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The Medic
Stranger


Registered: 06/08/15
Posts: 29
Last seen: 8 years, 4 months
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WBR - GE, am I screwed?
#21842728 - 06/22/15 06:35 PM (8 years, 7 months ago) |
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The past few days I have been preparing WBR by the method of fractionalization, and last night inoculated the jars. However, reading around, it appears I may have made an unfortunate miscalculation.
I assumed that since in the PF-Tek you can cover the jars with foil, you could do the same with grains. However, upon doing some (late) research, it seems this is not the case. I currently have 0 gas exchange in my jars.
I have no polyfil or tyvek filter. Should I leave the jars be and hope they do okay, or should I pull off the foil (I'm using jars with 5 holes in the top lid, and put a layer of foil over that) and try to improvise something?
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: WBR - GE, am I screwed? [Re: The Medic]
#21842803 - 06/22/15 06:53 PM (8 years, 7 months ago) |
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There is probably some GE occuring, though not a lot.
The reason foil works with PF tek is due to the vermiculite contam barrier ontop of the cake, which isn't there for grains.
If your inoculation method is fast (LC/G2G/LI) you will probably be fine with low GE.
If it's slower, you may stall. Leave it and see what happens, or get an SAB and put some alcohol swabbed postal tyvek filter disks on.
If you do leave the foil, loosen it to provide some GE once you near 100% and cannot contaminate.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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The Medic
Stranger


Registered: 06/08/15
Posts: 29
Last seen: 8 years, 4 months
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Thanks for the advice. I used a syringe, so I may just have to see how fast my jars colonize and go from there.
Is it a feasible strategy to take the parts of the jar that have colonized, spawn them, and get rid of the rest of the rice that hasn't yet colonized? I'm just thinking that if my jars stall at something like 50% it'd be nice to have at least SOME mycelium come through for me.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: WBR - GE, am I screwed? [Re: The Medic]
#21842884 - 06/22/15 07:12 PM (8 years, 7 months ago) |
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I've done that with a few jars that had maybe 10% volume strip of bacterial contamination. Worked out fine. The more uncolonized matter you need to remove, the harder it will be to do a good job, and you definitely want it removed as it will contam extremely easily if it gets mixed with your bulk.
Personally I'd make a still air box, even if it's just a cardboad box with two holes and plastic wrap ontop so you can see. You can make some postal tyvek disc cutouts, sanitize with alcohol, remove the foil and jar ringe BUT LEAVE THE LID, place the tvek filter ontop of the lid, then replace the jar ring. This way only the hole in the lid is opened in still air conditions, which is almost 0 risk.
There's a good chance your stuff will be fine as is though.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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