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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Reinnoculating jars that had no growth
    #21846158 - 06/23/15 01:50 PM (8 years, 7 months ago)

I inoculated 7 pf jars about 2 weeks ago. 2 had trich, 4 have healthy myc. growth (though only after a week and a half) and I'm currently at the 2 week mark and one of the jars shows no sign of life. If at the end of the grow this is still the case, is it fine to just reinnoc. with a new batch or will it have lost moisture or something?

Cheers


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Reinnoculating jars that had no growth [Re: nwalker248]
    #21846163 - 06/23/15 01:51 PM (8 years, 7 months ago)

Start over. If it didn't grow, something was wrong.


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bodhisatta said:
i recommend common sense and figuring it out.

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InvisibleSteveRogers
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Registered: 10/24/06
Posts: 3,450
Re: Reinnoculating jars that had no growth [Re: nwalker248]
    #21846174 - 06/23/15 01:54 PM (8 years, 7 months ago)

Throw it away and start a new jar. Someone else will break down why as far as moisture content, bacteria consuming nutrients, endospores, ect, but don't waste your syringes and time. Start a new one. Better yet start some grain jars.


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"General, I am loyal to nothing......except The Dream"


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Offlinenwalker248
Solo Trekker
I'm a teapot


Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
Re: Reinnoculating jars that had no growth [Re: SteveRogers]
    #21846455 - 06/23/15 03:28 PM (8 years, 7 months ago)

Quote:

SteveRogers said:
Throw it away and start a new jar. Someone else will break down why as far as moisture content, bacteria consuming nutrients, endospores, ect, but don't waste your syringes and time. Start a new one. Better yet start some grain jars.




I've started some WBS jars a few days ago, and will probably have them running as two seperate projects (as the 4 jars left aren't getting me very far). So come to think of it I probably won't even end up doing any more PF cakes unless I just want an easy run. Was just curious whether I could just reinnoculate it though.


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OfflineShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: Reinnoculating jars that had no growth [Re: nwalker248]
    #21850035 - 06/24/15 11:27 AM (8 years, 7 months ago)

I've reinoculated a few jars lately that for some reason stalled.  No problems but I only waited 3 weeks.


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