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OfflineMysticPop
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Need Advise if i Can use the leftover plates for grain Inoculation
    #21840040 - 06/21/15 11:56 PM (9 years, 6 months ago)

Hi All

I did the transfers. I am left with the following plates. Can any of them be used for grain inoculation? PLease advise.



Cheers Mystic

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Invisiblemaddchef
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840073 - 06/22/15 12:02 AM (9 years, 6 months ago)

What species is this? That is some thick mycelium.


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OfflineMysticPop
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: maddchef]
    #21840084 - 06/22/15 12:03 AM (9 years, 6 months ago)

gt

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Invisiblewildernessjunkie
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840094 - 06/22/15 12:06 AM (9 years, 6 months ago)

Yes. Absolutely.

GT is not a species. Its a variety of P. Cubensis.

You'll end up with the equivilant of a MS inoculation. Because its the same thing.

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OfflineMysticPop
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840120 - 06/22/15 12:13 AM (9 years, 6 months ago)

So which sectors do i use for grain inoculation? i understand we need rhizophoric strain only.. so what sector and which plate is the best?

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Invisiblewildernessjunkie
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840166 - 06/22/15 12:27 AM (9 years, 6 months ago)

IMO. And I stress that this is IMO. Rhizomorphic growth has more to do with available nutrition. The secondary factor is genetics in this case. A lot of people will disagree with me on this.

I prefer to do MS grows to find clones rather than isolate. Ill let mother nature do the work for me. The limited amount of P value saved is insignificant in my eyes.

If that plate was mine, I would use it to inoculate 3-4 grain jars and then make some dishes of the most promising results. From there I would make some slants.

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Invisiblewildernessjunkie
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840216 - 06/22/15 12:46 AM (9 years, 6 months ago)

Hmmm..reading my response again makes me think I was a little unclear.

I would cut the whole plate into wedges and use the whole thing to inoculate jars. Then I would look for promising clones.

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OfflineMysticPop
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840220 - 06/22/15 12:47 AM (9 years, 6 months ago)

What is MS? Also can you please advise plate by plate as too what i Could do? What sectors do i put in the grain Spawn jars? Can i Expect Pins from an of these plates? I have already done the Transfers of the most Rhizophoric strains to new agar dishes.

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Invisiblewildernessjunkie
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840313 - 06/22/15 01:18 AM (9 years, 6 months ago)

MS = Multispore

My point is to ignore the sectors. Cut each plate into 4 big wedges. Use them to inoculate 4 jars. Then select clones when they grow out. Though any of those plates is likely to produce pins.

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OfflineMysticPop
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840374 - 06/22/15 01:49 AM (9 years, 6 months ago)

what do you mean by select clones?

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OfflineMysticPop
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840387 - 06/22/15 01:53 AM (9 years, 6 months ago)

also i am assuming pinning is good rt?

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