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Invisibleeatyualive
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(Grain Water Liquid Culture) APE Grow Log * 1
    #21820871 - 06/17/15 09:03 PM (8 years, 7 months ago)

:chief:GWLC APE Grow Log:chief:





Brought to you by THE TRIBE


This is a grain water liquid culture growlog using Hamloaf's HLC recipe.

HLC

A 3rd transfer plate from a 2008 APE culture was used to inoculate the GWLC.

A multispore syringe was stored in the fridge from 2008-2014. It was then injected into pf jars. Once colonized the culture was grown out for several runs over the course of the year. A clone specimen was taken. This was injected into 1 pf jar that was stored in the fridge from March 2014 until March 2015. This same jar was then slurried into 50 quarts of grain. Several grains from one of the colonized quarts were placed on agar plates. This is the 3rd transfer pic taken from one plate that is used in the GWLC.



Prep for liquid culture
liquid culture lunch pack from agar/6tango/mycota


cloudy soak water


strained for sediment


before and after coffee filter


after 1:4 dilution as mentioned in HLC tek. held up to light. then regular picture.


wiped the cobwebs off my stirplate! hello old friend.


here is the hamloaf grain water liquid culture. its a few days in. the HWLC was placed on the stir plate twice for about 1 minute bursts on each jar. this was after the spin.



here is the liquid culture after a week. a syringe full was used to inoculate 10 quarts with 1cc per jar. the culture kept on growing and now there is a ton more liquid culture for unlimited amount of jars. It is made a point to stir a few days a week for about a minute each stir for each culture with a nice tornado going.



hello old friend.



The autocleavable Liquid culture syringe tip was used below.  The syringe tip was wrapped in foil. Making sure that the part that opens up is the part that screws on to the syringe body at the bottom of the picture below. The syringe was pressure cooked for 20 mins at 15psi. Once cool, setup a SAB, put all utensils, GWLC, Grain Jars and sterile syringe body still wrapped in the plastic inside the sab.  The syringe is halfway unwrapped so that the tip is not exposed. The foil is unwrapped just enough so that when one can pull the syringe body out, then immediately place the syringe plastic needle on the body. The rest of the foil is covering the syringe tip until the liquid is to be taken.  The lid of the GWLC was lifted, and said LC was sucked into the syringe. Lid was closed. lifted lid of receiving jar, squirted 1cc, then closed the lid of recieving jar. this was one steady motion in a sab. the syringe needle didn't touch anything and this was done for 10 jars in a row. the syringe itself was sterile out of the box. no flame sterilization of the needle was necessary. it worked perfectly sterilizing it in the pc prior to inoculation. careful attention was paid not to touch the syringe needle on anything.

syringe needle


day 2 after inoculation signs of leap off!

Only 1 cc per quart of GWLC was inoculated. all jars are showing leap off. the updated image will more than likely be fully colonized by the time it is checked.



here is 1cc of hamloaf liquid culture without a shake after 12 days. comparatively side by side with a LIquid inoculant of about 15cc of LI per jar after 13 days.

GWLC

LI


here are the GWLC jars ready for spawn


here is my lc after 2 weeks.



2 ape tubs were spawned with ratios below.

APE 1:Uncased: 5 Quarts Spawn: 10 Quarts Strawnet/Coir/dash of gypsum/ 1/2 cup of supercake Substrate:



The OP will be updated as the grow takes place.


--------------------
EAT GETS SHIT DONE


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Edited by eatyualive (06/30/15 06:33 PM)


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InvisibleGrey
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #21820883 - 06/17/15 09:05 PM (8 years, 7 months ago)

Beautiful man. I just put APE to a bunch of masters.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Grey]
    #21820889 - 06/17/15 09:07 PM (8 years, 7 months ago)

oh yeah!

ive got another master and a full pint jar of liquid culture. if only i had some free jars. i am about to g2g 1 master to 16 quarts so i can do some future ape experiments. ill fill you in on the details.


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EAT GETS SHIT DONE


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InvisibleGrey
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #21820895 - 06/17/15 09:09 PM (8 years, 7 months ago)

Sweet. I'm gonna g2g enough to make an outdoor patch. I wanna try it.


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:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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Invisibleshroominmyroom
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Grey]
    #21820965 - 06/17/15 09:28 PM (8 years, 7 months ago)

:flyhigh:


Edited by shroominmyroom (12/12/17 03:45 PM)


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InvisibleNumeroEno
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Grey]
    #21823304 - 06/18/15 11:58 AM (8 years, 7 months ago)

Damn eat! Killin it :rockon:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisiblePastywhyteMDiscord
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: NumeroEno]
    #21823369 - 06/18/15 12:18 PM (8 years, 7 months ago)

:kenthumbup:


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InvisibleBuck513

Registered: 04/17/14
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Pastywhyte]
    #21823595 - 06/18/15 01:18 PM (8 years, 7 months ago)

YES!:rockon:


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Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisible36fuckin5
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Buck513]
    #21823727 - 06/18/15 01:51 PM (8 years, 7 months ago)

So what I'm hearing is that you're about to do an APE giveaway?


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: 36fuckin5]
    #21879487 - 06/30/15 06:30 PM (8 years, 6 months ago)

finally pinned. this cultures on its way out. i waited until the substrate almost began to pin. then threw a 1/4" casing layer composed of coir/verm on top. probably 5-7 early bloomers there. hoping to see something worth swabbing.

nothing special but no stubbies this time. no consolidation ect.



never attempted swabbing these before.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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InvisibleBuck513

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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #21883809 - 07/01/15 05:03 PM (8 years, 6 months ago)

:rockon:


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Buck513]
    #22016853 - 07/29/15 07:49 PM (8 years, 5 months ago)

so i totally forgot about this grow.
2nd flush.




some 4th flush ape from my gwlc!

i kind of forgot about this tub. set it in the corner under the table. found it, blowing up. i have not added water or misted since the dunk after the 2nd flush. i stopped paying attention after the 3rd flush. but today.



--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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OfflinetaGyo
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #22016859 - 07/29/15 07:50 PM (8 years, 5 months ago)

Wish I knew how you guys got your APE so damn nice.


--------------------
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InvisibleGrey
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: taGyo]
    #22016900 - 07/29/15 07:59 PM (8 years, 5 months ago)

:wizard:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: taGyo]
    #22016906 - 07/29/15 08:00 PM (8 years, 5 months ago)

Quote:

taGyo said:
Wish I knew how you guys got your APE so damn nice.



the less i do seems to work better ha.

and tribal dunks. let the sub float in the water on the dunk. don't put the water over the top on the old fruits. the ape and pe end up getting wetspot real easy if you don't evap all the extra water off of the fruits. it can cause the old fruit to turn into some nasty shit.
if you do end up dunking it and get the fruits wet. put a fan on it till the fruits are dry before you put the tub back into fruiting.

the casing has to be very thin. and like azur says, high spawn ratios work much better.



--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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OfflineDarkhome
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #22016929 - 07/29/15 08:05 PM (8 years, 5 months ago)

Great job bro!:billymaythumbup:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
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"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlineblazedup
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: Darkhome]
    #22017002 - 07/29/15 08:18 PM (8 years, 5 months ago)

Nice job..will attempt soon


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: blazedup]
    #22017013 - 07/29/15 08:19 PM (8 years, 5 months ago)

I think im gonna bust out the old lc soon. i have not stirred it in a month :rofl:


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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Offlineblazedup
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: eatyualive]
    #22017019 - 07/29/15 08:21 PM (8 years, 5 months ago)

I need to get my LC work going like that. They really are worth it if you do it right


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Invisibleeatyualive
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Re: (Grain Water Liquid Culture) APE Grow Log [Re: blazedup]
    #22017037 - 07/29/15 08:23 PM (8 years, 5 months ago)

yeah 1 cc per jar for all of the quarts. and there is enough to make hundreds more jars. but don't have any empty right now. ill probably do another few tubs after the PE tubs i have going.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:


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