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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Buck513
Registered: 04/17/14
Posts: 5,682
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Spores to lc is one of the worst methods to go about growing.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: First timer, SW PE. [Re: Buck513]
#21988388 - 07/24/15 02:33 AM (8 years, 6 months ago) |
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I was only screwing with it before I had agar. I'd rather make a muda blender jar and do LI or just use a wedge.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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2cc seems pretty standard for 250ml of liquid. I made 4 PE LCs to increase the chances of having 1 viable one. Also i have PE tubs that ill be able to clone from, 4 grain jars going of pe that i can do g2g. All i did was go through 2 PE syringes but still have many ways to continue PE. as for MS of PE, reliable vendors still carry good PE MS and can order another if i needed.
-------------------- Order me some fucking golf shoes!!!!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First timer, SW PE. [Re: lbot1983]
#21988774 - 07/24/15 07:46 AM (8 years, 6 months ago) |
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Quote:
lbot1983 said: 2cc seems pretty standard for 250ml of liquid. I made 4 PE LCs to increase the chances of having 1 viable one. Also i have PE tubs that ill be able to clone from, 4 grain jars going of pe that i can do g2g. All i did was go through 2 PE syringes but still have many ways to continue PE. as for MS of PE, reliable vendors still carry good PE MS and can order another if i needed.
If you put that much liquid in each LC, you actually decreased the chances of them being viable. By a huge margin.
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#21988781 - 07/24/15 07:49 AM (8 years, 6 months ago) |
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I plan on starting agar work soon as well, just want to ensure i have the basics locked down first. I have read many times that MS LC inoculations are a crap shoot but still a good way for a noob learn from. Im a hands on kind of guy and like to give lots of things a try. You read 10 post on how sucessfull thier ms lc was and 10 on how crappy it was, in the end all we can really do to sort through misinformation is learn first hand.
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: Inocuole]
#21988787 - 07/24/15 07:52 AM (8 years, 6 months ago) |
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Quote:
Inocuole said:
Quote:
lbot1983 said: 2cc seems pretty standard for 250ml of liquid. I made 4 PE LCs to increase the chances of having 1 viable one. Also i have PE tubs that ill be able to clone from, 4 grain jars going of pe that i can do g2g. All i did was go through 2 PE syringes but still have many ways to continue PE. as for MS of PE, reliable vendors still carry good PE MS and can order another if i needed.
If you put that much liquid in each LC, you actually decreased the chances of them being viable. By a huge margin.
Thanks, i actually never heard that yet. Most teks i read for LC were with agar or flesh. The MS syringe teks i read all said 1-4cc so i used the mean value.
-------------------- Order me some fucking golf shoes!!!!
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#21988794 - 07/24/15 07:56 AM (8 years, 6 months ago) |
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Inocuole you got 5 shrooms from me for helping sort through bs recently, thanks much.
-------------------- Order me some fucking golf shoes!!!!
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#21990386 - 07/24/15 01:59 PM (8 years, 6 months ago) |
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Day 6 in the greenhouse for the 2 cased tubs, day 4 for the uncased. The casing layer is nearly 100% colonized on both cased tubs. Lots and lots of healthy mycelium but no signs of fruiting. Based on my research i should see pins in 4-8 days. This is like waiting for christmas when i was a kid. Just glad i have so many other things that i need to do or it may be all consuming. I have 2 out buildings with climate control i may convert for various edible and medicinal mushrooms.
 How do they look?
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#21995234 - 07/25/15 01:14 PM (8 years, 6 months ago) |
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Casing layer on both cased tubs at 100%. Still no signs of knots. Dont expect any gor atleast a few more days.
-------------------- Order me some fucking golf shoes!!!!
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: First timer, SW PE. [Re: lbot1983]
#21995251 - 07/25/15 01:20 PM (8 years, 6 months ago) |
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Nice. Just be patient, they will start popping up in no time. That is a good idea for those buildings if they are climate controlled good luck with that.
Edited by insanemike (07/25/15 01:20 PM)
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Indeed they are fully climate controled. They were my old veg and flower rooms for cannabis before my county made it illegal to grow even with medical card.
Whats up with this mycelium growth?
-------------------- Order me some fucking golf shoes!!!!
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#21996432 - 07/25/15 05:46 PM (8 years, 6 months ago) |
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Im probably just being noob looking to hard trying to learn but i just want to learn what i am seeing. Got that marshmallow like growth in the last pic and now i notice this...could it be primordia?
-------------------- Order me some fucking golf shoes!!!!
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nobody83
stranger danger


Registered: 03/15/14
Posts: 1,486
Loc: around town
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Re: First timer, SW PE. [Re: lbot1983]
#21996499 - 07/25/15 05:58 PM (8 years, 6 months ago) |
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Doesn't look like it but i could be wrong
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First timer, SW PE. [Re: lbot1983]
#21996501 - 07/25/15 05:59 PM (8 years, 6 months ago) |
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Can't really tell but just chill out, it doesn't look too crazy yet. Might have a little bacteria maybe. And if your posts are so close together it's best to use the edit function.
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lbot1983
Doctor Ganja


Registered: 06/19/15
Posts: 115
Last seen: 8 years, 3 months
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Re: First timer, SW PE. [Re: Inocuole]
#22005680 - 07/27/15 05:26 PM (8 years, 6 months ago) |
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Hows things looking on this tub? I think i see knots on the casing layer. There are a shit ton of knots between the tub walls and sub.
-------------------- Order me some fucking golf shoes!!!!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First timer, SW PE. [Re: lbot1983]
#22006063 - 07/27/15 06:26 PM (8 years, 6 months ago) |
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Knots?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First timer, SW PE. [Re: taGyo]
#22006077 - 07/27/15 06:27 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Knots?
There do appear to be some hyphal knots there... If that's not a buncha knots then it's sick mycelium that looks highly similar by virtue of how colonized the casing layer is.
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Buck513
Registered: 04/17/14
Posts: 5,682
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Re: First timer, SW PE. [Re: taGyo]
#22006086 - 07/27/15 06:28 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Knots?
noun plural noun: knots 1. a fastening made by tying a piece of string, rope, or something similar. "tie a knot at the end of the cord" synonyms: tie, twist, loop, bow, hitch, half hitch, clove hitch, join, fastening; More a particular method of tying a knot. "you need to master two knots, the clove hitch and the sheet bend" a tied or folded ribbon, worn as an ornament. 2. a tangled mass in something such as hair. 3. a knob, protuberance, or node in a stem, branch, or root. synonyms: nodule, gnarl, node; More a hard mass formed in a tree trunk at the intersection with a branch, resulting in a round cross-grained piece in timber when cut through. a hard lump of tissue in an animal or human body. 4. an unpleasant feeling of tightness or tension in a part of the body. "her stomach was in knots as she unlocked the door" 5. a small tightly packed group of people. "the little knot of people clustered around the doorway" 6. a unit of speed equivalent to one nautical mile per hour, used especially of ships, aircraft, and winds. historical a length marked by knots on a log line, as a measure of speed. "some days the vessel logged 12 knots" verb 3rd person present: knots 1. fasten with a knot. "the scarves were knotted loosely around their throats" synonyms: tie (up), fasten, secure, bind, do up "a long blue scarf was knotted around her waist" make (a carpet or other decorative item) with knots. 2. make (something, especially hair) tangled. synonyms: tangled, tangly, knotty, entangled, matted, snarled, unkempt, uncombed, tousled; informalmussed up "knotted hair"
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First timer, SW PE. [Re: Inocuole]
#22006092 - 07/27/15 06:30 PM (8 years, 6 months ago) |
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Quote:
Inocuole said:
Quote:
taGyo said: Knots?
There do appear to be some hyphal knots there... If that's not a buncha knots then it's sick mycelium that looks highly similar by virtue of how colonized the casing layer is.
I might see one or two, my casing layers never look like that I guess.
@Buck
Thanks for the dictionary.com , I was implying I saw a colonizing casing layer and maybe a few tiny buttons . Maybe it's just the pic.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
Edited by taGyo (07/27/15 06:30 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: First timer, SW PE. [Re: taGyo]
#22006117 - 07/27/15 06:31 PM (8 years, 6 months ago) |
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Eh I only see maybe 6-7 of them without looking too hard... they're not exactly everywhere. A better pic would say more than I can.
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