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Offlinedigdog
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Registered: 04/30/15
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Last seen: 8 years, 4 months
Day 5 Agar Petri Dish Reishi Spore Growth
    #21831638 - 06/20/15 08:22 AM (8 years, 7 months ago)

Hello all,

I am on day 5 with petri dishes that I inoculated with reishi spores.

I will save you having to read all the gory details but my initial pour of the agar is best described as "bumbling". I logged my folly in my journal.

However once I adjusted (got thin gloves) the inoculation went quick and without issue.

Here is one of my dishes on Day 5:



So what do you all think? Is this progressing normally? Am I in good shape so far?

TIA for your thoughts and expertise.

DigDog


--------------------
Once I stop learning, life will cease to have meaning.


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Offlinedigdog
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: digdog]
    #21831650 - 06/20/15 08:26 AM (8 years, 7 months ago)

Oh just an addition:

I am storing the dishes at about 78 degrees f and giving them ambient light in 12 on 12 off cycle.


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OfflineGreenRabbit
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: digdog]
    #21831653 - 06/20/15 08:29 AM (8 years, 7 months ago)

Looks clean, not sure what Reishi mycelium is supposed to look like though.

Also, people generally use saran wrap instead of taping dishes. Seems a little easier.

Try the search function sometime :stoned:
I found you this...

http://www.shroomery.org/9398/Ganoderma-lucidum
Spawn Run:
Incubation Temperature: 70-80* F (21-27* C)
Relative Humidity: 95-100%
Duration: 10-20 days
CO2: Tolerated up to 50,000 ppm or 5%
Fresh Air Exchanges: 0-1
Light Requirements: n/a

Primordia ("Antler") Formation:
Initiation Temperature: 65-75* F (18-24* C)
Relative Humidity: 95-100%
Duration: 14-28 days
CO2: 20,000-40,000 ppm
Fresh Air Exchanges: 0-1
Light Requirements: 4-8 hours at 200-500 lux

Primordia ("Young Conk") Formation:
Temperature: 70-80* F (21-27* C)
Relative Humidity: 95-100%
Duration: 14-28 days
CO2: 2000-5000 ppm
Fresh Air Exchanges: As required for maintaining desired CO2.
Light Requirements: 12 hours on/off 750-1500 lux.


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Offlinedigdog
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Re: Day 5 Agar Petri Dish Reishi Spore Growth [Re: GreenRabbit]
    #21831703 - 06/20/15 08:47 AM (8 years, 7 months ago)

GR,

Thanks. Yes, I used Glad Cling Wrap per what a lot of the threads I found advised. :smile:

I did search quite a bit before getting started. The gloves was really the only mistake I feel like I made (although I am sure I will make many more).

Thanks for the feed back. Glad they look clean. Feel like I dodged a big old bullet.

Thanks for the link to the growing specs as well.

DigDog


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