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99.99
Stranger


Registered: 12/22/14
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This penis envy is just about ready( pure isolate ??)
#21846889 - 06/23/15 05:35 PM (8 years, 7 months ago) |
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I've been using my new flowhood and have got my cultures down to about a transfer away from a good looking isolate
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,234
Loc: Middle Earth
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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
#21846899 - 06/23/15 05:39 PM (8 years, 7 months ago) |
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Sweet...nice growth you got going on. Good luck with that.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: This penis envy is just about ready( pure isolate ??) [Re: blazedup]
#21846908 - 06/23/15 05:43 PM (8 years, 7 months ago) |
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How many transfers away from germination is that?
How many sectors did you isolate/how many isolates do you hope to end up to test?
Nice work! PE too, sweet!
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (06/23/15 05:50 PM)
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99.99
Stranger


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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
#21847046 - 06/23/15 06:20 PM (8 years, 7 months ago) |
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Quote:
mushpunx said: How many transfers away from germination is that?
How many sectors did you isolate/how many isolates do you hope to end up to test?
Nice work! PE too, sweet!
I'd say next agar plate should be ready
I've done 8 dishes of agar 3 times from MS
As far as I can tell everything from 7 o'clock till about 2 o'clock is an isolate
Edited by 99.99 (06/23/15 06:31 PM)
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mushpunx
Fungus Punk



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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
#21847109 - 06/23/15 06:36 PM (8 years, 7 months ago) |
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Wait are you saying thats only 3 transfers away from spore?
Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out
So isolate from each of the sectors, not just one
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 Amateur Mycologists United AMU Q&A
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
#21847137 - 06/23/15 06:42 PM (8 years, 7 months ago) |
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That's still gonna sector. Also start using petris so you can look at them from the bottom easier. It's easier to pick out sectors that way sometimes.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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99.99
Stranger


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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
#21847150 - 06/23/15 06:45 PM (8 years, 7 months ago) |
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Quote:
mushpunx said: Wait are you saying thats only 3 transfers away from spore?
Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out
So isolate from each of the sectors, not just one
Yes three times but the best out of 8 dishes
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
#21847183 - 06/23/15 06:55 PM (8 years, 7 months ago) |
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Quote:
99.99 said:
Quote:
mushpunx said: Wait are you saying thats only 3 transfers away from spore?
Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out
So isolate from each of the sectors, not just one
Yes three times but the best out of 8 dishes
Oh yea dude if thats only 3 transfers away from spore gernination you likely have a ways to go before you see prominent sectoring dude. Usually takes 8-10 transfers before I see sectoring.
Keep taking transfers and once you see obvious sectors isolate each of them out, so that you end up with several isolates to test out
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 Amateur Mycologists United AMU Q&A
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
#21847276 - 06/23/15 07:15 PM (8 years, 7 months ago) |
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If using 100mm plates and if you take small pieces on half strength agar you can get sectoring on the 2-3 plate.
Make sure to start by streaking your spore plates though.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Re: This penis envy is just about ready( pure isolate ??) [Re: maddchef]
#21847293 - 06/23/15 07:21 PM (8 years, 7 months ago) |
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Quote:
maddchef said: If using 100mm plates and if you take small pieces on half strength agar you can get sectoring on the 2-3 plate.
Make sure to start by streaking your spore plates though.
Really? Never happens for me man even with low nutes not 2-3. Maybe 5th or 6th plate
Although Ive just started streaking. Before I would always swipe spores up with loop and rub in a tiny circle on bank plate
--------------------
 Amateur Mycologists United AMU Q&A
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