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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Where to grab from
    #21813717 - 06/16/15 08:14 AM (8 years, 7 months ago)

I am attempting to isolate clean myc from a horrid contam.
I will let the community tell me where to pull from


And this is likely the contam that I'm running from

I can't tell what is what anymore

So please if you're unsure leave it be.

For reference this is what it looks like meshed with mushroom myc



--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/16/15 08:18 AM)


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OfflineSksoul
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Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Where to grab from [Re: maddchef]
    #21813892 - 06/16/15 09:28 AM (8 years, 7 months ago)

in that bottom pic, 4-8 oclock looks okay and a piece at 9 oclock. get yourself 2 different paths to take.

Take a smallpiece as I know you've been trying to get ahead of these contams for a while.

I can't see shit in your first 2 photos. its focused on the dish, not the mycelium.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: Sksoul]
    #21813922 - 06/16/15 09:39 AM (8 years, 7 months ago)

The third dish isn't in play.

I can't get a better pic sadly as my phone either will focus on the dish or the light source. The myc is still very thin


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineSksoul
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Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Where to grab from [Re: maddchef]
    #21813950 - 06/16/15 09:45 AM (8 years, 7 months ago)

cant see the first picture clearly. from what I can see of the second picture, the top half of the petri looks good from 11-1.

the bottom looks like it has that bacterial halo around the entire culture... maybe just thin wispy mycelium like you said


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: Sksoul]
    #21813971 - 06/16/15 09:51 AM (8 years, 7 months ago)

It's def not bacteria.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineSksoul
Pan handler
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Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Where to grab from [Re: maddchef]
    #21814003 - 06/16/15 10:03 AM (8 years, 7 months ago)

good luck, make a bunch of cultures from grabs around the entirety of growth. see what happens. 1-3 o'clock, 6, 9, and 10 o'clock look like they've separated a bit into strong/clean growth in the bottom half of the petri


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


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InvisiblePirateSwazey
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Re: Where to grab from [Re: Sksoul]
    #21814530 - 06/16/15 01:06 PM (8 years, 7 months ago)

1st two plates look good, 3rd is shot, 4th is ok. The clear growth you're seeing is cube myc.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: PirateSwazey]
    #21814538 - 06/16/15 01:09 PM (8 years, 7 months ago)

Plate 4 from 11-3 is mold is it not?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePirateSwazey
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Posts: 2,993
Loc: Here, Now
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Re: Where to grab from [Re: maddchef]
    #21814557 - 06/16/15 01:14 PM (8 years, 7 months ago)

Nope. I learned that over the winter. I isolated it over a few months and actually came out with a banging isolate I dubbed the invisible woman..

http://www.shroomery.org/forums/showflat.php/Number/20930437

Transfer it a few times & see for yourself :wink:


I stopped seeing it when I started using a little less agar mix (I use pre-mixed powder, so less nutrient) in my recipe.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: PirateSwazey]
    #21814564 - 06/16/15 01:16 PM (8 years, 7 months ago)

Odd. I still don't trust it considering the similar appearance of what I'm running from.

And this is on half strength nutrient plates. Hoping to get rhizo growth more readily.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePirateSwazey
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Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Where to grab from [Re: maddchef]
    #21814656 - 06/16/15 01:33 PM (8 years, 7 months ago)

Here is the best advice I have been given about shit like what you're going thru...

Quote:

The shroomy 1 said:
I've never seen it in any of my plates, but If I were you I would Isolate it and grow it out on it's own plate.  Grow it out and study it.  If and when it colonizes a plate, then transfer it to it's own jar of grain.  See how it grows and notate all of it's characteristics.  Get to know your enemy.  :evil:  That is how you become knowledgeable in this field.




That guy is an old hand & a grower I respect!

Also you might be seeing it by using lower nutrient as the myc is spreading itself so thin like that.

One thing I didn't mention in that thread was at the time I was using that stupid agar slurry tek a lot, and I'm pretty sure that's where my contams were coming from. Other growers that I trust do not endorse that technique either :thumbdown:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: PirateSwazey]
    #21814679 - 06/16/15 01:38 PM (8 years, 7 months ago)

I did isolate it. Third plate is it by itself. It it clearly distinguished alone.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Where to grab from [Re: maddchef]
    #21814687 - 06/16/15 01:40 PM (8 years, 7 months ago)

I did not however put it alone into grains.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
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