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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
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Critique my Agar recipe
#21824298 - 06/18/15 04:07 PM (8 years, 7 months ago) |
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I'm just getting into agar and don't want to waste time with weak recipes.
I am about to make a new batch:
400 mL water 8g Agar-Agar 8g BRF (for starch) 8g Karo (for sugar)
Sound good? going for around 2% weight in Agar and a total of 4% starch + sugar. I've read that BRF + Karo works, just wanted to see what people think before I start a PC cycle.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Loc: Milky way
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
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Re: Critique my Agar recipe [Re: bodhisatta]
#21824414 - 06/18/15 04:30 PM (8 years, 7 months ago) |
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Yea I've read through that and PW's agar teks. This is the recipe I've come up with after reading those threads. It sounds good to me in theory, but I have no practical experience with agar.
I guess I can try to do closer to the 6% starch mark
400 mL water 8g Agar-Agar 16g BRF (for starch) 8g Karo (for sugar)
total 6% starch and sugars this way. Does BRF get clumpy when mixing with water like this? I'm not trying to make dough...
Edited by GreenRabbit (06/18/15 04:32 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Critique my Agar recipe [Re: bodhisatta]
#21824419 - 06/18/15 04:31 PM (8 years, 7 months ago) |
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I've done it with just water agar and Karo before. It works. Shoot for a 2% sugar content by weight. 20 g agar agar per liter.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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blackout


Registered: 07/16/00
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Re: Critique my Agar recipe [Re: 36fuckin5]
#21824513 - 06/18/15 04:56 PM (8 years, 7 months ago) |
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I am soon going to be trying out many agar plates looking for pins growing at my low room temp, and making them weak to promote pinning. Workman advises 0.1-0.5% for LCs, his agar was higher but I am not sure if he was looking to fruit on it -or if you are interested. It tends to spread out more on weaker agar so if you have a questionable plate you are transferring from it can be an advantage to be weak.
Quote:
OBTAINING PSILOCYBIN AND PSILOCIN FROM FUNGAL MATERIAL
United States Patent Office 3,183,172
Patented May 11, 1965
Roger Heim, Paris, France, Albert Hofmann, Botimingen, Basel-Land, Arthur Brack, Riehen, near Basel, and Hans Kobel, Basel, Switzerland, and Roger Cailleux, Pavillons-sous-Bois, France, assignors to Sandoz Ltd., Basel, Switzerland, a Swiss firm...
In developing an alternative process which is better adapted for large scale operation, it was found quite unexpectedly that, grown in vitro on substrates rich in nutritive material, the fungi produce active mycelium and sclerotia in large quantities and only a very small number of fruit bodies; on substrates poor in nutrients, however, the familiar fruit bodies are produced. On an agar medium containing 1.5% by weight of agar, a concentration of 0.2 to 0.7% by weight of dry substance of malt extract is the optimum for the formation of fruit bodies, whereas concentrations of 4 to 10% by weight, depending upon the particular fungal species employed, are optimal for the formation of mycelium and sclerotia.
so if looking for stones you might want to go higher.
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GreenRabbit
Plutonium Pollinator



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Re: Critique my Agar recipe [Re: blackout]
#21824542 - 06/18/15 05:01 PM (8 years, 7 months ago) |
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You're trying to get stones to grow on agar? not just the mycelium? Is malt extract your starch?
Well this is for germinating cube spores. I'd like to get an invitro fruit and clone that even.
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blackout


Registered: 07/16/00
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Quote:
GreenRabbit said: You're trying to get stones to grow on agar?
if you are trying to grow spores from MS many will grow out on agar and if they see stones forming they will pick one off and transfer. And people may do the same with cubensis from MS, put a drop on agar and transfer a pin if one grows.
That patent is saying 0.2-0.7% is good for pins, like you are doing.
But if you were trying to get stones 4-10% may be recommended.
But also there is usually faster growth on weaker agar as it goes searching for nutrients. So if you had a dirty print of a stone producing species it might be better to transfer to weak agar at first, so you get a clean transfer and these transfers could be put on stronger agar to see if they produce stones.
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RyeJar
StrangerDanger


Registered: 04/16/15
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Quote:
GreenRabbit said: I'm just getting into agar and don't want to waste time with weak recipes.
I am about to make a new batch:
400 mL water 8g Agar-Agar 8g BRF (for starch) 8g Karo (for sugar)
Sound good? going for around 2% weight in Agar and a total of 4% starch + sugar. I've read that BRF + Karo works, just wanted to see what people think before I start a PC cycle.
You mentioned you don't want to waste time so I'm curious why your not just using the standard tried and true equal parts MEA to agar for a generic easy to make recipe?
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
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Re: Critique my Agar recipe [Re: RyeJar]
#21827749 - 06/19/15 11:20 AM (8 years, 7 months ago) |
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Quote:
RyeJar said: You mentioned you don't want to waste time so I'm curious why your not just using the standard tried and true equal parts MEA to agar for a generic easy to make recipe?
I haven't seen malt extract around yet, not sure where to find it. I don't know if I've seen the tek you are referring to.
From what I've read, 2% sugar, 6% starch in 2% agar is optimal
This is the recipe I ended up making. 400 mL water 8g Agar-Agar 20g BRF (for starch) 8g Karo (for sugar)
Kicked up starch to 5% for a 7% total of sugars and starches. Taking it out of the PC now and inoculating
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Straya
Wot?!

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Quote:
GreenRabbit said:
Quote:
RyeJar said: You mentioned you don't want to waste time so I'm curious why your not just using the standard tried and true equal parts MEA to agar for a generic easy to make recipe?
I haven't seen malt extract around yet, not sure where to find it. I don't know if I've seen the tek you are referring to.
From what I've read, 2% sugar, 6% starch in 2% agar is optimal
This is the recipe I ended up making. 400 mL water 8g Agar-Agar 20g BRF (for starch) 8g Karo (for sugar)
Kicked up starch to 5% for a 7% total of sugars and starches. Taking it out of the PC now and inoculating
I was at my local homebrew shop the other day and you can buy kilos of malt extract there
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RyeJar
StrangerDanger


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Re: Critique my Agar recipe [Re: GreenRabbit] 1
#21832235 - 06/20/15 12:30 PM (8 years, 7 months ago) |
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Quote:
GreenRabbit said:
I haven't seen malt extract around yet, not sure where to find it. I don't know if I've seen the tek you are referring to.
Its not a tek. It's just a standard recipe.
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