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Offlineblazedup
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Thoughts?
    #21829495 - 06/19/15 07:03 PM (8 years, 7 months ago)

So I check my cakes every few days to see if any changes in color and to make sure things look healthy. This guy didn't look too great. Looks like bruising from possibly picking it up but that could just be wishful thinking.



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Offlinesaintburger
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Re: Thoughts? [Re: blazedup]
    #21829532 - 06/19/15 07:12 PM (8 years, 7 months ago)

I'm no expert but I would probably toss that


--------------------
Set goals for yourself, or wander around aimlessly.

"One day Alice came to a fork in the road
and saw a Cheshsire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'"


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OfflineGreenRabbit
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Re: Thoughts? [Re: saintburger]
    #21829654 - 06/19/15 07:37 PM (8 years, 7 months ago)

Hold on to it for a couple days to see. It might be ok.. That jar looks a little wet though.


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Offlineblazedup
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Re: Thoughts? [Re: GreenRabbit]
    #21829672 - 06/19/15 07:39 PM (8 years, 7 months ago)

Quote:

GreenRabbit said:
Hold on to it for a couple days to see. It might be ok.. That jar looks a little wet though.




I separated it from the rest and placed it alone. I thought the same because of the droplets of water. I think my room I have them in gets way too hot and humid causing the condensation.


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OfflinePsilicon
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Re: Thoughts? [Re: blazedup]
    #21829949 - 06/19/15 08:37 PM (8 years, 7 months ago)

Those metabolites spell disaster.  Toss it before it sporulates.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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Offlineblazedup
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Re: Thoughts? [Re: Psilicon]
    #21830125 - 06/19/15 09:18 PM (8 years, 7 months ago)

Quote:

van der griegen said:
Those metabolites spell disaster.  Toss it before it sporulates.




So it's def a contam then


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OfflinePsilicon
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Re: Thoughts? [Re: blazedup]
    #21830135 - 06/19/15 09:20 PM (8 years, 7 months ago)

Quote:

blazedup said:
Quote:

van der griegen said:
Those metabolites spell disaster.  Toss it before it sporulates.




So it's def a contam then




It might be several contaminants, but there is definitely at least one.  Sorry, but I'm glad you have several healthy backups.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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Offlineblazedup
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Re: Thoughts? [Re: Psilicon]
    #21830146 - 06/19/15 09:23 PM (8 years, 7 months ago)

Good to know..I worry that the other ones may have the same fate. Because of how white the growth looks in some of them.


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Offlineblazedup
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Re: Thoughts? [Re: blazedup]
    #21830150 - 06/19/15 09:24 PM (8 years, 7 months ago)

For example...




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OfflinePsilicon
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Re: Thoughts? [Re: blazedup]
    #21830188 - 06/19/15 09:31 PM (8 years, 7 months ago)

That doesn't look like cubensis to me, but it definitely looks like some kind of mushroom.  I'd say not contaminated there.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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Offlineblazedup
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Re: Thoughts? [Re: Psilicon]
    #21830205 - 06/19/15 09:35 PM (8 years, 7 months ago)

Quote:

van der griegen said:
That doesn't look like cubensis to me, but it definitely looks like some kind of mushroom.  I'd say not contaminated there.




These cakes are either pan cyan or pan cam. I made a boo boo when making the syringe thing none of the spores got in the shot glass because I couldn't see them. (Noob move)

I was wondering if pans look different in the colonization period


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OfflinePsilicon
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Re: Thoughts? [Re: blazedup]
    #21830258 - 06/19/15 09:45 PM (8 years, 7 months ago)

I sure think so!


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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Offlineblazedup
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Re: Thoughts? [Re: Psilicon]
    #21846794 - 06/23/15 05:11 PM (8 years, 7 months ago)

I thought I would post pictures of how the jar looked when I went to dump it outside.



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Offlinespacechildo
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Re: Thoughts? [Re: blazedup]
    #21846807 - 06/23/15 05:16 PM (8 years, 7 months ago)

how did you make these "cakes"? the top verm layer looks soaking wet and it doesnt look like any tek I've ever seen.


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Offlineblazedup
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Re: Thoughts? [Re: spacechildo]
    #21846859 - 06/23/15 05:28 PM (8 years, 7 months ago)

Quote:

spacechildo said:
how did you make these "cakes"? the top verm layer looks soaking wet and it doesnt look like any tek I've ever seen.




I used the standard brf tek... Brf mixed with verm and a small amount of gypsum. Then top layer of dry verm. I believe the high temp and humidity caused moisture which started to build up. Then once the myc started to grow it began to go up to the dry verm layer.


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Offlinespacechildo
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Re: Thoughts? [Re: blazedup]
    #21846867 - 06/23/15 05:30 PM (8 years, 7 months ago)

the standard brf tek calls for short 1/2 pints not large plastic bowls, nor does it get the dry verm wet..


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Offlineblazedup
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Re: Thoughts? [Re: spacechildo]
    #21846893 - 06/23/15 05:37 PM (8 years, 7 months ago)

Quote:

spacechildo said:
the standard brf tek calls for short 1/2 pints not large plastic bowls, nor does it get the dry verm wet..




These are half pint plastic containers which I have used before. Also you can use practically any small container that is half pint for brf tek. I have even had success with tall pint jars. I didn't wet the dry verm layer. Condensation caused the verm to get wet.


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Offlinespacechildo
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Re: Thoughts? [Re: blazedup]
    #21846912 - 06/23/15 05:44 PM (8 years, 7 months ago)

if you've succeeded with those containers before just do what you did back then to avoid this.

I suspect there are some other tweaks you've done as well, inoc holes f.ex.


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Offlineblazedup
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Re: Thoughts? [Re: spacechildo]
    #21846948 - 06/23/15 05:53 PM (8 years, 7 months ago)

Quote:

spacechildo said:
if you've succeeded with those containers before just do what you did back then to avoid this.

I suspect there are some other tweaks you've done as well, inoc holes f.ex.




Yea..I need to revisit my steps and really figure out what may have caused the contam. Probably better off going back to starting with agar


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OfflinePsilicon
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Re: Thoughts? [Re: blazedup]
    #21847508 - 06/23/15 08:04 PM (8 years, 7 months ago)

Great reference pic for a nasty cake.  :grin:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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