Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Mono Tub Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Need Advise if i Can use the leftover plates for grain Inoculation
    #21840040 - 06/21/15 11:56 PM (8 years, 7 months ago)

Hi All

I did the transfers. I am left with the following plates. Can any of them be used for grain inoculation? PLease advise.



Cheers Mystic


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840073 - 06/22/15 12:02 AM (8 years, 7 months ago)

What species is this? That is some thick mycelium.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: maddchef]
    #21840084 - 06/22/15 12:03 AM (8 years, 7 months ago)

gt


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840094 - 06/22/15 12:06 AM (8 years, 7 months ago)

Yes. Absolutely.

GT is not a species. Its a variety of P. Cubensis.

You'll end up with the equivilant of a MS inoculation. Because its the same thing.


Extras: Filter Print Post Top
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840120 - 06/22/15 12:13 AM (8 years, 7 months ago)

So which sectors do i use for grain inoculation? i understand we need rhizophoric strain only.. so what sector and which plate is the best?


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840166 - 06/22/15 12:27 AM (8 years, 7 months ago)

IMO. And I stress that this is IMO. Rhizomorphic growth has more to do with available nutrition. The secondary factor is genetics in this case. A lot of people will disagree with me on this.

I prefer to do MS grows to find clones rather than isolate. Ill let mother nature do the work for me. The limited amount of P value saved is insignificant in my eyes.

If that plate was mine, I would use it to inoculate 3-4 grain jars and then make some dishes of the most promising results. From there I would make some slants.


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840216 - 06/22/15 12:46 AM (8 years, 7 months ago)

Hmmm..reading my response again makes me think I was a little unclear.

I would cut the whole plate into wedges and use the whole thing to inoculate jars. Then I would look for promising clones.


Extras: Filter Print Post Top
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840220 - 06/22/15 12:47 AM (8 years, 7 months ago)

What is MS? Also can you please advise plate by plate as too what i Could do? What sectors do i put in the grain Spawn jars? Can i Expect Pins from an of these plates? I have already done the Transfers of the most Rhizophoric strains to new agar dishes.


Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840313 - 06/22/15 01:18 AM (8 years, 7 months ago)

MS = Multispore

My point is to ignore the sectors. Cut each plate into 4 big wedges. Use them to inoculate 4 jars. Then select clones when they grow out. Though any of those plates is likely to produce pins.


Extras: Filter Print Post Top
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
    #21840374 - 06/22/15 01:49 AM (8 years, 7 months ago)

what do you mean by select clones?


Extras: Filter Print Post Top
OfflineMysticPop
AlienInWonderland
 User Gallery


Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
    #21840387 - 06/22/15 01:53 AM (8 years, 7 months ago)

also i am assuming pinning is good rt?


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Mono Tub Substrate


Similar ThreadsPosterViewsRepliesLast post
* Massive grain inoculation Scourge 1,221 8 05/21/01 05:27 PM
by snarf
* Agar inoculation? tatter1090 1,892 7 01/23/05 03:34 PM
by MushroomFriend
* Grain to Grain kindkesey 2,335 13 03/03/04 07:50 PM
by Anonymous
* Inoculating with mushed up shroom tissue..?
( 1 2 all )
Openminded 7,260 34 06/26/17 09:37 AM
by bodhisatta
* Streaking technique and Plastic inoculation loops mesq 4,509 7 04/12/03 10:27 AM
by no-tone
* Agar...Rye...Straw...Grain... Quik_N_Kool 1,576 7 10/02/03 04:33 AM
by Baby_Hitler
* Awesome rye grain h2o2 tek dimitri211 11,704 1 08/17/01 08:28 PM
by jonnyshaggs420
* basic grain theory
( 1 2 all )
TerrapinSunrise 4,171 36 03/04/05 03:03 PM
by Hotnuts

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
570 topic views. 31 members, 299 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.009 seconds on 14 queries.