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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Ghetto grow. Slurried PF cakes to untreated coir -- It worked! And then it triched out.
#21812103 - 06/15/15 07:49 PM (8 years, 7 months ago) |
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I'm copying this from my journal since it actually produced mushrooms 
So, a little backstory. I'm always trying to figure out how to simplify things, and while I prefer to cultivate using professional methods, I always like to know how easily something can be done. This thread explores what is IMO the simplest, cheapest way to cultivate a decent supply of mushrooms. I took pics of the whole process, and I will flesh this out if things go well, but here's what we're starting with. If you're using a vendor syringe this process can be done without the use of a pressure cooker, still air box, or pasteurization.
I did not use a vendor syringe, so a still air box was used to make a spore syringe. A foil wrapped shot glass and a tall half pint jar with a modified plastic lid was filled with tap water, and boiled in a pot for 45 minutes. Inside the still air box, still hot water was drawn up into a fresh, factory sterilized syringe and needle, and allowed to cool. The shot glass was unwrapped, and water was squirted from the syringe over a print of lizard king courtesy of carc. The spore water was drawn back up, and the syringe was capped until ready for use.
I prepared 6 standard PF jars and boiled for 2 hours. The next day, the 6 PF jars were inoculated with the spore syringe in still, open air. The needle was flamed in between each jar, not each inoculation.
5 out of 6 jars colonized completely. One was lost to trichoderma. Pretty average for open air.
One jar pinned invitro, so I dunked and rolled it and put it in a mini SGFC that I had running for another project. I could have used it for the slurry, since pins make great inoculant.
The PF cakes were sliced up, and blended in a freshly washed oster blender pitcher.
The slurry was poured directly on to the coir and verm, and water was added until field capacity was reached and the slurry was evenly distributed.
Here we are at this point. One 15 quart mini mono was filled with the substrate.
Now we wait.

48 hours after spawning.

72 hours after spawning, the tub is fully colonized.

I added a very thin casing layer of untreated coir/verm and put the tub into fruiting conditions. I'm cautiously optimistic.

Update 6/12/15!!!
Just shy of 11 days after spawning the tub, the first pins are popping up.

Update 6/14/15
My concerns about the casing layer triching out were spot on. I woke up to this. I cut the trich out. There are some nice looking pins, but this tub is only going one flush.

Update 6/15/15
Picked the first mushrooms from the ghetto tub today. Got a nice cluster. The rest of the first flush is coming in nicely, but so is the trich. Definitely tossing this after I harvest the rest of the flush.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (06/15/15 07:53 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Ghetto grow. Slurried PF cakes to untreated coir -- It worked! And then it triched out. [Re: NumeroEno]
#21812229 - 06/15/15 08:22 PM (8 years, 7 months ago) |
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trich is a facultative parasite of cubensis. when given opporunty it acts as it does on other fungi. trich has chitinases so it naturally attacks other fungi made of chitin. by the time you see green it's grown in, that's why we don't find little nice pockets of trich in a substrate. and that's why good luck cutting it out.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Ghetto grow. Slurried PF cakes to untreated coir -- It worked! And then it triched out. [Re: bodhisatta]
#21812293 - 06/15/15 08:34 PM (8 years, 7 months ago) |
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Yep, and that is really the bottom line here. No, you do not need to heat treat coir to get a flush, but that trich is gonna take over eventually if you don't kill the spores.
Anyway, this thread is more about the slurried PF cake mini mono. IMO this is a lot better way to do a super basic grow than fruiting cakes in a sgfc.
I'm starting some new PF cakes for a second slurry tub, but these PF cakes will be started with LI and the bulk substrate will be properly pasteurized. I'm gonna find out exactly how much 4 slurried PF cakes spawned to a mini mono will yield.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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