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Invisiblemeowshroom
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Registered: 04/28/15
Posts: 158
OK to use NaCl 0.9% sterile syringes to store spores?
    #21817166 - 06/16/15 11:16 PM (8 years, 7 months ago)

Was wondering, if after taking a print, if one could just use a medical sterile saline solution to drop a few sterile drops onto the print, mix it around with the tip of the sterile syringe, and then suck it all back up, and cap off with sterile tip.

Would the NaCl at 0.9% harm the spores?    I know using regular DI or RO water is the obvious choice, but if one wants to skip that step of contamination, would this be a possibility, or?


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OfflineDensePlacebo
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Registered: 05/14/15
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Re: OK to use NaCl 0.9% sterile syringes to store spores? [Re: meowshroom]
    #21817192 - 06/16/15 11:27 PM (8 years, 7 months ago)

I'm pretty sure salt is bad


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Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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OfflineShroominMe
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Registered: 05/03/15
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Last seen: 8 years, 3 months
Re: OK to use NaCl 0.9% sterile syringes to store spores? [Re: DensePlacebo]
    #21817253 - 06/16/15 11:47 PM (8 years, 7 months ago)

Quote:

Doc_T said:
Quote:

jesson85 said:
after i add a small fragment of mycelium to the saline solution contained in a test tube, should i incubate it for a period of time?




NOT saline! Salt will kill your fungus.
Sterile water.




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Invisiblemeowshroom
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Re: OK to use NaCl 0.9% sterile syringes to store spores? [Re: ShroominMe]
    #21817309 - 06/17/15 12:11 AM (8 years, 7 months ago)

Thanks for confirming what I thought.  It was worth asking since it's an easily obtainable AND STERILE piece of kit.

I know most bacteria, etc don't like salt, but in 0.9% concentrate, it's so low, that I was wondering if it would kill bacteria but not spores.  Kind of an advanced question that one would have to test...  :evilgrin:


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OfflineShroominMe
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Registered: 05/03/15
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Re: OK to use NaCl 0.9% sterile syringes to store spores? [Re: meowshroom]
    #21817331 - 06/17/15 12:22 AM (8 years, 7 months ago)

Quote:

meowshroom said:
  Kind of an advanced question that one would have to test...  :evilgrin:



I agree but I don't do enough grows to be in a position to do any experiments. Let us know what you find if you do decide to test! :wink:


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