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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185489 - 12/17/03 02:21 PM (20 years, 1 month ago)

Maybe if you were a bit constructive this conversation could go somewhere. Honestly, I'm getting sick of you posting insults to this board (this is the second one in addition to the one you edited or deleted) to people who know way more than you about the subject.

I answered your question, already -- why don't you try round-up like I suggested? I'm sure, though, that you already knew glyphosate inhibited 5-enolpyruvylshikimate-3-phosphate synthase and I'm sure you also came to the conclusion, based on your knowledge of enzymatic pathways, that this would inhibit psilocybin production, right off the top of your head, too.

No, I guess I'm just not that smart.

When you ask a question, I'll answer it, like I did here. If you want me to explain my response please ask me to, but quit insulting people on this board who are trying to help.

--
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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185522 - 12/17/03 02:38 PM (20 years, 1 month ago)

BTW -- if you add roundup you may need additional supplements in the media if it can no longer produce them in situ. I don't know if round-up would kill the fungus without supplements or not.

--
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OfflineBaby_Hitler
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185609 - 12/17/03 03:20 PM (20 years, 1 month ago)

Actually, I don't trust your "information" so please do not respond to my threads in the future.

Thanks.


--------------------
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OfflineBaby_Hitler
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185627 - 12/17/03 03:30 PM (20 years, 1 month ago)

Quote:

micro said:
BTW -- there would be 2 copies of the gene, anyway, unless it's only a monokaryote we're talking about.

--
Micro





Are you suggesting that psilocybin production is regulated by one gene? If you can site a reference for that that I can check, I will give you due credit.


--------------------
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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185770 - 12/17/03 04:34 PM (20 years, 1 month ago)

Quote:

Actually, I don't trust your "information" so please do not respond to my threads in the future.




Aren't you the one who said you could sequence DNA with an $80 childrens' toy? Wowwww.... You must be a genius, there, kiddo.... Especially in the field of genetics. If anyone would like to see this person make an ass out of theirself, please read this thread:

http://www.shroomery.org/forums/showflat...;o=&fpart=1

Quote:

Are you suggesting that psilocybin production is regulated by one gene? If you can site a reference for that that I can check, I will give you due credit.




Yes, I am. Things are controlled by one gene. There is one gene for each enzyme. You can have multiple copies of the RNA made, but it's controlled by one gene.

It's not multi-allelic because the genes code for enzymes.

I guess you don't have to believe me, but you obviously have proven that you know nothing about genetics. I don't see how you can argue with someone who works in microbiology about microbiology -- you want to play? I'll make your fucking head spin -- I can prove you know nothing because you don't.

Of course, there are many people like you in this world, who think they're the expert on subjects which they haven't a fucking clue, but you don't realize that by being ignorant you're just less of a person.

If you want serious discussion I'm all about it, but I'm not a second-grader, and I don't think like a second grader.

Happy trails you fucking baby....


hitler

--
Micro


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Edited by micro (12/17/03 04:50 PM)


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OfflineBaby_Hitler
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185811 - 12/17/03 04:52 PM (20 years, 1 month ago)

Quote:

I don't see how you can argue with someone who works in microbiology about microbiology -- you want to play? I'll make your fucking head spin -- I can prove you know nothing because you don't.





I don't see how you can argue with a person who works in a castle on the moon. Why should I or anyone believe you?

You can make up facts you can't prove all day to try and make someone's "head spin". You can prove you know things, (the flushable toilet was invented by Thomas Crapper) but can you prove that those things mean what you say they mean?

You still haven't proven that there is a single gene that regulates the amount of psilocybin produced.


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Invisibleeric_the_redS
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185837 - 12/17/03 05:00 PM (20 years, 1 month ago)

Quote:

Baby_Hitler said:
You still haven't proven that there is a single gene that regulates the amount of psilocybin produced.




and nobody will be able to prove, or disprove, it until extensive research is done.

bring on the experiments.


--------------------
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Re: A legal cultivar of cubensis possible? [Re: eric_the_red]
    #2185843 - 12/17/03 05:02 PM (20 years, 1 month ago)

You sir have a heart the size of an elephant's testicles! :wink: :thumbup: :stoned: :thumbup:


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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185850 - 12/17/03 05:06 PM (20 years, 1 month ago)

Quote:

You still haven't proven that there is a single gene that regulates the amount of psilocybin produced.




Find me some multiple copies of genes encoding enzymes on the Shikimic Acid Pathway:

http://www.blackwell-synergy.com/links/doi/10.1046/j.1432-1327.1999.00244.x/full/

....if you can read and understand it. Sometimes a locus will duplicate, but that's rare, especially in this case -- it's almost always one gene on one locus.

--
Micro


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Invisibleeric_the_redS
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185853 - 12/17/03 05:07 PM (20 years, 1 month ago)

what you didn't know is that i also have testicles the size of an elephant's. i have a seat everywhere i go.

i really do wish there were more resources (funds, labs, people) available to research shrooms. until laws change though, i don't see that happening.  :sad:


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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185867 - 12/17/03 05:12 PM (20 years, 1 month ago)

http://www.ncbi.nlm.nih.gov/entrez/query...p;dopt=Abstract

http://www.ncbi.nlm.nih.gov/entrez/query...p;dopt=Abstract

http://www.ncbi.nlm.nih.gov/entrez/query...p;dopt=Abstract

....do a search for more, if you want. You won't find anything, except maybe some duplications, maybe, but that's pretty much it. When that happens, though, both of them work, anyway.

--
Micro


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Invisiblemicro
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Re: A legal cultivar of cubensis possible? [Re: eric_the_red]
    #2185886 - 12/17/03 05:20 PM (20 years, 1 month ago)

Quote:

what you didn't know is that i also have testicles the size of an elephant's.




http://elephantiasis.freeyellow.com/

--
Micro


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OfflineBaby_Hitler
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185887 - 12/17/03 05:20 PM (20 years, 1 month ago)

"Ergot alkaloids are synthesised from several pathways. "

http://bugs.bio.usyd.edu.au/Mycology/feed/SecondaryMetabolites/SecMetabText.htm

Ergot alkaloids are synthesised from several different pathways (which would definitely mean more than one gene influences production) On what do you base your assumption that this cannot be true for psilocybin?


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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2185919 - 12/17/03 05:32 PM (20 years, 1 month ago)

I can see how your theory that glyphosate could inhibit psilocybin production is plausable, but unfortunately, the only method I have to determine potency is to eat the mushroom.

glyphosate is too toxic to allow me to do that, however, if it could be proven to work reliably there would be no reason to eat mushrooms grown with it. Unfortunately it may have such an impact on the health of the fungus that the result would still be unusable.

But it is a possibility.


--------------------
Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ
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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2185925 - 12/17/03 05:35 PM (20 years, 1 month ago)

A pathway is not an enzyme. Like I said above -- each enzyme is encoded by a single gene.

There aren't many copies of each gene, so you can't breed a mushroom to not produce psilocybin (or produce it in extremely minute quantities.) You need to have a mutation on the pathway.

I didn't say that the whole shikimic acid pathway, tryptophan pathway and psilocybin pathway is encoded by one gene, it's a bit obvious that it's not....

Honestly, you're boring the shit out of me so I'm going offline to smoke a bowl. I'll be back on tomorrow just incase anyone has anything interesting to say.

--
Micro


Edited by micro (12/17/03 05:49 PM)


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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2186016 - 12/17/03 06:16 PM (20 years, 1 month ago)

Micro got Baby Hitler 0wn3nd. I stand by what Micro says. He knows his shit. So dont question what he states.

That is all. Carry on.


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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2186024 - 12/17/03 06:19 PM (20 years, 1 month ago)

Quote:

micro said:
Quote:

what you didn't know is that i also have testicles the size of an elephant's.




http://elephantiasis.freeyellow.com/eleph-fiji.gif



elephantitas of the balls. lol


Edited by Paul808996 (12/17/03 06:22 PM)


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OfflineBaby_Hitler
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Re: A legal cultivar of cubensis possible? [Re: micro]
    #2186174 - 12/17/03 07:07 PM (20 years, 1 month ago)

Also your magic scientist powers have overlooked the possibility that there may be some genes that produce enzymes that may inhibit or disrupt production of psilocybin at different stages.

Maybe tomorrow we'll get into the fact that you have suggested that:

1.) there is only one gene that influences production of psilocybin.

2.) this gene is "not multi-allelic", which would mean that all cubensis mushrooms have the same allele for production of psilocybin.

This would mean that there can be no variation in potency due to genetics. Last I recall, popular opinion was that this is not true.


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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2186351 - 12/17/03 08:11 PM (20 years, 1 month ago)

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Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
    #2186357 - 12/17/03 08:16 PM (20 years, 1 month ago)

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