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OfflineKindofBlue
The truth is out there


Registered: 10/14/14
Posts: 151
Last seen: 3 years, 6 months
Contam or Weird Pinning???
    #21823903 - 06/18/15 02:31 PM (8 years, 7 months ago)

So this is really weird, but I got these plates from a multi spore, transferred 5 or 6 times before transferring to grain.

Grain growth was very healthy, very white, very fast. Then, after full colonization, I began noticing that every jar was developing a strange brownish coloration at the top. I do not think it is a contam but rather a characterstic of this strain.

However, it seems really weird. Pulled out a plate today and noticed the same thing. It is happening after prolonged sitting so it must be some kind of pinning, but can anyone ID what exactly this might be...?

It happens only after prolonged sitting. And develops on all jars and petris. Never seen it before. Any ideas?



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OfflineKindofBlue
The truth is out there


Registered: 10/14/14
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Last seen: 3 years, 6 months
Re: Contam or Weird Pinning??? [Re: KindofBlue]
    #21824290 - 06/18/15 04:06 PM (8 years, 7 months ago)

anyone?


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OfflineTime_Dilation
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Re: Contam or Weird Pinning??? [Re: KindofBlue]
    #21824344 - 06/18/15 04:16 PM (8 years, 7 months ago)

Looks very similar to blob formation pinning but that brown makes me very nervous. When in doubt....


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OfflineKindofBlue
The truth is out there


Registered: 10/14/14
Posts: 151
Last seen: 3 years, 6 months
Re: Contam or Weird Pinning??? [Re: KindofBlue]
    #21824629 - 06/18/15 05:26 PM (8 years, 7 months ago)

Quote:

KindofBlue said:
anyone?




Besides the "brown" being a generalized contam, do you know specifically what it could be? Is it harmful? Most of this is just for outdoor anyway, but not sure if opening it to spread would cause any harm.


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Offlineblazedup
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Re: Contam or Weird Pinning??? [Re: KindofBlue]
    #21824781 - 06/18/15 05:54 PM (8 years, 7 months ago)

What do you have growing? How long have they been colonizing? The brown in the petri looks like a contam. Most of the time I see brown like that its from metabolites which usually can signal some type of contam. I'm no trusted cultivator but just giving my 2 cents


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Offlineshroomyaxn
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Re: Contam or Weird Pinning??? [Re: blazedup]
    #21825799 - 06/18/15 10:04 PM (8 years, 7 months ago)

"If you see brown, get it out of town."  A shroomery quote I typed down while compiling my notes on contamination. 
Your pictures do not look anywhere near normal, BUT the only contams i have dealt with have been the white blobs of pre-trich forming in my jars towards the end of colonization.  I have not seen a brown contam like that thus far.  :confused:

How big are your gas exchange holes in the tops of those jars?  For mycelium to pin inside a jar would require some sort of increased GE, or maybe the lid is letting in airflow, leading to FAE?

Looks like a mold has taken hold.  :frown:


Edited by shroomyaxn (06/18/15 10:44 PM)


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OfflineMachiavelliavore
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Re: Contam or Weird Pinning??? [Re: shroomyaxn]
    #21826032 - 06/18/15 10:59 PM (8 years, 7 months ago)

Looks like some mold or mold + bacteria contamination to me, though it's strange that it would be ontop in every jar (lids not secure?.)  I would just scrape off the top with a spoon and use it, especially if it's outdoor, unless your space is very limited.  You can see a bit of contam on the side of one of your jars as well.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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