Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJsneeb
Drifter
 User Gallery

Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Myc piss or contam?
    #21832304 - 06/20/15 12:54 PM (8 years, 7 months ago)

So I prepped my Monotub last Wednesday.
The substrate is three parts Horse manure and coir to two parts spawn.
Overall about 12 quarts of substrate.

I packaged it all up. Taped the bottom holes, tight poly in the top, and sealed it in a fresh, disinfected black trash bag for 3 days. So its been 72 hours and I opened it up today.

Looks beautifully colonized, Except in one corner a significant amount of liquid has accumulated. It is very clear but yellow.

What is it? I suspect it came from condensation on the walls of the monotub. It looks like my substrate was a bit over on moisture content.

Next question. What should I do about casing layer? Can I fruit it as is and case on the second flush? Should I case now? Should I have cased earlier? This is my first attempt and not getting contams or if I do being able to sport them is top priority. Flush size and convenience is second to this.

Last question. You can see little bits of substrate. Nickle or dime size that are not colonized. Will these finish up and colonize within the next few days? I plan to keep it exposed to light but give it a week before I introduce FAE for fruiting.

Pictures bellow.







--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


Extras: Filter Print Post Top
OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 5 years, 10 months
Trusted Cultivator
Re: Myc piss or contam? [Re: Jsneeb]
    #21832320 - 06/20/15 12:58 PM (8 years, 7 months ago)

Looks ready to case, i wouldnt say your late at all. Those uncolonized spots will colonize under the casing. The yellow water is normal and its from the mushrooms, its how they break down and digest the substrate.


Extras: Filter Print Post Top
OfflineJsneeb
Drifter
 User Gallery

Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Myc piss or contam? [Re: Gr0wer]
    #21832572 - 06/20/15 02:22 PM (8 years, 7 months ago)

Sweet. I soaked it up with a loose paper towel. I'll go ahead and case tomorrow. Any recommended Teks?


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


Extras: Filter Print Post Top
OfflineTravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
Re: Myc piss or contam? [Re: Jsneeb]
    #21832589 - 06/20/15 02:28 PM (8 years, 7 months ago)

Are you sure? That looks slimy to me...


--------------------





Indoor Greenhouse Build 
          Mid Size Mushroom Farm


Extras: Filter Print Post Top
OfflineFunnyLight
Nom NOm NOM
Male User Gallery


Registered: 09/12/11
Posts: 1,124
Loc: fuckin Mars man
Last seen: 1 year, 8 months
Re: Myc piss or contam? [Re: TravelAgency]
    #21832879 - 06/20/15 03:57 PM (8 years, 7 months ago)

I would wait on the casing. It's normal to see some wetting like that, but that's a lot. If you have a contam that the fungus is fighting and you case. You trap that contam in your substrate. If the fungi fails to combat it, then you won't be able to spot it as soon as you would if you didn't case.


Also, don't worry about casing cubes. It's a complicated debate/preference. But if your just getting up and running, I'd hold off casing until you've got a handle on the process.

Good Luck!


--------------------
The most powerful drive through the ascent of man, is pleasure in his own skill.
J. Bronowski

Home of delicious "Psychedelic Nyotaimori".
Thanks Lemmingp for that.

:mushroom2::grin::mushroom2:


Extras: Filter Print Post Top
OfflineJsneeb
Drifter
 User Gallery

Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Myc piss or contam? [Re: FunnyLight]
    #21833129 - 06/20/15 05:18 PM (8 years, 7 months ago)

FunnyLight, So would you recommend not casing at all then? or just waiting a few extra days to make sure its all clear?


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Myc piss or contam? [Re: Jsneeb]
    #21833783 - 06/20/15 08:19 PM (8 years, 7 months ago)

If you feel you're inexperienced, don't case. It's way better and a lot more experience imo to not case for the first few times. That way you get to see the mycelium more, how it looks and behaves. Also get to see what to look for and what not to look for. Gets a good handle on misting too. Way better for noobies imo.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Myc piss or contam? [Re: Mad Season]
    #21834410 - 06/20/15 10:33 PM (8 years, 7 months ago)

They're metabolites, and a lot of them.

I'd case it. I've started casing all of my subs. I love it.

What temp are you colonizing your substrate?


Extras: Filter Print Post Top
OfflineJsneeb
Drifter
 User Gallery

Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Myc piss or contam? [Re: TheEaglesGift]
    #21838946 - 06/21/15 08:13 PM (8 years, 7 months ago)

Hmm I'm really torn here guys on whether to case or not now.

What actually are the benefits? Just created a better micro climate for a better pinset?

I was colonizing at about 82-85 degrees for the first 72 hours after spawning. On one occasion my heater was set wrong and it got up to about 92 for perhaps 3 hours. Looks like the Sub made it though. I suspect interior temps where even higher as its been colonizing very fast.
Now I'm down to room temp, which with the sun heating that room a but during the day fluctuates between 70 and 76.

My agar didn't grow well at all under 85 degrees. Once I got it warm everything took off.


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


Extras: Filter Print Post Top
Invisibledjmako7
Antitransubstantiationalist
 User Gallery


Registered: 06/13/06
Posts: 2,362
Loc: A shady spot under a tree
Re: Myc piss or contam? [Re: Jsneeb]
    #21838982 - 06/21/15 08:15 PM (8 years, 7 months ago)

Cubes dont need to be cased.


--------------------




Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Myc piss or contam? [Re: djmako7]
    #21839109 - 06/21/15 08:36 PM (8 years, 7 months ago)

They don't need to be, but proper casings can only be beneficial.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Myc piss or contam? [Re: Jsneeb]
    #21839500 - 06/21/15 09:50 PM (8 years, 7 months ago)

Quote:

Jsneeb said:
Hmm I'm really torn here guys on whether to case or not now.

What actually are the benefits? Just created a better micro climate for a better pinset?

I was colonizing at about 82-85 degrees for the first 72 hours after spawning. On one occasion my heater was set wrong and it got up to about 92 for perhaps 3 hours. Looks like the Sub made it though. I suspect interior temps where even higher as its been colonizing very fast.
Now I'm down to room temp, which with the sun heating that room a but during the day fluctuates between 70 and 76.

My agar didn't grow well at all under 85 degrees. Once I got it warm everything took off.



Those higher temps can certainly cause excessive metabolites. Metabolites are often a response to bacterial infection, and higher temps increase bacterial infections. Do the math. :thumbup:

Room temp, like you're using currently, is much better.

Quote:

Cubes dont need to be cased.



Cubensis do better with a casing layer in my experience. Cubes will certainly grow without it, though.


Edited by TheEaglesGift (06/21/15 09:56 PM)


Extras: Filter Print Post Top
Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: Myc piss or contam? [Re: Jsneeb]
    #21841063 - 06/22/15 09:40 AM (8 years, 7 months ago)

Quote:

Jsneeb said:

I packaged it all up. Taped the bottom holes, tight poly in the top, and sealed it in a fresh, disinfected black trash bag for 3 days. So its been 72 hours and I opened it up today.







When I was doing mono tubs I started off with the trash bag colonization method.  I don't recommend that anymore.  For me it trapped too much heat and would increase the temps to dangerous levels.  This was probably due to my room already being too warm.  I replaced the black trashbag with a clean towel that i draped over the tub.  BTW having some light hit your  tub while colonizing is not terrible.  Light is only a secondary pinning trigger while fruiting. 

Also i have never seen pollyfill used during colonizing.  I am assuming this was for gas exchange?
The small space between the lid and the tub itself is enough for GE.  Tape up all holes next time around and just latch the lid to the tub for GE.


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Myc piss or contam? [Re: shroomyaxn]
    #21841084 - 06/22/15 09:48 AM (8 years, 7 months ago)

I really hate the phrase mycelium piss.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: Myc piss or contam? [Re: FunnyLight]
    #21841090 - 06/22/15 09:50 AM (8 years, 7 months ago)

Quote:

FunnyLight said:
I would wait on the casing. It's normal to see some wetting like that, but that's a lot. If you have a contam that the fungus is fighting and you case. You trap that contam in your substrate. If the fungi fails to combat it, then you won't be able to spot it as soon as you would if you didn't case.

Also, don't worry about casing cubes. It's a complicated debate/preference. But if your just getting up and running, I'd hold off casing until you've got a handle on the process.





I completely agree with this.  The yellowing is due to the substrate fighting off contams.  I would wait until 7 days of colonization have passed then introduce to fruiting. Give lots of FAE at first, this will evaporate all that water vapor off the surface, creating a great pinning environment. 

Side note: I have recently learned that yellow metabolites appearing after full colonization (during fruiting conditions) can be heat stress related.  When it appears during colonization it usually relates to the subtrate fighting a contam.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Myc piss or contam? [Re: shroomyaxn]
    #21841094 - 06/22/15 09:52 AM (8 years, 7 months ago)

Light is beneficial, but I can argue it isn't a pinning trigger, since you can successfully grow in the dark. You don't have to worry about it too much. I mean do what you want, but that sounds like a waste of a towel, and a little unsanitary right next to the lid. If you see pinning before full colonization, it isn't light related. It's because of contaminations/too wet substrate. If you see side pinning, it's not because of light. It's because of the high surface humidity in that microclimate.

If you make a good substrate that's not too wet or dry, with lots of surface humidity, and a lower rh/high fae for evaporation, you'll have a great surface pinset regardless of light or liners.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/22/15 09:55 AM)


Extras: Filter Print Post Top
OfflineTrickyricky217
ThatOneGuy
Male


Registered: 04/26/15
Posts: 144
Loc: Midwest Flag
Last seen: 8 years, 1 month
Re: Myc piss or contam? [Re: Mad Season]
    #21841119 - 06/22/15 10:01 AM (8 years, 7 months ago)

I read somewhere that RR said metabolites usually appear for one or more of three reasons. Fighting an infection, over heating, and over colonized substrate


Extras: Filter Print Post Top
Offlineshroomyaxn
Male User Gallery


Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
Re: Myc piss or contam? [Re: Mad Season]
    #21841178 - 06/22/15 10:24 AM (8 years, 7 months ago)

Quote:

Mad Season said:
Light is beneficial, but I can argue it isn't a pinning trigger, since you can successfully grow in the dark. You don't have to worry about it too much. I mean do what you want, but that sounds like a waste of a towel, and a little unsanitary right next to the lid.




Agreed.  If I were to do Mono tubs again today, I would skip the towel.  An unopened trash bag could be laid over the tubs if your colonization area is dusty, like most places i tend to live in.  :frown:


Extras: Filter Print Post Top
Invisibleoontribe
Male User Gallery
Registered: 01/14/15
Posts: 3,570
Re: Myc piss or contam? [Re: shroomyaxn]
    #21841522 - 06/22/15 12:26 PM (8 years, 7 months ago)

Light gives you bigger fruits.


Extras: Filter Print Post Top
OfflineprofessorFATTYCAP
Training 4 the mycothalon
 User Gallery


Registered: 04/08/14
Posts: 750
Last seen: 2 months, 16 days
Re: Myc piss or contam? [Re: oontribe]
    #21841543 - 06/22/15 12:38 PM (8 years, 7 months ago)

eagle is rite case it. learn the process now .ive only ever cased and i love it.on a side note try to keep ur liner slightly above the surface this allows the condensation to run down behind it instead of on or down into ur sub which could invite nastys


Extras: Filter Print Post Top
OfflineJsneeb
Drifter
 User Gallery

Registered: 04/05/15
Posts: 109
Last seen: 6 years, 10 months
Re: Myc piss or contam? [Re: professorFATTYCAP]
    #21842646 - 06/22/15 06:16 PM (8 years, 7 months ago)

Thanks everyone for the info. Lots good knowledge here from both sides.

I'll also point out one more detail. The corner which has the most metabolites has a small spot, about the size of a quarter which is not colonized. Everything else is a solid mat of mycelium at this point.

I drew around it with sharpie yesterday and so far today it doesn't look like it has made any progress into the uncolonized area.

This section isnt too deep. Would it be worthwhile to sterilize a spoon and go in and remove that corner? I don't see anything and its encased top and bottom with Mycelium so I cant smell it but I suspect it could be bacteria just locally in that region.


--------------------
Read my trip report :smile:

Titled, Mushrooms, Thirsty Water Bottle, And Boiling Peanut-butter?

http://www.reddit.com/r/shrooms/comments/37z9rp/mushrooms_thirsty_water_bottle_and_boiling/


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Myc piss or contam? [Re: Jsneeb]
    #21842654 - 06/22/15 06:18 PM (8 years, 7 months ago)

I'm sure I'm not the only one that's picked out an uncolonized spot that was being stubborn. I'd say you can.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Myc piss or contam? [Re: Mad Season]
    #21842705 - 06/22/15 06:29 PM (8 years, 7 months ago)

Quote:

Mad Season said:
I'm sure I'm not the only one that's picked out an uncolonized spot that was being stubborn. I'd say you can.




Go for it.

Colonize your shit in lower temps and this won't happen often.

Quote:

Trickyricky217 said:
I read somewhere that RR said metabolites usually appear for one or more of three reasons. Fighting an infection, over heating, and over colonized substrate




Agreed. It's also worth noting that over heating INCREASED incidence of bacterial infection. The two aren't mutually exclusive.


Extras: Filter Print Post Top
OfflineprofessorFATTYCAP
Training 4 the mycothalon
 User Gallery


Registered: 04/08/14
Posts: 750
Last seen: 2 months, 16 days
Re: Myc piss or contam? [Re: Mad Season]
    #21842814 - 06/22/15 06:56 PM (8 years, 7 months ago)

Quote:

Mad Season said:
I'm sure I'm not the only one that's picked out an uncolonized spot that was being stubborn. I'd say you can.




ive pluckd out a spot here and there never an issue


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* myc piss criminulmindid 3,422 18 03/11/16 04:59 PM
by lukehighwalker710
* What is the least contam prone substrate? *DELETED* MeetMeInEleusis 1,274 4 03/24/02 11:59 AM
by HisStudent
* cake..contam removed.myc piss?.no pins..ne ideas OuTdOoR 1,380 4 08/28/02 06:03 PM
by OuTdOoR
* What is the MOST contam resistant substrate? Baby_Hitler 2,136 6 07/17/02 01:12 PM
by mile69
* Simple question about colonizing the substrate TheEggman 1,606 3 02/01/03 08:04 AM
by SubGen1us
* mycelium piss Zen Peddler 2,374 9 05/25/19 09:36 PM
by PnwCyAzurescens
* question on substrate mikearch 1,340 7 09/02/02 08:46 AM
by DreaMaTrix
* casing contam 1stimer 1,123 11 10/02/02 07:29 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,472 topic views. 35 members, 222 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.011 seconds on 14 queries.