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Offlinejong21
Mycologist/CSMajor

Registered: 05/28/03
Posts: 576
Loc: Berkeley, CA
Last seen: 13 years, 2 months
What am I doing wrong
    #2181299 - 12/16/03 01:02 AM (14 years, 4 months ago)

I was hoping the purchase of my pressure cooker would help reduce contams. However, on my last 12 jars, I had 3 contams....when I boiled I had 4 the last two times.

I did 6 cam and 6 blue meanie...all 6 blue meanie are fine, 3/6 cams got this stuff that started out as a widespread thin whitish gray fluff, and quickly turned into a deeper coarser white, definitely not mycelium, that took over the entire jar at once.

I flamed the needles before I innoculated, used torch lighter got them bright red.

I have a 1/2 inch layer of dry mycelium covering the substrate, with 1 piece of tin foil to cover up the lid/4 holes.

I never used tape....should I use tape to cover up the holes at first? I know the mycelium will colonize faster with some air., exchange, but shoudl I wait a few weeks before uncovering the holes?

I am just wondering what the possible causes of the problem could be. My only guess is that something got on my cambodian syringe after I innoculated the first few. I didn't flame after each innoculation. This is my only guess.


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OfflineKaren
Pooh-Bah
Female

Registered: 01/03/00
Posts: 1,677
Last seen: 10 months, 27 days
Re: What am I doing wrong [Re: jong21]
    #2181320 - 12/16/03 01:08 AM (14 years, 4 months ago)

Quote:

I didn't flame after each innoculation.




That could very well do it! Do that next time and see if your contam rates improve.


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Offlinejong21
Mycologist/CSMajor

Registered: 05/28/03
Posts: 576
Loc: Berkeley, CA
Last seen: 13 years, 2 months
Re: What am I doing wrong [Re: Karen]
    #2181365 - 12/16/03 01:34 AM (14 years, 4 months ago)

ok thanks....hehe I thought I could get away with it  :rolleyes:


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I either talk about my friends in the first or third person, but I never, ever talk about myself on this website. Except that last sentence.


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InvisibleDatDaNK420
SeriousShroomage
Registered: 06/23/03
Posts: 612
Re: What am I doing wrong [Re: jong21]
    #2181606 - 12/16/03 04:26 AM (14 years, 4 months ago)

Well I've personally never used a PC. I use a nice big pot and boil them on low for an hour and a half with the lid on the pot. I've never had a jar contaminate on me yet. I flame the needle first and then wipe it with rubbing alcohol in between jars.


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Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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