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OfflineShamanic Scientist
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My first monotub - close fruiting - input welcome *DELETED*
    #21805453 - 06/14/15 09:25 AM (8 years, 7 months ago)

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Offlinerudeboi
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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21805475 - 06/14/15 09:32 AM (8 years, 7 months ago)

:greatjob:

looks really good. When the top is fully colonized give it a day or two and then introduce fruiting conditions.

I wouldn't give it to much FAE, the sub will dry out quicker and the RH will be hard to maintain.


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: rudeboi]
    #21805485 - 06/14/15 09:35 AM (8 years, 7 months ago)

What do you mean by too much FAE? In theory, my windows will allow for close to zero water vapor to escape. Maybe I should leave one of the large windows covered?


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21805495 - 06/14/15 09:37 AM (8 years, 7 months ago)

We don't know because we don't build tubs with Windows. If it works for you great, write up a tek but we can't really help you with an untested design.


--------------------
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Re: My first monotub - close fruiting - input welcome [Re: maddchef]
    #21806103 - 06/14/15 12:16 PM (8 years, 7 months ago)

:whathesaid: Why did you make such big "windows"? Filter holes usually only need to be an inch or two wide at most.


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InvisibleLocN9ne
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Re: My first monotub - close fruiting - input welcome [Re: Myconin]
    #21806469 - 06/14/15 01:06 PM (8 years, 7 months ago)

If you used a casing layer then that bad boy is well beyond ready to fruit.


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: LocN9ne]
    #21807237 - 06/14/15 05:09 PM (8 years, 7 months ago)

Hey LocN9ne,

Why do you say so? I am very eager to move to fruiting, but most of what I read says to what until 100% colonization (which I assume means clear mycelial growth over entire top surface) plus a couple days. Would fruiting now vs. waiting a couple more days likely change my total yield, or how evenly pins appear?


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: Myconin]
    #21807247 - 06/14/15 05:11 PM (8 years, 7 months ago)

Myconin,

I did things this way for two reasons. First, I have this fabric readily available to me for cheap. Since it's both hydrophobic and breathable, I figured it would be great. Second, having large windows makes my monotub modular in the sense that I can cover it up full or partially from the outside; if I want FAE to occur with the outside over a smaller surface area, I can simply cover up a portion of one or both windows.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21807428 - 06/14/15 06:01 PM (8 years, 7 months ago)

In my opinion, best time to introduce fruiting conditions, is once you see the mycelium poking through the top surface of the casing layer.  If you have more casing, you can patch up the mycelium spots right before introducing light and FAE to ensure even pinset.  You should do it immediately.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21808897 - 06/15/15 12:22 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
Myconin,

I did things this way for two reasons. First, I have this fabric readily available to me for cheap. Since it's both hydrophobic and breathable, I figured it would be great. Second, having large windows makes my monotub modular in the sense that I can cover it up full or partially from the outside; if I want FAE to occur with the outside over a smaller surface area, I can simply cover up a portion of one or both windows.




Makes sense to me :thumbup: I was just curious.

Happy Growing :trippinbawelz:


--------------------
"No great mind has ever existed without a touch of madness" - Aristotle
"I have just three things to teach: Simplicity, Patience, Compassion. These three are your greatest treasures." - Lao Tzu
"You've just gotta keep on keepin' on, man. You can't have 'no' in your heart" - Joe Dirt

ThirtyCigarettes said: "All I know is every other thread I see in the Cultivation forum goes like this: QUESTION > ANSWER > DIFFERENT ANSWER > ARGUE > TC COMES AND CLEARS IT UP"


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: misiu007]
    #21810140 - 06/15/15 10:37 AM (8 years, 7 months ago)

I was not able to add any more casing, so I moved to fruiting conditions last night. The monotub is at ~25 degrees celsius, I have an air filter in the closet on low all day, and a 6000K LED on a 12/12 hour light/dark cycle. I can add a couple pictures of my set up within 2-3 days for people to critique.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21810159 - 06/15/15 10:43 AM (8 years, 7 months ago)

your going to be fine bro. just let it do its thing. don't want to keep messing with it just gives the bacteria in the air to get a chance at that beautiful mycelium. looks like your on the right track. cant wait to see pics of this bad boy once fruited


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: Shroom Kitty]
    #21810194 - 06/15/15 10:50 AM (8 years, 7 months ago)

Yes, I'm just anxious. I was a little concerned this morning since the humidity in my monotub was 73%, measured on the upper portion inside the tub. I lightly misted with ~1% H2O2 in water. I should just let it sit now. My understanding is that the fruiting process will take a few days, yes?

A couple points of confusion for me looking ahead are:

- What is the actual technique for harvesting mature fruits? Do people generally pull them out of the substrate, or cut them at the base, or what?
- What is the process for getting multiple flushes? Do you just let it sit under fruiting conditions after each harvest? Is it necessary to mix up the substrate at all after first harvest?


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21810221 - 06/15/15 10:58 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
Myconin,

I did things this way for two reasons. First, I have this fabric readily available to me for cheap. Since it's both hydrophobic and breathable, I figured it would be great. Second, having large windows makes my monotub modular in the sense that I can cover it up full or partially from the outside; if I want FAE to occur with the outside over a smaller surface area, I can simply cover up a portion of one or both windows.




When providing FAE you want the whole sub to get FAE, not just part of it.

Like others have stated, it's hard to give advice on an untested, unknown design. :shrug:


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Re: My first monotub - close fruiting - input welcome [Re: FreeWorldOrder]
    #21810565 - 06/15/15 12:23 PM (8 years, 7 months ago)

It seems that many growers like to cut the shroomies at the sub level with a knife or scissors.  For me, I still lightly twist a shroomie and gently pull, which tends to do very little to no damage to the mycelium surface.  Clusters are little harder to harvest, I cut those as low as I can.

As far as flushed go, I wait until most shroomies are ready to harvest, so if one or two grow prematurely, I pick those off, fan dry them and wait for the flush to grow.  At that point, I harvest all mushrooms, and do a decent spray down of the substrate, keeping it all in fruiting conditions.  This allows me to run the dehydrator for a couple of days, and then shut it off until next harvest.  You don't want to mix sub after each flush.


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: misiu007]
    #21810608 - 06/15/15 12:31 PM (8 years, 7 months ago)

Great, thanks misiu! In terms of misting, is this normally done with pure water, or is it advisable to use a dilute H2O2 solution for misting mycelium? My understanding is that at my current stage contamination is pretty unlikely, but it's something I'm always paranoid about.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21810644 - 06/15/15 12:37 PM (8 years, 7 months ago)

you said you used a casing but im not sure if your using the word right. did you mix coir and verm with your spawn then wait until it was 100% colonized then add a casing layer on top and what we see now has grown through?

or is what you showed in the picture the extent of how colonized it is? if so I would give it a little more time before giving it fruiting conditions

and just mist with regular tap water you don't have to use anything special for that part


Edited by Mdahmer (06/15/15 12:38 PM)


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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome [Re: Mdahmer]
    #21810670 - 06/15/15 12:46 PM (8 years, 7 months ago)

I mixed my spawn with coir/verm and then added a thin layer of coir/verm on top of that when I started. The picture is where that was at after one week.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21810716 - 06/15/15 12:57 PM (8 years, 7 months ago)

oh alright. some people call that a pseudo casing I think or some such but actually casing is when you wait for it to fully colonize then add a layer and let that layer colonize to about 30% or so and fruit. your adding a layer on top to help provide a close, humid micro climate for pins to form.

but when you add the layer right after mixing your spawn I don't know that it really does the same thing it just kinda gets colonized with everything else. I would wait until the whole thing is colonized solid before fruiting.


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Re: My first monotub - close fruiting - input welcome [Re: Mdahmer]
    #21811336 - 06/15/15 04:12 PM (8 years, 7 months ago)

:bigyesnod:


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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: FreeWorldOrder]
    #21813603 - 06/16/15 07:24 AM (8 years, 7 months ago)

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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: Shamanic Scientist]
    #21820005 - 06/17/15 05:58 PM (8 years, 7 months ago)

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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: Shamanic Scientist]
    #21826937 - 06/19/15 07:04 AM (8 years, 7 months ago)

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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21830418 - 06/19/15 10:29 PM (8 years, 7 months ago)

Yes looks like knots.  The discoloring is probably due to metabolites, perfectly normal in fact a sign of health :thumbup:


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Re: My first monotub - close fruiting - input welcome [Re: ShroominMe]
    #21830947 - 06/20/15 12:58 AM (8 years, 7 months ago)

lookin good to me but i havent done a grow in a couple of years soooo... lol
but why didnt you allow it to fully colonize before fruiting? or did u case and it colognize the casing layer?


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21831110 - 06/20/15 02:18 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
Here's what the tub looks like this morning. Some of the small uncolonized patches look like they're starting to fill in will small white "dots." Could these be knots? I'm also wondering if what to make of small areas of discoloration. It's subtle, but you may notice small areas of mycelia that do not appear to be as brilliant-white.





the discoloration is most likely metabolites from the mycelium. You should be concerned you start noticing extremely rapidly growing patches of brighter mycelium. Usually, that's how trich starts!


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Re: My first monotub - close fruiting - input welcome [Re: Drunk3n Duck]
    #21831686 - 06/20/15 08:40 AM (8 years, 7 months ago)

I'm a novice and you might be surprised at how difficult it is to get clear and consistent information going into this stuff for the first time. Many people people seem to think it's optimal to add a casing layer at 100% colonization, and fruit at ~30% of the casing layer being colonized. On the other hand, I've read multiple sources say that a casing layer doesn't matter much for cubensis...

I will post higher quality images once I start seeing pins.

Thanks!


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21831745 - 06/20/15 09:09 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
I'm a novice and you might be surprised at how difficult it is to get clear and consistent information going into this stuff for the first time. Many people people seem to think it's optimal to add a casing layer at 100% colonization, and fruit at ~30% of the casing layer being colonized. On the other hand, I've read multiple sources say that a casing layer doesn't matter much for cubensis...

I will post higher quality images once I start seeing pins.

Thanks!



Casing layers don't really matter much for cubes, but when u do use one, you apply it gets applied at 100%, and fruited at about 30%.


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Re: My first monotub - close fruiting - input welcome [Re: PussyFart]
    #21832042 - 06/20/15 11:16 AM (8 years, 7 months ago)

Should I be concerned that I have some patches that have not been fully colonized yet? I've seen plenty of grow logs with good growth both with and without completely full, solid colonization across the entire substrate.


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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: Shamanic Scientist]
    #21833239 - 06/20/15 05:49 PM (8 years, 7 months ago)

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OfflineShamanic Scientist
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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: Shamanic Scientist]
    #21836043 - 06/21/15 09:48 AM (8 years, 7 months ago)

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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21836101 - 06/21/15 10:02 AM (8 years, 7 months ago)

Yes that means conditions could be better


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Re: My first monotub - close fruiting - input welcome [Re: maddchef]
    #21836112 - 06/21/15 10:04 AM (8 years, 7 months ago)

... and?


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21836504 - 06/21/15 11:14 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
- How should I interpret the fact that most pins, at this these early ones, are sprouting up on the sides of the tub?

- Can someone comment on the morphology of my pins at this stage? Is there anything that might tell me some parameters of my tub to tweak?

Thanks!






Pinning near the sides is due to humidity being the higher in those locations. 



My gut feeling about the pic above is mold.  It just doesnt look healthy.  See how no pins are forming only around those patches of "growth". 
I could be wrong, time will tell. 

You really need to consider going with the 1-2" holes stuffed with poly fill next time around for your mono-tub setup.  4-6 holes drilled near the surface of the casing and 2 at the top opposite from each other.


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Re: My first monotub - close fruiting - input welcome [Re: shroomyaxn]
    #21836555 - 06/21/15 11:26 AM (8 years, 7 months ago)

"See how no pins are forming only around those patches of 'growth'."

I do not understand this sentence.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21836583 - 06/21/15 11:34 AM (8 years, 7 months ago)

neither do I :shrug:

the reason pins form along the sides of the tub is because conditions are best there.
give your sub a nice mist.

what kind of filters do you have in your tub holes? and how large are your holes?


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Re: My first monotub - close fruiting - input welcome [Re: spacechildo]
    #21836599 - 06/21/15 11:40 AM (8 years, 7 months ago)

true, judging by the bluing of the substrate, I'd say they're large and it's pretty dry in there.


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Re: My first monotub - close fruiting - input welcome [Re: natedawgnow]
    #21836643 - 06/21/15 11:52 AM (8 years, 7 months ago)

I think you're probably right. I am using a hydrophobic, breathable fabric that is readily available to me for cheap. It is designed to prevent animal dander, allergens, etc. from getting through. I have been misting at least twice a day, which is basically all I can do during the week. I can cover up some of my windows partially.

Thanks!


Edited by Shamanic Scientist (06/21/15 11:52 AM)


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Re: My first monotub - close fruiting - input welcome [Re: spacechildo]
    #21836653 - 06/21/15 11:56 AM (8 years, 7 months ago)

Take a look at the first couple of posts on this page of the thread. You can see a view from the top on the outside, and from the inside. My window on top is very large, since this is also where my light comes into the tub. From the inside you can see the three other windows I have. Compared to a standard monotub, 3/4 of my windows are large, but I can partially cover them up from the outside.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21836672 - 06/21/15 12:01 PM (8 years, 7 months ago)

Maybe use some tape layered piece over piece to try to effectively make the holes in your tub smaller, then stuff them with poly-fil or something to retain some moisture in there.

since you can't mist often, it might help.


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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21836675 - 06/21/15 12:03 PM (8 years, 7 months ago)

I've seen the pics but I'm still confused. one short side looks like it doesnt have any holes,
same with 1 of the long sides while the other long side seems like it has a 5" hole cut in it.

did you follow any specific tek?


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Re: My first monotub - close fruiting - input welcome [Re: natedawgnow]
    #21840995 - 06/22/15 09:10 AM (8 years, 7 months ago)

Quote:

natedawgnow said:
true, judging by the bluing of the substrate, I'd say they're large and it's pretty dry in there.



I was trying to convey exactly this.^^^

Forget what i said about the "patches of growth".  Those sections that are not colonized could be due to the fact they are too dry.


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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: shroomyaxn]
    #21843327 - 06/22/15 08:38 PM (8 years, 7 months ago)

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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21843548 - 06/22/15 09:25 PM (8 years, 7 months ago)

Now is a good time if you have agar available,  take one from the cluster top left and the one top right just off center from that cluster.  Make 2 transfers to clean it up then agar to grain make a plate for future use, grow each one out to see if they have the potential that your looking for if so make a master slant if not try again


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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: mush madness]
    #21847858 - 06/23/15 09:16 PM (8 years, 7 months ago)

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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21847870 - 06/23/15 09:19 PM (8 years, 7 months ago)

I am curious about the fact that my mushrooms tend to cluster around the patches with no mycelium, and are not growing much from the nice, solid white areas of the tub. What does this indicate?


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Re: My first monotub - close fruiting - input welcome [Re: spacechildo]
    #21848127 - 06/23/15 10:16 PM (8 years, 7 months ago)

Quote:

spacechildo said:
I've seen the pics but I'm still confused. one short side looks like it doesnt have any holes,
same with 1 of the long sides while the other long side seems like it has a 5" hole cut in it.





The fact that there are not holes opposite each other in the tub is causing inadequate FAE. 
Since there was not proper FAE during the first few days in fruiting conditions, pinning is now sporadic.


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Re: My first monotub - close fruiting - input welcome [Re: shroomyaxn]
    #21851046 - 06/24/15 03:31 PM (8 years, 7 months ago)

Noted. I will take this into account before my doing my next tub in a couple of weeks.


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Re: My first monotub - close fruiting - input welcome [Re: shroomyaxn]
    #21851803 - 06/24/15 06:15 PM (8 years, 7 months ago)

Quote:

shroomyaxn said:

Since there was not proper FAE during the first few days in fruiting conditions, pinning is now sporadic.



Pinning is always sporatic with MS......


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Re: My first monotub - close fruiting - input welcome [Re: PussyFart]
    #21852530 - 06/24/15 08:52 PM (8 years, 7 months ago)

This makes sense to me given what I am now seeing. I have pretty nice clusters sprouting up all over now that I've been misting more aggressively. In addition, I have so far had better growth on the side of the tub furtherest from the windows I have on the sides, which is inconsistent with the idea that my sporadic fruiting is due to inadequate FAE. I suspect my main issue on this first monotub, beyond having mycelia derived from MS, is that I have tended to have too much FAE, which caused the more central regions to have a lower humidity level on average.

Anyways, I have started to harvest my first fruits. I have been surprised by how much individuals weigh after drying in a dehydrator overnight. Even though this first tub isn't the most impressive grow in the universe, it's more than enough to satisfy my needs. Thanks to what I've learned from reading and getting feedback here, I am confident that my next tub will be even better despite being derived from MS. I hope to start cloning in the near future.


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Re: My first monotub - close fruiting - input welcome *DELETED* [Re: Shamanic Scientist]
    #21852550 - 06/24/15 08:56 PM (8 years, 7 months ago)

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Re: My first monotub - close fruiting - input welcome [Re: Shamanic Scientist]
    #21854107 - 06/25/15 08:25 AM (8 years, 7 months ago)

Quote:

Shamanic Scientist said:
Can someone tell me why the fruit below has an odd cap phenotype? Note the "hole" in the middle. The mushroom in the first pic can be seen growing (the day before) on the right side of the second pic.








Bacterial blotch?


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