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OfflinePhishies
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Registered: 06/15/15
Posts: 8
Last seen: 8 years, 6 months
Decade old spore print...
    #21842260 - 06/22/15 04:34 PM (8 years, 7 months ago)

Anyone think this print I made about 10 years ago is still viable. It's been stored in a dark, cool, and dry place. Still looks good but should I waste my time and supplies on it?



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InvisibleGrey
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Re: Decade old spore print... [Re: Phishies]
    #21842270 - 06/22/15 04:39 PM (8 years, 7 months ago)

I vote try it out.

:havesomescience:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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Invisiblemaddchef
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Re: Decade old spore print... [Re: Phishies]
    #21842271 - 06/22/15 04:39 PM (8 years, 7 months ago)

You may have to force hydrate the spores under a slight pressure.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflinePhishies
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Re: Decade old spore print... [Re: maddchef]
    #21842451 - 06/22/15 05:30 PM (8 years, 7 months ago)

Quote:

maddchef said:
You may have to force hydrate the spores under a slight pressure.




Not sure how to go about that. Any recommendations?


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Decade old spore print... [Re: Phishies]
    #21842456 - 06/22/15 05:32 PM (8 years, 7 months ago)

heck yea do some agar work with them


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Invisibleoontribe
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Re: Decade old spore print... [Re: mrbart4444]
    #21842472 - 06/22/15 05:35 PM (8 years, 7 months ago)

Load em in a strile water syringe and leave em for 24hrs.


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OfflinePhishies
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Re: Decade old spore print... [Re: mrbart4444]
    #21842477 - 06/22/15 05:35 PM (8 years, 7 months ago)

Quote:

mrbart4444 said:
heck yea do some agar work with them




Not set up to do that kind of work here.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Decade old spore print... [Re: Phishies]
    #21842482 - 06/22/15 05:37 PM (8 years, 7 months ago)

dang what you got going?


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Invisiblemaddchef
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Re: Decade old spore print... [Re: mrbart4444]
    #21842497 - 06/22/15 05:41 PM (8 years, 7 months ago)

Forced hydration was done by willsolvem I believe. He made a syringe with old spores that wouldn't germinate and filled the syringe, put a rubber band on in such a way it put slight pressure on the plunger and left it a few days.

I imagine the same could be done in a jar with only a single ship. Add spores, add water, inject sterile air to pressurize.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/22/15 06:03 PM)


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OfflinePhishies
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Registered: 06/15/15
Posts: 8
Last seen: 8 years, 6 months
Re: Decade old spore print... [Re: maddchef]
    #21842504 - 06/22/15 05:44 PM (8 years, 7 months ago)

Quote:

maddchef said:
Forced hydration was done by willsolvem I believe. He made a syringe with old spores that wouldn't germinate and called the syringe, put a rubber band on in such a way it put slight pressure on the plunger and left it a few days.

I imagine the same could be done in a jar with only a single ship. Add spores, add water, inject sterile air to pressurize.




Sounds interesting. Maybe I can pull one syringe after 24 hrs of hydration and then apply some air pressure to see if there is any difference.  thanks


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Decade old spore print... [Re: Phishies]
    #21842533 - 06/22/15 05:50 PM (8 years, 7 months ago)

maybe put some malt in the syringe you make for the spores? or some karo
Give them a chance if they really want it


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InvisibleCH HELL
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Re: Decade old spore print... [Re: mrbart4444]
    #21842559 - 06/22/15 05:55 PM (8 years, 7 months ago)

If the spores are just a cube and not something special to you I would NOT do it.  If it is a special print to you give it a try.  Anything over five years is not worth the trouble IMO.

CH


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InvisibleGrey
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Re: Decade old spore print... [Re: CH HELL]
    #21843123 - 06/22/15 07:59 PM (8 years, 7 months ago)

Stropharis had a write-up quoting the forced hydration technique. Just make the syringe, replace needle with syringe cap, and put weight on the plunger with the syringe resting vertically. Rubber band will probably work too, but just leave them to hydrate for a day or two before using... might work if there's any viability left.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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Invisiblemaddchef
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Registered: 09/04/09
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Re: Decade old spore print... [Re: Grey]
    #21843213 - 06/22/15 08:16 PM (8 years, 7 months ago)

Maybe it was stro then. It's been a long time since I ran across it.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisibleGrey
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Posts: 6,223
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Re: Decade old spore print... [Re: maddchef]
    #21843288 - 06/22/15 08:30 PM (8 years, 7 months ago)

It was someone else, he just quotes the write up in his write up.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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InvisibleStropharis
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Re: Decade old spore print... [Re: Grey]
    #21845969 - 06/23/15 01:04 PM (8 years, 7 months ago)

Stro's Spore Salvage with Forced Hydration
Stro's Spore Salvage with Malt Yeast Carbon Agar (MYCA)

The originality in the write up I did on forced hydration was the simplicity of the rubber band method. Both Auxin and RR discussed forced hydration prior to my writeup.

It's not impossible but it is very unlikely you will get those to germinate. Remember, the drop in spore viability is a ratio game, so don't be shy with them, keep us updated.


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Invisiblemaddchef
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Re: Decade old spore print... [Re: Stropharis]
    #21845982 - 06/23/15 01:08 PM (8 years, 7 months ago)

Good stuff stro. Glad to see you're around lurking.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflinePhishies
Stranger


Registered: 06/15/15
Posts: 8
Last seen: 8 years, 6 months
Re: Decade old spore print... [Re: Stropharis]
    #21846199 - 06/23/15 02:03 PM (8 years, 7 months ago)

Quote:

Stropharis said:
Stro's Spore Salvage with Forced Hydration
Stro's Spore Salvage with Malt Yeast Carbon Agar (MYCA)

The originality in the write up I did on forced hydration was the simplicity of the rubber band method. Both Auxin and RR discussed forced hydration prior to my writeup.

It's not impossible but it is very unlikely you will get those to germinate. Remember, the drop in spore viability is a ratio game, so don't be shy with them, keep us updated.




Much thanks, it's worth a shot.


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