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Offlinemushpunx
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Re: Need advise on Inoculation with Agar Wedge Vs LI Vs LC [Re: MysticPop]
    #21842253 - 06/22/15 04:31 PM (8 years, 7 months ago)

Quote:

MysticPop said:
So if i have 2 monotubs of 60 QT size.. How many Quart jars should i inoculate?




You will want 4 - 5 qts per tub. So if you are droppimg an agar wedge, you could get away with innoculating one master jar to G2G into 10 reciving jars.

If you are going all A2G you will have to inoculate 8-10 jars with wedge.

LC or LI same thing, inoculate 8-10 jars.


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OfflineMysticPop
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Registered: 05/05/15
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Re: Need advise on Inoculation with Agar Wedge Vs LI Vs LC [Re: mushpunx]
    #21842300 - 06/22/15 04:47 PM (8 years, 7 months ago)

So i plan to inoculate 4-6 jars with the plates i have.. Then depending on the quality of inoculation.. I could g2g from one that is the best? I also have around 12-14 plates that are currently inoculated and resting after my A2A transfer from yesterday..

Also why is G2G considered difficult than A2G?


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Offlinemushpunx
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Re: Need advise on Inoculation with Agar Wedge Vs LI Vs LC [Re: MysticPop]
    #21842546 - 06/22/15 05:53 PM (8 years, 7 months ago)

Quote:

MysticPop said:
So i plan to inoculate 4-6 jars with the plates i have.. Then depending on the quality of inoculation.. I could g2g from one that is the best? I also have around 12-14 plates that are currently inoculated and resting after my A2A transfer from yesterday..

Also why is G2G considered difficult than A2G?





You wont likely be able to pick out which one is "best" unless some go green or something. Contams hide so well in spawn you dont usually see contamination untill you fruit your tubs.

So it would be better to either inoculate one jar, and use it to G2G into the rest of the reciving jars or just drop a wedge from the plate into each of the jars going into one tub. One or the other.

Either way make sure your plate is clean, when in doubt make another transfer or two. I usually make 3 transfers before going to grain if its from spore.

A2G is easier because you are just quickly dropping a wedge into a jar and closing it back up. G2G is tricky because of the motions.. you might accidently slip your hand over the open jar when pouring, or if you didnt wipe the jar down good enough knock contams into the reciving jar etc..
Its not that hard just a little tricky. IME most contams get into spawn during G2G so you wanna make sure your technique is spot on.


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Invisiblemrbart4444
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Registered: 09/13/14
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Re: Need advise on Inoculation with Agar Wedge Vs LI Vs LC [Re: MysticPop]
    #21842565 - 06/22/15 05:58 PM (8 years, 7 months ago)

I myself like to innoc a lc wih my agar and then make syringes from the growth . I dont like g2g its way easier to just inject the material


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Edited by mrbart4444 (06/22/15 05:59 PM)


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