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Invisible2shoes
The anti-agar
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Registered: 04/07/11
Posts: 3,124
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Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #21850765 - 06/24/15 02:36 PM (8 years, 10 months ago)

It cost less than a buck so its whatever really. Would you suggest I do it 1:1 replacement of potato flakes on a PDA recipe?

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InvisibleThoky.ykoht
Registered: 10/08/13
Posts: 399
Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #21850771 - 06/24/15 02:37 PM (8 years, 10 months ago)

Maybe I can salvage some of the healthy white from the outside but idk.

And if these plates are toast too then I'm back to taking my chances with vendors. I didn't take any clones this time around.

What about these, they've been in bags in the fridge since April.

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OfflineGreen Bastard
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Re: Post Your Cultivation Picture of The Day [Re: azur]
    #21850788 - 06/24/15 02:40 PM (8 years, 10 months ago)

Quote:

azur said:
Shredded a barrels worth of straw today





First person (second reference) here, that I've heard using a shredder. I bought a WORX shredder, a few months ago. Best investment ever!! Did 6 large 50 lb bales, in an few hours, straight into jumbo heavy grade trash bags, for storage. That, and my custom made coir grater, have shaved many many hours off of my bulk sub prep!

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InvisiblebodhisattaMDiscordReddit
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Loc: Milky way
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Re: Post Your Cultivation Picture of The Day [Re: 2shoes]
    #21850800 - 06/24/15 02:43 PM (8 years, 10 months ago)

30 g potato starch, soluble
20 g dextrose/glucose
15 g agar, pure (omit for liquid media)
1000 mL h2o.
sterilize by autoclaving at 15 pounds per square inch (100 kPa) for 15 minutes
Dispense 20-25 ml portions into sterile 15 × 100 mm petri dishes.
Adjust pH 5.6 after autoclaving

that's the potato starch agar recipe. :thumbup:

you can use up to 20g/l agar.

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OfflineGreen Bastard
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Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #21850853 - 06/24/15 02:51 PM (8 years, 10 months ago)

Quote:

bodhisatta said:
30 g potato starch, soluble
20 g dextrose/glucose
15 g agar, pure (omit for liquid media)
1000 mL h2o.
sterilize by autoclaving at 15 pounds per square inch (100 kPa) for 15 minutes
Dispense 20-25 ml portions into sterile 15 × 100 mm petri dishes.
Adjust pH 5.6 after autoclaving

that's the potato starch agar recipe. :thumbup:

you can use up to 20g/l agar.




So far, I've only used a premixed LME/Agar, but am intrigued. Is there any advantage to the potatoe starch?

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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
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Re: Post Your Cultivation Picture of The Day [Re: Green Bastard]
    #21850893 - 06/24/15 02:58 PM (8 years, 10 months ago)

Its good to rotate media, especially for long term culture storage.

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Invisiblehamloaf
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Registered: 12/23/09
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Re: Post Your Cultivation Picture of The Day [Re: azur]
    #21850942 - 06/24/15 03:07 PM (8 years, 10 months ago)

Quote:

azur said:
Shredded a barrels worth of straw today




Very cool, azur.  Oh the possibilities.  Do want.  Right now manure is shredded using a grey storage tote with 6 inch hole cut into center of lid, and an electric weed eater.  WOrks great, but takes a cool minute to get manure shredded.  Still better than doing it by hand, imho. Woud really like to utilize one of these shredders in the maner that you have (for shredding straw and manure).  What kind of shredder is that?  All that can be seen from here are the letters "MTD/O" on it??  Also, have you ever ran field-aged cow manure through that shredder?  Can't imagine the cow shit causing any sort of issue for a freakin shredder.  Stranger things have happened though.

Edited by hamloaf (06/25/15 05:01 AM)

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OfflineGreen Bastard
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Re: Post Your Cultivation Picture of The Day [Re: hamloaf]
    #21850974 - 06/24/15 03:14 PM (8 years, 10 months ago)

Quote:

Pastywhyte said:
Its good to rotate media, especially for long term culture storage.




Thank you.
Still learning the joy of agar. On some 5th transfers now. Thinking of throwing a bunch of them onto slants soon.


Quote:

hamloaf said:
Quote:

azur said:
Shredded a barrels worth of straw today




Very cool, man.  Oh the possibilities.  What kind of shredder is that?  All that can be seen from here are the letters "MTD/O" on it??




Check out a WORX leaf shredder. Essentially a weed eater, upside down, in a hopper, with a frame to hold any sized catch bag. Under $150, light, quitet, easy to clean. Super effective! Did 300 pounds of straw, in a few hours...

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InvisiblebodhisattaMDiscordReddit
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Re: Post Your Cultivation Picture of The Day [Re: Green Bastard]
    #21850982 - 06/24/15 03:16 PM (8 years, 10 months ago)

never used potato starch but it seems to be tried and true to me.

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Post Your Cultivation Picture of The Day [Re: Green Bastard]
    #21851009 - 06/24/15 03:21 PM (8 years, 10 months ago)

Imagine if you only ate cheeseburgers everyday for a week.
Then, next week, I give you more cheeseburgers.

You'd get tired of eating the same stuff wouldn't you?
So does mycelium on agar, it knows its being fed the same food.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery

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OfflinePhosCap
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Re: Post Your Cultivation Picture of The Day [Re: azur]
    #21851089 - 06/24/15 03:41 PM (8 years, 10 months ago)

Quote:

azur said:
Shredded a barrels worth of straw today





looks perfect.

Not sure if it was you or not, but a few years ago someone just ran over straw using a lawn mower or mulcher.

Do you know if its possible to put straw into a tub and weed wack it?


--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep

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Invisibled0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
Re: Post Your Cultivation Picture of The Day [Re: PhosCap]
    #21851096 - 06/24/15 03:42 PM (8 years, 10 months ago)

Pretty sure RR puts in a tub and weed whacks in his series.


--------------------

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InvisiblebodhisattaMDiscordReddit
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
    #21851151 - 06/24/15 03:53 PM (8 years, 10 months ago)

Quote:

Buck513 said:
Imagine if you only ate cheeseburgers everyday for a week.
Then, next week, I give you more cheeseburgers.

You'd get tired of eating the same stuff wouldn't you?
So does mycelium on agar, it knows its being fed the same food.



it's actually more of a stress response and similar things to "crabtree effect" that happens in yeast.

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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,227
Loc: Flag
Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #21851189 - 06/24/15 04:01 PM (8 years, 10 months ago)

:takingnotes:


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:

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InvisibleThoky.ykoht
Registered: 10/08/13
Posts: 399
Re: Post Your Cultivation Picture of The Day [Re: Fantastic Mr. Fox]
    #21851220 - 06/24/15 04:05 PM (8 years, 10 months ago)

Anyone?

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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,227
Loc: Flag
Re: Post Your Cultivation Picture of The Day [Re: Thoky.ykoht]
    #21851236 - 06/24/15 04:09 PM (8 years, 10 months ago)

Those look a bit dry aswell,
You could try transferring on to soft agar.


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:

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Invisibleeatyualive
Eat's You Alive :)
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Posts: 19,026
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Re: Post Your Cultivation Picture of The Day [Re: azur]
    #21851455 - 06/24/15 04:59 PM (8 years, 10 months ago)

Quote:

azur said:
Shredded a barrels worth of straw today




looks perfect.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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InvisiblebodhisattaMDiscordReddit
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Re: Post Your Cultivation Picture of The Day [Re: eatyualive]
    #21851638 - 06/24/15 05:38 PM (8 years, 10 months ago)

different pathways activate in fungal species in response to environment down regulation of some things and up regulation of others can lead to lags in performance

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Post Your Cultivation Picture of The Day [Re: bodhisatta]
    #21851711 - 06/24/15 05:55 PM (8 years, 10 months ago)

Bod why don't you make yourself useful and look at some APE spores under that scope and share


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery

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InvisiblebodhisattaMDiscordReddit
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Posts: 61,891
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Re: Post Your Cultivation Picture of The Day [Re: Buck513]
    #21851718 - 06/24/15 05:57 PM (8 years, 10 months ago)

make yourself useful and do something other than shit post :lol:

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Shop: Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Grow Bags   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate


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