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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Need Advise if i Can use the leftover plates for grain Inoculation
#21840040 - 06/21/15 11:56 PM (8 years, 7 months ago) |
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Hi All
I did the transfers. I am left with the following plates. Can any of them be used for grain inoculation? PLease advise.

Cheers Mystic
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
#21840073 - 06/22/15 12:02 AM (8 years, 7 months ago) |
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What species is this? That is some thick mycelium.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: maddchef]
#21840084 - 06/22/15 12:03 AM (8 years, 7 months ago) |
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gt
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
#21840094 - 06/22/15 12:06 AM (8 years, 7 months ago) |
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Yes. Absolutely.
GT is not a species. Its a variety of P. Cubensis.
You'll end up with the equivilant of a MS inoculation. Because its the same thing.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
#21840120 - 06/22/15 12:13 AM (8 years, 7 months ago) |
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So which sectors do i use for grain inoculation? i understand we need rhizophoric strain only.. so what sector and which plate is the best?
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
#21840166 - 06/22/15 12:27 AM (8 years, 7 months ago) |
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IMO. And I stress that this is IMO. Rhizomorphic growth has more to do with available nutrition. The secondary factor is genetics in this case. A lot of people will disagree with me on this.
I prefer to do MS grows to find clones rather than isolate. Ill let mother nature do the work for me. The limited amount of P value saved is insignificant in my eyes.
If that plate was mine, I would use it to inoculate 3-4 grain jars and then make some dishes of the most promising results. From there I would make some slants.
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wildernessjunkie
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Registered: 06/13/10
Posts: 8,118
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
#21840216 - 06/22/15 12:46 AM (8 years, 7 months ago) |
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Hmmm..reading my response again makes me think I was a little unclear.
I would cut the whole plate into wedges and use the whole thing to inoculate jars. Then I would look for promising clones.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
#21840220 - 06/22/15 12:47 AM (8 years, 7 months ago) |
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What is MS? Also can you please advise plate by plate as too what i Could do? What sectors do i put in the grain Spawn jars? Can i Expect Pins from an of these plates? I have already done the Transfers of the most Rhizophoric strains to new agar dishes.
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
#21840313 - 06/22/15 01:18 AM (8 years, 7 months ago) |
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MS = Multispore
My point is to ignore the sectors. Cut each plate into 4 big wedges. Use them to inoculate 4 jars. Then select clones when they grow out. Though any of those plates is likely to produce pins.
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: wildernessjunkie]
#21840374 - 06/22/15 01:49 AM (8 years, 7 months ago) |
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what do you mean by select clones?
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MysticPop
AlienInWonderland



Registered: 05/05/15
Posts: 132
Last seen: 2 months, 30 days
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Re: Need Advise if i Can use the leftover plates for grain Inoculation [Re: MysticPop]
#21840387 - 06/22/15 01:53 AM (8 years, 7 months ago) |
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also i am assuming pinning is good rt?
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