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Offlinemushpunx
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Putting plates from cold storage to grain
    #21814153 - 06/16/15 10:57 AM (8 years, 7 months ago)

Hey all

  So I have a few clone plates in the fridge I forgot I had grown out to put to master jars. Theyre not touching the walls of the plates or anything but pretty grown out -I had these ready to go to grain I just didnt have the space so I stored them.

Its been a couple months, just wondering is it bad practice to put these plates to grain or should I grow new plates first?

  Thanks!

-Mushpunx


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Invisiblemaddchef
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Re: Putting plates from cold storage to grain [Re: mushpunx]
    #21814167 - 06/16/15 11:00 AM (8 years, 7 months ago)

That's a personal call. Half of us will transfer to a new plate first and half will just use them.


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InvisibleNumeroEno
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Re: Putting plates from cold storage to grain [Re: maddchef]
    #21814195 - 06/16/15 11:13 AM (8 years, 7 months ago)

I like to transfer to a new plate with a different media to get it hungry again. A little while ago, I used a 3 month old MEA PE plate to inoculate a grain water agar plate. The transfer took over the grain water agar plate extremely fast, and now it's doing really well on the grain.


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InvisiblePirateSwazey
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Re: Putting plates from cold storage to grain [Re: NumeroEno]
    #21814521 - 06/16/15 01:04 PM (8 years, 7 months ago)

I always transfer to a new plate.. Sometimes they will sit in there for 4 or 5 months & I don't trust parafilm for that long!


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Offlinemushpunx
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Re: Putting plates from cold storage to grain [Re: PirateSwazey]
    #21815040 - 06/16/15 03:06 PM (8 years, 7 months ago)

Thanks guys yea Im glad its not just me getting weirded out. Refridgeraters are dirty who knows whats crawling around in there :lol:

I have a dedicated mini fridge for plates and slants but its still in the garage so my stacks of plates are in my (icky) fridge.
I dunno theyre prolly clean but I would hate myself to lose a grow from trying to save a few days time right


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Invisible36fuckin5
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Re: Putting plates from cold storage to grain [Re: mushpunx]
    #21815476 - 06/16/15 04:34 PM (8 years, 7 months ago)

You got it exactly right. Make a transfer to be sure it's all good. Much better to waste 3-4 days waiting on a petri than 1-2 weeks waiting on a grain jar that might not work out.


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i recommend common sense and figuring it out.

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