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digdog
Stranger Than Average



Registered: 04/30/15
Posts: 44
Last seen: 8 years, 4 months
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Day 5 Agar Petri Dish Reishi Spore Growth
#21831638 - 06/20/15 08:22 AM (8 years, 7 months ago) |
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Hello all,
I am on day 5 with petri dishes that I inoculated with reishi spores.
I will save you having to read all the gory details but my initial pour of the agar is best described as "bumbling". I logged my folly in my journal.
However once I adjusted (got thin gloves) the inoculation went quick and without issue.
Here is one of my dishes on Day 5:

So what do you all think? Is this progressing normally? Am I in good shape so far?
TIA for your thoughts and expertise.
DigDog
-------------------- Once I stop learning, life will cease to have meaning.
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digdog
Stranger Than Average



Registered: 04/30/15
Posts: 44
Last seen: 8 years, 4 months
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: digdog]
#21831650 - 06/20/15 08:26 AM (8 years, 7 months ago) |
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Oh just an addition:
I am storing the dishes at about 78 degrees f and giving them ambient light in 12 on 12 off cycle.
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: digdog]
#21831653 - 06/20/15 08:29 AM (8 years, 7 months ago) |
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Looks clean, not sure what Reishi mycelium is supposed to look like though.
Also, people generally use saran wrap instead of taping dishes. Seems a little easier.
Try the search function sometime  I found you this...
http://www.shroomery.org/9398/Ganoderma-lucidum Spawn Run: Incubation Temperature: 70-80* F (21-27* C) Relative Humidity: 95-100% Duration: 10-20 days CO2: Tolerated up to 50,000 ppm or 5% Fresh Air Exchanges: 0-1 Light Requirements: n/a
Primordia ("Antler") Formation: Initiation Temperature: 65-75* F (18-24* C) Relative Humidity: 95-100% Duration: 14-28 days CO2: 20,000-40,000 ppm Fresh Air Exchanges: 0-1 Light Requirements: 4-8 hours at 200-500 lux
Primordia ("Young Conk") Formation: Temperature: 70-80* F (21-27* C) Relative Humidity: 95-100% Duration: 14-28 days CO2: 2000-5000 ppm Fresh Air Exchanges: As required for maintaining desired CO2. Light Requirements: 12 hours on/off 750-1500 lux.
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digdog
Stranger Than Average



Registered: 04/30/15
Posts: 44
Last seen: 8 years, 4 months
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: GreenRabbit]
#21831703 - 06/20/15 08:47 AM (8 years, 7 months ago) |
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GR,
Thanks. Yes, I used Glad Cling Wrap per what a lot of the threads I found advised. 
I did search quite a bit before getting started. The gloves was really the only mistake I feel like I made (although I am sure I will make many more).
Thanks for the feed back. Glad they look clean. Feel like I dodged a big old bullet.
Thanks for the link to the growing specs as well.
DigDog
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