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ossifrage
Stranger
Registered: 06/16/15
Posts: 15
Last seen: 8 years, 5 months
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Syringe technique, new?
#21820236 - 06/17/15 06:46 PM (8 years, 7 months ago) |
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Thought I would post my syringe making technique. I might post pictures later.
I have several plastic lids to fit regular mouth canning jars, and I have different types (explain later) that I use for spore prints/syringe.
I have combined the spore printing and syringe making in one step. The basic method is to have a self-healing silicone grommet in the lid that you use to inject the water into the jar and remove the water without having to open the jar more than once.
To hold the cap to the lid I have two methods. One, just use plenty of vaseline to stick it to the underside of the lid, the other is to use a wire and vaseline. I attach the cap, inject sterile water, and let sit until I am happy with how many spores have been released. Then I flip the jar over and use the syringe to suck the water and spores back up.
I have modified these by heating the lid and pressing it down on a 1" (2.54 cm) ball bearing. This makes a small well that is lower than the rest of the jar and helps accumulate the spores when sucking the water back up with the syringe.
Ossifrage
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Re: Syringe technique, new? [Re: ossifrage]
#21824555 - 06/18/15 05:05 PM (8 years, 7 months ago) |
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This is a very old technique.
Slight improvement: you could fill the jar partially with water, insert empty needle into the ship, close jar, pc it, then hang a cap by the hook and seal the jar. Aspirate. This way prevents you from having to pierce the ship after pcing.
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ossifrage
Stranger
Registered: 06/16/15
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Last seen: 8 years, 5 months
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Re: Syringe technique, new? [Re: invitro]
#21826870 - 06/19/15 06:18 AM (8 years, 7 months ago) |
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Sorry, my explanation might not have been clear. I do put sterile water in through a seal and leave that sterile syringe in there while the spores are released. In one version I have used a short copper tube to hold the cap up off of the lid. This way when I invert my jar the water does not touch the cap.
I know the method is old, but I have not seen anything with the modifications I did like, the copper tube and creating a small well.
OF
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Gr0wer
always improving


Registered: 09/16/03
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Loc: El Paso, TX
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Re: Syringe technique, new? [Re: ossifrage]
#21843472 - 06/22/15 09:09 PM (8 years, 7 months ago) |
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You could even make the print in an empty 4 or 8 oz jar and store it dry. When your ready to make a syringe shoot sterile water in and suck it up. Your plan sounds solid, you can even fill multiple syringes. Not sure if others have done this before though.
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ossifrage
Stranger
Registered: 06/16/15
Posts: 15
Last seen: 8 years, 5 months
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Re: Syringe technique, new? [Re: Gr0wer]
#21861999 - 06/26/15 10:52 PM (8 years, 7 months ago) |
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Me either, the only reason I bothered to post it. I know the jar method is old, but I haven't read anything about similar modifications. I'm a bit of a greedy person in a particular way. I am a scientist so we are always about getting the most out of something due to budget constraints. Medical and scientific equipment is the biggest rip-off in the world. Not to mention most universities take 50 to 60% of grant monies for "administrative costs". This means if you know it will take X USD you still have to ask for at least twice that amount, at best, assuming no award reductions from the funding party.
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Obsidian
The lone deranger



Registered: 06/13/14
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Re: Syringe technique, new? [Re: ossifrage]
#21886593 - 07/02/15 07:04 AM (8 years, 6 months ago) |
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I wanted to do that, but then I realized one thing - when you have a jar with some water which you put on room temperature - water vapor will condensate on the lid and drip onto the cap, picking all the contaminants from the cap and taking them down into your spore water. So I would prefer the dry version of the idea.
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ossifrage
Stranger
Registered: 06/16/15
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Last seen: 8 years, 5 months
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Re: Syringe technique, new? [Re: Obsidian]
#22055359 - 08/06/15 08:09 PM (8 years, 5 months ago) |
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Plenty of ways around that. You can create things to channel the water away from the cap or cover the cap in any sterile protector to prevent such things. Also, unless you get bacteria, it is unlikely that you would have enough non-target spores to out compete target spores when germinating.
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Syringe technique, new? [Re: ossifrage]
#22059473 - 08/07/15 04:33 PM (8 years, 5 months ago) |
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What about all the bacteria on the spores? In typical spore printing methods most of the bacteria are killed off by letting the print sit and dry out before use but you're skipping that step.
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