|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
YidakiMan
Stranger

Registered: 09/28/02
Posts: 2,023
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2182312 - 12/16/03 11:38 AM (20 years, 1 month ago) |
|
|
Perhaps something like this would answer the question; why do mushrooms produce psilocybin and psilocin?
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: YidakiMan]
#2182328 - 12/16/03 11:42 AM (20 years, 1 month ago) |
|
|
That question had been asked a million times.
I don't think anybody knows yet.
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
zbgeed
Journeyman
Registered: 07/25/03
Posts: 95
Loc: My Hideout
Last seen: 19 years, 3 months
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2182420 - 12/16/03 12:12 PM (20 years, 1 month ago) |
|
|
i understand wher you're coming from, and i agree. research on these mushrooms would be a wonderful thing. But i totally disagree with trying to genetically alter them. If you want to study them then take the risk. Its for the sake of science, right?
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: zbgeed]
#2182433 - 12/16/03 12:19 PM (20 years, 1 month ago) |
|
|
Who said anything about genetically altering anything!?
I'm talking about finding one mushroom that contains zero psilocybin, and cloning it, and distributing live cultures of it.
That's all.
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
Granola
bag lady

Registered: 05/18/03
Posts: 411
Loc: 50.0N-6.0E
Last seen: 18 years, 4 months
|
Re: A legal cultivar of cubensis possible? [Re: YesItsMe]
#2182717 - 12/16/03 01:54 PM (20 years, 1 month ago) |
|
|
Quote:
YesItsMe said: I find cubensis very decorative , so it could be an issue to legal cultivation .Poppies are legally cultivate in garden , so why not this fucking shrooms ?!!! Laws must be respect , but we should respect people who learn by themself !
Poppies are not legal for cultivation in the US with out permits and for those your name needs to be Pfizer or Rhorer, DEA see a poppy in your yard they take you to jail
|
Anonymous
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2183207 - 12/16/03 04:19 PM (20 years, 1 month ago) |
|
|
I don't think there is any substrain of cubensis that doesn't produce the goodies.
You would have to genetically modify the mushroom to make a key enzyme unavaliable, so no goodies are produced.
That would probably be transfered to spores. Potentially it could harm wild cubensis populations.
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: ]
#2183242 - 12/16/03 04:34 PM (20 years, 1 month ago) |
|
|
It may not matter unless there is precicely zero illegal chemicals in it. Hemp contains a few Parts Per Million of THC, but is still legal.
Cubensis though, I don't think the law would allow even one average dose in a pound of dried fruitbodies. It would have to be perfectly and completely incapable of producing actives.
That seems likely to require some actual genetic modification. The only method I can think of that would be practical to use would just be simple selection. It would not be very practical to try selective pressure, or any breeding techniques.
Selection alone may be able to do it, but it may require testing millions of fruitbodies.
I wish search were working right now. I know I've seen people report mushrooms that did absolutely nothing even in large quantity.
Seems like PR's were particularly capable of producing duds.
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: ]
#2183246 - 12/16/03 04:36 PM (20 years, 1 month ago) |
|
|
Can you think of any additives that could be used in the substrate that would prevent production of socially unacceptable molecules?
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
Anonymous
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2183681 - 12/16/03 07:13 PM (20 years, 1 month ago) |
|
|
Processed hemp contains very little , but the living plant contains enough that it remains illegal in the usa. You can't get high on the stuff, but it is still illegal.
I have never found a dud. I guess if you did some serious single cell cultures, you might find a cell that doesn't have the ability, and then grow out cultures from that single cell.
|
enimatpyrt
addict
Registered: 11/05/03
Posts: 498
Last seen: 20 years, 29 days
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2183711 - 12/16/03 07:25 PM (20 years, 1 month ago) |
|
|
Quote:
Baby_Hitler said: This is primarily about American law, but also Canadian, and international law...
Would it be possible to isolate a clone of cubensis that produced absolutely no illegal substances?
If so then (in America as I understand the law) this clone could be legally circulated, and cultivated. This would, I believe, furthur the science of Psilocybe cubensis cultivation greatly. Many of our most talented people are unable to participate in the cubensical sciences because they decided that the legal risks were too great.
I think if you think about it for a moment, all kinds of benefits can be imagined that could be gained from the existance of a legal cultivar of cubensis.
Anyone care to guess how this might be done, and how low a psilocybin content is even possible in a cubensis?
Maybe we could breed a no-THC content marijuana and spends lotsof money and time learning to grow it too! And mescaline-less peyote!
Or, you could grow any of the other tens of thousands of types of non-illegal, sporulating, mushrooms in existance. You could grow a number of edible mushrooms, I'm not sure which specific genus / species of mushrooms would work, that reproduce and grow like p. cubensis
|
micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2184126 - 12/16/03 10:19 PM (20 years, 1 month ago) |
|
|
Quote:
Even if such a cultivar were isolated, it's spores would still produce mushrooms that produce psilocybin.
Not really. If you knocked out the gene and it can reproduce the offspring wouldn't produce it, either, unless it mated with a fungus that was active.... That's not the problem -- the problem is that noone cares. If you want LBMs for asthetic value grow non active LBMs. Noone will care enough to study non-active psilocybe mushrooms. If there was any reaon to study active species it can be done, but noone does because noone cares enough to offer a grant. Unless there's some monetary value involved in the research or some interest by the benefactor there's not going to be any money put into the research.... -- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (12/16/03 10:22 PM)
|
zeronio
Stranger


Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 years, 4 months
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2184591 - 12/17/03 02:53 AM (20 years, 1 month ago) |
|
|
Quote:
why do mushrooms produce psilocybin and psilocin?
Quote:
That question had been asked a million times.
I don't think anybody knows yet.
There is a simple explanation. Psilocybin gene had an advantage in evolution because the mammals helped mushrooms that had it to spread their spores. They formed a symbiotic relationship with mammals.
(/me puts a helmet on in anticipation of anti-evolutionists counter strike )
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: micro]
#2184808 - 12/17/03 08:02 AM (20 years, 1 month ago) |
|
|
All of that would depend on how it was done, which will depend on how it can be done. If certain permutations of alleles have this effect, and an individual could be found with one of these combinations, then it's spore offspring would posses all of the alleles needed to produce the chemo/phenotype, but only a few would consist of a non-active permutation of those alleles. In other words I'm looking for a natural born mushroom with this characteristic. It may or may not be out there somewhere, but if it is I'll find it, and when I find it youll see... YOU'LL ALL SEE!!!!!!!!!!!!!!!!1111 [rubs hands maniacally]
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
Edited by Baby_Hitler (12/17/03 08:21 AM)
|
micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2185206 - 12/17/03 12:01 PM (20 years, 1 month ago) |
|
|
?syntax error.
Permutaion = mutation? If something's DNA is mutated then it will transcribe it to the offspring if it can reproduce. The chances of randomly gaining the ability back are even less (to a great extent) because a point mutation or whatever can happen in a lot of places and mess up the pathway but the chances of the random mutating of that particular spot back to what it was is pretty much null.
You might be able to inhibit the production of psilocybin with something like glyphosate, though, or RNAi....
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
G a n j a
Pictish and proud


Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
|
Re: A legal cultivar of cubensis possible? [Re: micro]
#2185216 - 12/17/03 12:10 PM (20 years, 1 month ago) |
|
|
Wouldnt glyphosate affect the taking up of water and its movement inside the mushroom, as it does in plants? Making it dry out and die?
-------------------- er
|
micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: A legal cultivar of cubensis possible? [Re: micro]
#2185221 - 12/17/03 12:11 PM (20 years, 1 month ago) |
|
|
BTW -- there would be 2 copies of the gene, anyway, unless it's only a monokaryote we're talking about.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: A legal cultivar of cubensis possible? [Re: G a n j a]
#2185225 - 12/17/03 12:16 PM (20 years, 1 month ago) |
|
|
Quote:
Wouldnt glyphosate affect the taking up of water and its movement inside the mushroom, as it does in plants? Making it dry out and die?
Glyphosate is an inhibitor of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in the shikimic acid pathway.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: micro]
#2185330 - 12/17/03 01:19 PM (20 years, 1 month ago) |
|
|
No, we're not talking about any genetic mutation at all. Permutation = combination in a particular order.
123 231 312 321 213 132 are all different permutations of the same combination.
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
micro
bunbun has a gungun


Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: A legal cultivar of cubensis possible? [Re: Baby_Hitler]
#2185340 - 12/17/03 01:24 PM (20 years, 1 month ago) |
|
|
It's a mushroom, not a combination lock.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
Baby_Hitler
Errorist



Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 1 hour, 15 minutes
|
Re: A legal cultivar of cubensis possible? [Re: micro]
#2185393 - 12/17/03 01:46 PM (20 years, 1 month ago) |
|
|
I apologize if first year college algebra is over your head.
I'm surprised that you fail to see how this applies to genetics, Perhaps your claims of knowledge are overstated.
-------------------- Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ (•_•) <) )~ ANTIFA / \ \(•_•) ( (> SUPER / \ (•_•) <) )> SOLDIERS / \
|
|