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Invisiblemicro
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Peptone water
    #2180923 - 12/15/03 10:58 PM (13 years, 5 months ago)

Hey -- I just found 10 500ml bottles of sterile peptone water (.1% with polysorbate 80) with septum plugs being given away for free, so I took them. The bottles (and septum plugs which are built into the lids) are reusable, but I was wondering if I could just use the sterile peptone water instead of karo, etc. .1% sounds like way too little, though....

Also -- the karo tek can be used for shiitakes too, right? I can't search so I thought I'd ask, even though it seems pretty obvious they should be the same....

Thanks!

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Micro


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OfflineRandolph_Carter
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Re: Peptone water [Re: micro]
    #2180932 - 12/15/03 11:00 PM (13 years, 5 months ago)

The bottles will be useful, if you can reuse them...nothing's going to grow on .1% sugar concentration tho....


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Invisiblemicro
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Re: Peptone water [Re: Randolph_Carter]
    #2180954 - 12/15/03 11:09 PM (13 years, 5 months ago)

Yeah -- that's kind of what I figured.  Peptone is mostly protein, but I guess at a .1% concentration it's probably just a wash medium.  Thanks for the response :wink:

They are reusable and autoclavable, so I guess I'll just make PDY broth and sterilize it in them .

Thanks!

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OfflineGranola
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Re: Peptone water [Re: micro]
    #2181207 - 12/16/03 12:28 AM (13 years, 5 months ago)

try it with one anyway


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Invisiblemicro
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Re: Peptone water [Re: Granola]
    #2181270 - 12/16/03 12:51 AM (13 years, 5 months ago)

Yeah -- I might, but I really doubt it's enough to really grow anything. I don't even know why I asked this -- I guess I was hoping it might do something just because it would be so convenient, but .1% should be too little to do anything, I would think, like Randolph said.

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OfflineRandolph_Carter
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Re: Peptone water [Re: micro]
    #2181284 - 12/16/03 12:54 AM (13 years, 5 months ago)

Ya might get some ethiopian-esque bacteria...
but the preservative would probably stop that right quick.
Ya can give me one tho, ifin ya feel generous...jars are a pain for liquid culture.


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"..all those molecules thrashing their kinky little tails, hot for destiny and the street."  Gibson


Nuke baby seals for Jesus!

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Invisiblemicro
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Re: Peptone water [Re: Randolph_Carter]
    #2181313 - 12/16/03 01:07 AM (13 years, 5 months ago)

I PMed a supplier about this.... They would really help, but everything I've seen from Fisher has media already in them. They actually sell PDY broth in these, already made, but it's like $171 for ten of them....

I'm sure someone has to sell these without media, but I have yet to find them....

Difco ref # is 290652 if you're interested -- you should be able to search sites for this #. Fishersci.com sells it.

EDIT: the <=100 ml bottles are like a K bottle -- it's only a septum plug with an aluminum wrapping. You can't use these because if you take the metal wrapping off there's only a septum plug which would just pop off if you tried to sterilize them. The 300mL or more bottles with a screw cap should be autoclavable, though -- the septum plug is built into the screw cap.

--
Micro


Edited by micro (12/16/03 01:14 AM)


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Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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