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Offline99.99
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This penis envy is just about ready( pure isolate ??)
    #21846889 - 06/23/15 05:35 PM (8 years, 7 months ago)

I've been using my new flowhood and have got my cultures down to about a transfer away from a good looking isolate



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Offlineblazedup
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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
    #21846899 - 06/23/15 05:39 PM (8 years, 7 months ago)

Sweet...nice growth you got going on. Good luck with that.


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Offlinemushpunx
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Re: This penis envy is just about ready( pure isolate ??) [Re: blazedup]
    #21846908 - 06/23/15 05:43 PM (8 years, 7 months ago)

How many transfers away from germination is that?

How many sectors did you isolate/how many isolates do you hope to end up to test?


Nice work! PE too, sweet!


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Edited by mushpunx (06/23/15 05:50 PM)


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Offline99.99
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
    #21847046 - 06/23/15 06:20 PM (8 years, 7 months ago)

Quote:

mushpunx said:
How many transfers away from germination is that?

How many sectors did you isolate/how many isolates do you hope to end up to test?


Nice work! PE too, sweet!




I'd say next agar plate should be ready

I've done 8 dishes of agar 3 times from MS

As far as I can tell everything from 7 o'clock till about 2 o'clock is an isolate


Edited by 99.99 (06/23/15 06:31 PM)


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Offlinemushpunx
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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
    #21847109 - 06/23/15 06:36 PM (8 years, 7 months ago)

Wait are you saying thats only 3 transfers away from spore?

Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out

So isolate from each of the sectors, not just one


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Invisiblemaddchef
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
    #21847137 - 06/23/15 06:42 PM (8 years, 7 months ago)

That's still gonna sector. Also start using petris so you can look at them from the bottom easier. It's easier to pick out sectors that way sometimes.


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Offline99.99
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
    #21847150 - 06/23/15 06:45 PM (8 years, 7 months ago)

Quote:

mushpunx said:
Wait are you saying thats only 3 transfers away from spore?

Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out

So isolate from each of the sectors, not just one




Yes three times but the best out of 8 dishes


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Offlinemushpunx
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Re: This penis envy is just about ready( pure isolate ??) [Re: 99.99]
    #21847183 - 06/23/15 06:55 PM (8 years, 7 months ago)

Quote:

99.99 said:
Quote:

mushpunx said:
Wait are you saying thats only 3 transfers away from spore?

Hopefully its a good isolate! I say like 1 in 10 is usually worth keeping. Thats why I try to isolate all the visible good sectors to end up with 8-!0 isolates from a culture to test out

So isolate from each of the sectors, not just one




Yes three times but the best out of 8 dishes





Oh yea dude if thats only 3 transfers away from spore gernination you likely have a ways to go before you see prominent sectoring dude. Usually takes 8-10 transfers before I see sectoring.

Keep taking transfers and once you see obvious sectors isolate each of them out, so that you end up with several isolates to test out


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Invisiblemaddchef
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Re: This penis envy is just about ready( pure isolate ??) [Re: mushpunx]
    #21847276 - 06/23/15 07:15 PM (8 years, 7 months ago)

If using 100mm plates and if you take small pieces on half strength agar you can get sectoring on the 2-3 plate.

Make sure to start by streaking your spore plates though.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinemushpunx
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Re: This penis envy is just about ready( pure isolate ??) [Re: maddchef]
    #21847293 - 06/23/15 07:21 PM (8 years, 7 months ago)

Quote:

maddchef said:
If using 100mm plates and if you take small pieces on half strength agar you can get sectoring on the 2-3 plate.

Make sure to start by streaking your spore plates though.





Really? Never happens for me man even with low nutes not 2-3. Maybe 5th or 6th plate

Although Ive just started streaking. Before I would always swipe spores up with loop and rub in a tiny circle on bank plate


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