: 489
Last seen: 2 years, 11 months
Re: Brf not contaminating! [Re: invitro]
    #21862334 - 06/27/15 12:57 AM (8 years, 7 months ago)

Is it really a good idea to sniff a contam, especially if you don't know what it is?!


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Brf not contaminating! [Re: bluegill]
    #21863713 - 06/27/15 10:46 AM (8 years, 7 months ago)

Haha well unless his immune system sucks major ass, he should be fine. Just don't go sniffing fusarium, black molds, or aspergillus.

Grains and brf believe it or not are actually pretty contamination prone when comparing to LC and agar. That's why you'd see way less cube spores germinating than you would on agar. Opening an agar dish to sniff is a disaster. It wouldn't have gone as smoothly as sniffing your spawn has :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Sniffing jars rhizo 1,214 5 11/19/02 03:26 PM
by lvleph
* Contamination issues kykeon 1,159 1 06/13/02 01:31 AM
by Roger_irrelevant
* Smelt Jars for contamination. I was taken to ER!!!
( 1 2 3 4 5 6 all )
JayBin 42,892 110 01/15/21 06:15 AM
by bodhisatta
* eating from contaminated cakes... sensi 1,371 5 12/01/03 04:55 PM
by MAIA
* Help! Newby who sniffed possibly contaminated jar!!!!! SlapnutRob 1,211 1 03/31/03 01:47 PM
by MAIA
* Contamination causes.... umb_hunk 1,237 2 11/20/02 03:33 PM
by WildCardsRevenge
* Unsure with contamination problem ram2453872 1,918 6 09/29/03 12:55 PM
by ram2453872
* Contaminants Semilanceata 956 3 07/16/03 08:52 AM
by MAIA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
478 topic views. 36 members, 212 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.