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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21829489 - 06/19/15 07:01 PM (8 years, 7 months ago) |
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Where am I putting these jars after inoc cold or warm?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21829772 - 06/19/15 08:00 PM (8 years, 7 months ago) |
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Room temp is fine. 72 is ideal. Don't incubate to warm because mold and bacterial thrive in warmer environments
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: PinPornProducer]
#21829821 - 06/19/15 08:10 PM (8 years, 7 months ago) |
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Quote:
PinPornProducer said: 45 minutes in a pc is what I did for pf jars, I've never steam sterilzed so I can't really say for sure but I think they only need an hour or so but 3hrs won't hurt anything so that should be fine.
agree it doesnt hurt, just wanted to add for the records that TEKs says 90mins steaming pf-tek in a pot and 60mins @ 15 psi for pf-tek. not for grains, pf-tek only!
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shroomyaxn



Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: spacechildo]
#21830243 - 06/19/15 09:42 PM (8 years, 7 months ago) |
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Some advice for your SAB: -wearing those thicker latex cleaning gloves while doing sterile work can be difficult. maybe not when inoculating with a single syringe, but when doing G2G or agar work it can become cumbersome. -use the tighter fitting medical style gloves with a fresh pair of tyvek sleeves that come up to your biceps. -use 70% iso alc, not the 90% or 50%. -investing in an alcohol squeeze bottle is a smart idea. you can use it to to easily re-apply the 70% iso on your hands while working in the SAB -the plastic secured from the SAB to your gloves is not necessary, it will eventually start to harbor contams. you could wipe the plastic down with 70% iso every time you use your SAB, but in my opinion it gets in the way. -an SAB does not need to be air tight. a slight space between your arms and the SAB holes is okay. just move smoothly in your SAB and try to not stir up air while doing your sterile work.
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: shroomyaxn]
#21830495 - 06/19/15 10:49 PM (8 years, 7 months ago) |
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I'm done! Got em all knocked up. I might have put to much spore solution in I put two of the black ticks maybe a lil more in each hole. Hope they will b ok. Got 4 b+ ,3gt & 3 Eq. They are now in my closet which has no door so there's natural light will that be ok?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21830505 - 06/19/15 10:53 PM (8 years, 7 months ago) |
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Perfect
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: PinPornProducer]
#21830508 - 06/19/15 10:53 PM (8 years, 7 months ago) |
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Now just forget about them for a while
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: PinPornProducer]
#21830573 - 06/19/15 11:14 PM (8 years, 7 months ago) |
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Well thy are in my room I can see them rite now in the shadows lol so it will be very hard to ignore
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21831506 - 06/20/15 07:26 AM (8 years, 7 months ago) |
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Is it ok to keep the jars in my closet or where should they be. My closet has the normal daylight and it's room temp maybe higher because it's summer. Are they ok or should I put em somewhere else ?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21831542 - 06/20/15 07:47 AM (8 years, 7 months ago) |
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They're great where they are
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: bodhisatta]
#21831564 - 06/20/15 07:55 AM (8 years, 7 months ago) |
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Thank you. I was hoping so. Now I can just look up at them to see how the growth is doing ill be able to see every stage and I won't have to touch/move them around.
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21831620 - 06/20/15 08:14 AM (8 years, 7 months ago) |
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shroomyaxn



Registered: 11/13/11
Posts: 276
Last seen: 2 years, 2 months
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21832490 - 06/20/15 01:58 PM (8 years, 7 months ago) |
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Quote:
Devon420 said:
[/url] 
Dope
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TravelAgency
The ongoing "wow"

Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: shroomyaxn]
#21832504 - 06/20/15 02:00 PM (8 years, 7 months ago) |
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Sick art! She's a keeper!
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: TravelAgency]
#21836114 - 06/21/15 10:05 AM (8 years, 7 months ago) |
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Got the SGFC ready to go. Got alot of time on my hands waiting for my jars to colonize
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21836151 - 06/21/15 10:14 AM (8 years, 7 months ago) |
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Looking good man
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: PinPornProducer]
#21852284 - 06/24/15 08:11 PM (8 years, 7 months ago) |
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Ok guys so I noc'd up last Friday 19th and I have the jars in all random places in my appt to test out the best spot for growth. I'm still seeing nothing. I put alot if spore solution in em I'm sure to much but there's no puddling. I'm just patient I guess. I made a mistake nocking them up I didn't shake the first and last syringe lol but I shook the second one cuz I remembered and forgot again lol. I used almost all the 3 syringes for just 10 jars I have 3 quarter syringes left. Ill see what happens in the next few days tho.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21852333 - 06/24/15 08:20 PM (8 years, 7 months ago) |
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It can take 2-3 weeks to see signs of growth, like I said before, best to just forget about them for a while. Check em in another week. If you don't have any growth in a few weeks then start worrying. Spores can take a while to germinate. 1 syringe can do 10 jars. You could have threw the moisture content off
Edited by PinPornProducer (06/24/15 08:21 PM)
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: PinPornProducer]
#21852389 - 06/24/15 08:29 PM (8 years, 7 months ago) |
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Oh no please don't say that. They are gonna be fine I feel it lol! How long should I wait to make some more jars up I was thinking next week
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Devon420
ShrOoMeRiTe!!



Registered: 06/05/15
Posts: 2,748
Loc: Canada
Last seen: 8 years, 1 month
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Re: CultVirgin 1st Timer,Do I Have everything guys? [Re: Devon420]
#21852391 - 06/24/15 08:29 PM (8 years, 7 months ago) |
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Or should I try a different method hmmm
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