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Offlinelbot1983
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Registered: 06/19/15
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First timer, SW PE.
    #21851656 - 06/24/15 05:42 PM (8 years, 7 months ago)

Hello shroomery and welcome to my first journey into mycology. I spent many many hours on here reading posts from the best of you, not gonna use names to keep from leaving any props out, so props to all.

Now onto my grow. Pf tek brf jars sterilized on the 15th, 9 days later lots of growth on all 9 jars. I read PE is a slow colonizer and fruiter but also read about using a little more then 1cc per jar to speed it up but not add to much extra moisture. I used 1.5cc per jar. Seems like the mycelium is pretty vigours for PE but read more then once that SW PE is the best in the buisness. I plan to spawn to bulk hpoo tek as i have horses. 6qt dub tubs is my FC.

I got 2 PE MS from SW, used 1 for the 9 jars and the other for 4 LCs. The LCs were inoculated on the 18th, all have mycelium growing, hopeing for atleast on viable culutre but hooefully all 4. I was very clean and anal about sterilization. I know my home is clean of mold as it is a more recent home in a dry desert, mold her is obvious and confined to bathrooms and even then in rare in homes under 25 years old.

I hope all goes well, wish me luck in my journel. Also, the fruit will be used to study psilocybin and ptsd by micro dosing cubensis.

Enjoy the pics of my mycelium as of today. I will update daily if not every other day.







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InvisiblebodhisattaMDiscordReddit
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Re: First timer, SW PE. [Re: lbot1983]
    #21851710 - 06/24/15 05:55 PM (8 years, 7 months ago)

great job, next time though you can use even less than 1cc, even just a drop per hole is fine. the reason there's 4 holes on a PF tek is to get things spread around and faster colonization.
A single drop of spore solution has over 1000s of spores and you only need 2 compatible ones. check out the very last link in my signature.

PE they say this and that but it will grow, patience is the biggest part.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: First timer, SW PE. [Re: bodhisatta]
    #21851727 - 06/24/15 05:58 PM (8 years, 7 months ago)

I doubt your home is mold free.

Don't use that as an excuse to get sloppy


--------------------
Fail to plan and you plan to fail.

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InvisiblebodhisattaMDiscordReddit
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Re: First timer, SW PE. [Re: Buck513]
    #21851741 - 06/24/15 06:01 PM (8 years, 7 months ago)

yep even in your "mold free house" every single breath you take has 100s of spores. you can't sanitize a residential room let alone sterilize one.


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Offlinelbot1983
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Re: First timer, SW PE. [Re: bodhisatta]
    #21852108 - 06/24/15 07:28 PM (8 years, 7 months ago)

Never once did i say i was sloppy, i was just saying that i was anal about everything and there is no mold blooms growing in my house...i have hepa air filter and uv air filter in my house. As per a very reconized cultivator posting that continual failure is usally a sign of mold growing in the house. I was trying to imply that if shit fucks up i fucked it up and its not the enviornment. Why do people seem to read things that were in no way implied, what so ever.


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OfflinePussyFart
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Re: First timer, SW PE. [Re: lbot1983]
    #21852122 - 06/24/15 07:32 PM (8 years, 7 months ago)

Quote:

lbot1983 said:
As per a very reconized cultivator posting that continual failure is usally a sign of mold growing in the house.



Yea, blame it on the mold spore count in the air, not the sterile technique of the user......lol.

You could put me, a SAB, and 100 jars in a room full of trich....I will do 100 G2G transfers and still come out a winner....

You control the failure rate, not the mold spore count.


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InvisibleSteveRogers
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Re: First timer, SW PE. [Re: PussyFart]
    #21852137 - 06/24/15 07:36 PM (8 years, 7 months ago)

Amen PussyFart.

To the poster. Your doing well man. Growth in 9/9 jars first time and LC's rollin is a healthy start. Well played.


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"General, I am loyal to nothing......except The Dream"


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Offlinelbot1983
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Re: First timer, SW PE. [Re: lbot1983]
    #21852138 - 06/24/15 07:37 PM (8 years, 7 months ago)

I was not implying that there are no spores in my home or bacteria in my breath, just again just saying, the dry climate i live in is very unhospital to mold spores germinating. I know the risks, i was anal, surgical mask, gloves, flow hood, 91% iso, alcohol wipes, 24 hour cooling before opening PC and alcohol lamp for flaming the needle before each jar. I took every precaution. Nothing sloppy about my sterilization process. I dont even move the jars, pics are taken in place. Im very anal. But i do appreciate the looking out from you guys.


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InvisibleSteveRogers
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Re: First timer, SW PE. [Re: lbot1983]
    #21852145 - 06/24/15 07:39 PM (8 years, 7 months ago)

even when its dry, anal is good. (climate joke).


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"General, I am loyal to nothing......except The Dream"


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Offlinelbot1983
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Re: First timer, SW PE. [Re: PussyFart]
    #21852147 - 06/24/15 07:39 PM (8 years, 7 months ago)

Quote:

PussyFart said:
Quote:

lbot1983 said:
As per a very reconized cultivator posting that continual failure is usally a sign of mold growing in the house.



Yea, blame it on the mold spore count in the air, not the sterile technique of the user......lol.

You could put me, a SAB, and 100 jars in a room full of trich....I will do 100 G2G transfers and still come out a winner....

You control the failure rate, not the mold spore count.



Blame what, i said nothing of contam in my jars and clearly said if anything goes wrong I FUCKED IT UP.


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InvisiblebodhisattaMDiscordReddit
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Re: First timer, SW PE. [Re: lbot1983]
    #21852153 - 06/24/15 07:40 PM (8 years, 7 months ago)

Quote:

lbot1983 said:
I was not implying that there are no spores in my home or bacteria in my breath, just again just saying, the dry climate i live in is very unhospital to mold spores germinating. I know the risks, i was anal, surgical mask, gloves, flow hood, 91% iso, alcohol wipes, 24 hour cooling before opening PC and alcohol lamp for flaming the needle before each jar. I took every precaution. Nothing sloppy about my sterilization process. I dont even move the jars, pics are taken in place. Im very anal. But i do appreciate the looking out from you guys.



they germinate in the jars anyway moot point
91% iso is inferior to 70% use 70% next time. it denatures proteins water is miscable with alcohol and helps it penetrate cell walls.

sloppy is not knowing about sterile process. that's the basics.


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Offlinelbot1983
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Re: First timer, SW PE. [Re: bodhisatta]
    #21852308 - 06/24/15 08:15 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
Quote:

lbot1983 said:
I was not implying that there are no spores in my home or bacteria in my breath, just again just saying, the dry climate i live in is very unhospital to mold spores germinating. I know the risks, i was anal, surgical mask, gloves, flow hood, 91% iso, alcohol wipes, 24 hour cooling before opening PC and alcohol lamp for flaming the needle before each jar. I took every precaution. Nothing sloppy about my sterilization process. I dont even move the jars, pics are taken in place. Im very anal. But i do appreciate the looking out from you guys.



they germinate in the jars anyway moot point
91% iso is inferior to 70% use 70% next time. it denatures proteins water is miscable with alcohol and helps it penetrate cell walls.

sloppy is not knowing about sterile process. that's the basics.



Quote:

bodhisatta said:
Quote:

lbot1983 said:
I was not implying that there are no spores in my home or bacteria in my breath, just again just saying, the dry climate i live in is very unhospital to mold spores germinating. I know the risks, i was anal, surgical mask, gloves, flow hood, 91% iso, alcohol wipes, 24 hour cooling before opening PC and alcohol lamp for flaming the needle before each jar. I took every precaution. Nothing sloppy about my sterilization process. I dont even move the jars, pics are taken in place. Im very anal. But i do appreciate the looking out from you guys.



they germinate in the jars anyway moot point
91% iso is inferior to 70% use 70% next time. it denatures proteins water is miscable with alcohol and helps it penetrate cell walls.

sloppy is not knowing about sterile process. that's the basics.



I used the 91% to clean my work after cleaned what i cleaned. I used wipes on the needle, jars, and anything else once the jars came out of the PC. I read something about what you have stated but had 91 on hand and figured it was more then ok for cleaning a work area. Thanks for the info, im all ears for good info.


--------------------
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InvisiblebodhisattaMDiscordReddit
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Re: First timer, SW PE. [Re: lbot1983]
    #21852341 - 06/24/15 08:21 PM (8 years, 7 months ago)

C1V1=C2V2 is the concentration calculation you can dilute it to make 70% with distilled water

(0.70)(650mL)=(.91)(500mL)

add 150ml of water to 500ml of 91% yields you 650mL of 70%.


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Offlinelbot1983
Doctor Ganja
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Registered: 06/19/15
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Re: First timer, SW PE. [Re: bodhisatta]
    #21852607 - 06/24/15 09:07 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
C1V1=C2V2 is the concentration calculation you can dilute it to make 70% with distilled water

(0.70)(650mL)=(.91)(500mL)

add 150ml of water to 500ml of 91% yields you 650mL of 70%.



Quote:

bodhisatta said:
C1V1=C2V2 is the concentration calculation you can dilute it to make 70% with distilled water

(0.70)(650mL)=(.91)(500mL)

add 150ml of water to 500ml of 91% yields you 650mL of 70%.



Thanks a bunch, i have a bunch of 91%. I always have it for cleaning my various dabbing rigs.


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Offlinelbot1983
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Re: First timer, SW PE. [Re: lbot1983]
    #21863740 - 06/27/15 10:52 AM (8 years, 7 months ago)

Quick update, all jars have very nice mycelium growth. Ill post a pic of 3 jars over a 72 hour period.



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Offlinelbot1983
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Re: First timer, SW PE. [Re: lbot1983]
    #21889082 - 07/02/15 05:05 PM (8 years, 6 months ago)

Most jars are about 90% colonized. I got a mini greenhouse martha setup. Sealed the bottom and added a few inches of perlite. Cut some slits to provide FEA and a cool mist humidifer. Empty i can maintain 90% or greater humidity and temps of 71-76. Should be spawing to bulk coir/coffee/gypsum next week. I have enough spawn for 2 6qt shoe boxs tubs and my lc is about ready to inoculate some wbs jars. I can get 12 6 qt shoe box tubs in there.



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OfflineZeroBoyWD
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Re: First timer, SW PE. [Re: lbot1983]
    #21889620 - 07/02/15 07:21 PM (8 years, 6 months ago)

Damn. You are putting my first grow to shame ._.

Quick question. How fine did you grind up your brown rice? It looks like you broke more into pieces than powder. The reason I ask is because when I used pint jars (And I've seen others with this problem) I got mine down to a more powdery consistency and they had a tendency to stall out or separate from the rest of the substrate.


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I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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Offlinelbot1983
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Re: First timer, SW PE. [Re: ZeroBoyWD]
    #21889776 - 07/02/15 08:01 PM (8 years, 6 months ago)

Its organic brf, very fine. Im using half pint jars and did not pack them. There are nice air pockets that have colonized nicely. The first few jars are just about ready, within the week. Its been 2 weeks since inoculation with MS and from what i have read that is a pretty quick colinization from ms for PE.

Im one of those read for a year then go for it. I made 4 LCs to inoculate WBS jars shortly and realized a martha woukd be better suited for 6 qt bulk trays then having to deal with 14 dub tubs.


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OfflineZeroBoyWD
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Re: First timer, SW PE. [Re: lbot1983]
    #21889829 - 07/02/15 08:13 PM (8 years, 6 months ago)

I see, didn't have anything for scale, thought they were whole pint. You got some good technique mixing with the verm there, sir. Keep it up, I am both very impressed and very envious =)


--------------------
I'm done trying to be "right." I want the exchange of ideas to be the currency of my economy. If you have something I can use, great. I'll try it out. If you think my methods are shit, great. Don't use it. We all want yields.



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Offlinelbot1983
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Re: First timer, SW PE. [Re: ZeroBoyWD]
    #21889872 - 07/02/15 08:22 PM (8 years, 6 months ago)

Quote:

ZeroBoyWD said:
I see, didn't have anything for scale, thought they were whole pint. You got some good technique mixing with the verm there, sir. Keep it up, I am both very impressed and very envious =)



Thanks much, i have years of experiance with cannabis, from soil to auqaponics and everything in between. Most of it is useless when it comes to mushrooms but there are a few things that gave me a head start. Here's to keeping it clean.


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