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InvisibleGrey
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
    #21807430 - 06/14/15 06:02 PM (9 years, 7 months ago)

:whathesaid:

Avoid anywhere that is obviously bacterial. Some have clean growth, but There a couple nasty ones I'd avoid. The first and last plate both have bacteria, but also have good looking growth. 12 o clock on the first plate and nine on the last. The second and fifth I wouldn't bother with.


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InvisibleGrey
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
    #21807437 - 06/14/15 06:04 PM (9 years, 7 months ago)

If it has bacteria, don't let it grow. Trash it. Your in the process of getting a clean culture. Once that's a success you can expand further.


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:takingnotes:  AMU Q&A  :takingnotes:


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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
    #21807443 - 06/14/15 06:07 PM (9 years, 7 months ago)

Where is bacteria in the first plate? I wish to know, how to identify bacteria.. I thought bacteria would be yellow. I will discard the 2nd and the 5th plate. What about between 4 and 9 in the third plate?

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InvisibleGrey
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21807509 - 06/14/15 06:24 PM (9 years, 7 months ago)

6 o clock on that first plate. The mycelium is very weak there. Even if it were clean, I wouldn't transfer from there when directly above it looks great.

The third plate has good growth, but also has weak or spotty growth. 6 and 8-8:30 on the plate is where the mycelium fans out looks the best to me.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


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InvisiblebodhisattaMDiscordReddit
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Grey]
    #21807528 - 06/14/15 06:29 PM (9 years, 7 months ago)

:popcorn:

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: bodhisatta]
    #21820811 - 06/17/15 08:51 PM (9 years, 6 months ago)

What do you think about these guys?


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InvisibleMad Season
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21821615 - 06/18/15 12:12 AM (9 years, 6 months ago)

First has those gross bacterial spots around the edges of the plate from 12 o'clock the 3. I wouldn't use this one since you have clean plates.

2nd has a big bacterial blotch at the 3-6 o'clock position

3rd looks great for grain inoculation and transfers from 1 o'clock to 7 o'clock.

4th looks good for grain and transfers from 10-5 o'clock position

5th is too disorganized for my liking

6th is clean and healthy and looks aggressive everywhere except the right hand side in my opinion.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleTheEaglesGift
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: Mad Season]
    #21821721 - 06/18/15 12:39 AM (9 years, 6 months ago)

You should change the title. It's a little misleading considering this isn't a guide.

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: TheEaglesGift]
    #21821980 - 06/18/15 01:50 AM (9 years, 6 months ago)

can u advise what is it? I am providing steps and what to expect in a step by step manner.. It also has links to videos etc. It is currently in progress. once completed, i think it will be a pretty comprehensive guide.

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Offlineshellzenone
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21821995 - 06/18/15 01:55 AM (9 years, 6 months ago)

Nice work mystic!:thumbup:

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InvisibleTheEaglesGift
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21822013 - 06/18/15 02:00 AM (9 years, 6 months ago)

I've never built an engine. I've also never written a guide on how to build an engine. If I were to write a guide to build an engine, it wouldn't be as I was building my first engine.

If I were to build an engine for the firs time, I wouldn't want the guide I was following to be written by someone who has never built and engine, or who was in the process of building one for the first time.

You have to see what I'm getting at.

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21822032 - 06/18/15 02:07 AM (9 years, 6 months ago)

Hi

I had changed the name of the guide to "Step By Step Cultivation Guide for Beginners BY a Beginner using Agar and Monotub!! " BY A BEGINNER..somehow it doesnot change the topic on the comments..

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InvisibleTheEaglesGift
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21822048 - 06/18/15 02:11 AM (9 years, 6 months ago)

Oh, well I didn't see that. Sorry for nitpicking you then. My bad. :lol:

It's a good thread, regardless. It'll be nice to see it finished up. :thumbup:

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: TheEaglesGift]
    #21822057 - 06/18/15 02:12 AM (9 years, 6 months ago)

nevermind...have fun

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OfflineMysticPop
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21840012 - 06/21/15 11:49 PM (9 years, 6 months ago)

Hey guys,

I did the transfers. I am left with the following plates. Can any of them be used for grain inoculation? PLease advise.


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Offlinespacechildo
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: MysticPop]
    #21840235 - 06/22/15 12:53 AM (9 years, 6 months ago)

its the plates you put the wedges you cut out onto that's interesting for us.
those plates there shouldn't be used. and you should have taken smaller wedges, as small as you can.

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InvisibleMad Season
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Re: [IN PROGRESS] Step By Step Guide for Beginners using Agar!! [Re: spacechildo]
    #21840860 - 06/22/15 07:50 AM (9 years, 6 months ago)

:whathesaid: you shouldn't transfer to grain jars now. They've been opened and transferred. Next time you transfer, if everything is clean, go ahead and inoculate jars.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3  [ show all ]

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