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Offlinebobmarley4prez
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can liquid culture be done with sucrose?
    #2183859 - 12/16/03 10:30 PM (12 years, 11 months ago)

can you use table sugar and water for a liquid growth medium, and what are the proportions?
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Anything above this line is a lie. i do not cultivate psychadelic mushrooms nore do I intend to.

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Invisiblemicro
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Re: can liquid culture be done with sucrose? [Re: bobmarley4prez]
    #2184040 - 12/16/03 11:31 PM (12 years, 11 months ago)

Yeah -- it should work -- it might not be as good, but it should definately work. I would use like 4% by weight.

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Offlinellamaboy
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Re: can liquid culture be done with sucrose? [Re: bobmarley4prez]
    #2184252 - 12/17/03 01:24 AM (12 years, 11 months ago)

why just not use something like honey, which is also readily available? table sugar can't be good to use...it's been bleached and what not...unless you're using that brown looking sucrose, but that still has the mollassas(spelling) in it.


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OfflineRandolph_Carter
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Re: can liquid culture be done with sucrose? [Re: bobmarley4prez]
    #2184257 - 12/17/03 01:26 AM (12 years, 11 months ago)

Any simple sugar in water will grow mycellium....at concentratons >10%, it becomes lethal. Personally, i start mine with bout 6-8 precent from honey.


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Offlinellamaboy
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Re: can liquid culture be done with sucrose? [Re: Randolph_Carter]
    #2184306 - 12/17/03 01:48 AM (12 years, 11 months ago)

well, but table sugar isnt' your ordinary sugar...its' been fucked with...so would that still be okay to use?


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OfflineRandolph_Carter
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Re: can liquid culture be done with sucrose? [Re: llamaboy]
    #2184310 - 12/17/03 01:50 AM (12 years, 11 months ago)

probably wouldn't hurt to try...i don't see any problems with it...


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Nuke baby seals for Jesus!

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OfflineBrokenBeast
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Re: can liquid culture be done with sucrose? [Re: Randolph_Carter]
    #2187310 - 12/18/03 09:15 AM (12 years, 11 months ago)

Glad someone asked this, I've always wondered...
(Not bothered trying because dextrose is dirt cheap at my supermarket)


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OfflineBaby_Hitler
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Re: can liquid culture be done with sucrose? [Re: bobmarley4prez]
    #2187321 - 12/18/03 09:35 AM (12 years, 11 months ago)

I know Fructose will work. You might find some of that in the health food / diet section.

I usually use potato flakes for a source of nutrients and fructose as a source of energy for the fungus.


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OfflineMages
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Re: can liquid culture be done with sucrose? [Re: Baby_Hitler]
    #2193627 - 12/20/03 11:05 PM (12 years, 11 months ago)

You can also find fructose in beermaking/winemaking shops.


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OfflineBaby_Hitler
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Re: can liquid culture be done with sucrose? [Re: Mages]
    #2193633 - 12/20/03 11:09 PM (12 years, 11 months ago)

If you have that option, then I would look for dextrose first.


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OfflineMages
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Re: can liquid culture be done with sucrose? [Re: Baby_Hitler]
    #2194300 - 12/21/03 08:20 PM (12 years, 11 months ago)

excellent point.
- Mages


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OfflineGranola
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Re: can liquid culture be done with sucrose? [Re: bobmarley4prez]
    #2194747 - 12/22/03 12:53 AM (12 years, 11 months ago)

one project I had done, I boiled corn for spawn and used the water that was left over for a liquid inocculum, it worked very well and kept for a couple of weeks in the fridge. I diluted it to 50% and PC'd it with the spawn. it was difficult to see because it caramelized in the PC. Sucrose will work, but try to keep it at 5%, think about it, often times spore germinate in water alone.


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Offline284_27
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Re: can liquid culture be done with sucrose? [Re: Randolph_Carter]
    #2203547 - 12/27/03 08:06 AM (12 years, 11 months ago)

Can someone tell me where the information about the concentration of sugars comes from ? I would like to see someone do a study to find out which concentration of Karo or some other liquid culture has the fastest colonization speed , most prone to contams etc . From my what a friend told me he uses about 10ml of karo to 240 ml for the fastest speeds .


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Offlineragadinks
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Re: can liquid culture be done with sucrose? [Re: 284_27]
    #2203604 - 12/27/03 09:58 AM (12 years, 11 months ago)

Here are two short articles about liquid spawn:
http://www.mushworld.com/tech/view.asp?cata_id=1140&vid=4825
http://www.mushworld.com/tech/view.asp?cata_id=1290&vid=542

I no that I have read somewhere that a solution of 30 g dextrose and 2 g yeast in 1 liter of water produced maximum yields of mycelium for a certain species. But I cannot find that article anymore. Anyway, I have tried it out and compared to pure honey it produces really more mycelium in a shorter amount of time.

Concerning contamination in liquid cultures: I would recommend to work in a *very* clean environment anyway! Regardless what liquid substrate you use, they are all very prone to contams.


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