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nwalker248
Solo Trekker



Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
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Reinnoculating jars that had no growth
#21846158 - 06/23/15 01:50 PM (8 years, 7 months ago) |
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I inoculated 7 pf jars about 2 weeks ago. 2 had trich, 4 have healthy myc. growth (though only after a week and a half) and I'm currently at the 2 week mark and one of the jars shows no sign of life. If at the end of the grow this is still the case, is it fine to just reinnoc. with a new batch or will it have lost moisture or something?
Cheers
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Reinnoculating jars that had no growth [Re: nwalker248]
#21846163 - 06/23/15 01:51 PM (8 years, 7 months ago) |
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Start over. If it didn't grow, something was wrong.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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SteveRogers
gandy dancer


Registered: 10/24/06
Posts: 3,450
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Re: Reinnoculating jars that had no growth [Re: nwalker248]
#21846174 - 06/23/15 01:54 PM (8 years, 7 months ago) |
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Throw it away and start a new jar. Someone else will break down why as far as moisture content, bacteria consuming nutrients, endospores, ect, but don't waste your syringes and time. Start a new one. Better yet start some grain jars.
-------------------- "General, I am loyal to nothing......except The Dream"
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nwalker248
Solo Trekker



Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
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Re: Reinnoculating jars that had no growth [Re: SteveRogers]
#21846455 - 06/23/15 03:28 PM (8 years, 7 months ago) |
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Quote:
SteveRogers said: Throw it away and start a new jar. Someone else will break down why as far as moisture content, bacteria consuming nutrients, endospores, ect, but don't waste your syringes and time. Start a new one. Better yet start some grain jars.
I've started some WBS jars a few days ago, and will probably have them running as two seperate projects (as the 4 jars left aren't getting me very far). So come to think of it I probably won't even end up doing any more PF cakes unless I just want an easy run. Was just curious whether I could just reinnoculate it though.
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ShroominMe
Stranger

Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: Reinnoculating jars that had no growth [Re: nwalker248]
#21850035 - 06/24/15 11:27 AM (8 years, 7 months ago) |
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I've reinoculated a few jars lately that for some reason stalled. No problems but I only waited 3 weeks.
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