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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Where to transfer from (cube and atl7 on agar)
#21804108 - 06/13/15 10:49 PM (8 years, 7 months ago) |
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I transfered tissue from a b+ fruit body a while ago and all 10 transfers came out clean so far but I can't find sectoring in any and all plates are showing complete tomentose mycellium. Where should I grab a sample?


Also I'm new to atl7 mycelium. These plates are one transfer after spores were put on a plate. Anything stand out that I should transfer?
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CH HELL
Brain Sturgeon


Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Where to transfer from (cube and atl7 on agar) [Re: RyeJar]
#21804143 - 06/13/15 11:00 PM (8 years, 7 months ago) |
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In the first picture I would transfer the two sections at 3 and 8 o clock.
On the ATL I would do 12 and a piece from the strong but slower growing 8 o clock.
CH
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Where to transfer from (cube and atl7 on agar) [Re: CH HELL]
#21804157 - 06/13/15 11:06 PM (8 years, 7 months ago) |
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Your first pic looks just like something I posted today that I was told was fukked so ionno.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Where to transfer from (cube and atl7 on agar) [Re: maddchef]
#21804171 - 06/13/15 11:11 PM (8 years, 7 months ago) |
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Quote:
maddchef said: Your first pic looks just like something I posted today that I was told was fukked so ionno.

i'm not sure what atl7 myc is supposed to look like but that b+ plate looks really bad. definitively not clean.
-------------------- (Yes, the egg is real)
How to post pics
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CH HELL
Brain Sturgeon


Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Where to transfer from (cube and atl7 on agar) [Re: FriedEgg]
#21804191 - 06/13/15 11:17 PM (8 years, 7 months ago) |
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It should be fine just don't touch anything from 9-2 o clock and transfer small pieces.
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Where to transfer from (cube and atl7 on agar) [Re: CH HELL]
#21806240 - 06/14/15 12:44 PM (8 years, 7 months ago) |
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Thanks for the responses. so the 2 plates shown were rendered useless because of old parafilm I used didn't hold up. Picked the plate up and the lid came off so I took the cover off to take photos.
What about those positions make those areas the best to transfer so I can infer which areas to take from my other plates? Any particular reason these plates turned out like they did?
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Where to transfer from (cube and atl7 on agar) [Re: RyeJar]
#21810775 - 06/15/15 01:19 PM (8 years, 7 months ago) |
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24 hour bump. I'll lick agar plates if it scores me some help
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funkyfish77
out



Registered: 06/10/10
Posts: 429
Loc:
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Re: Where to transfer from (cube and atl7 on agar) [Re: RyeJar]
#21811035 - 06/15/15 02:48 PM (8 years, 7 months ago) |
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12 and 3 on first plate . I go for fast white growth. 2nd plate from 12 to 3 look good maybe take some from 5 o'clock to . Good luck.
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thoraxx
Wizard


Registered: 12/27/13
Posts: 580
Loc: Bavaria
Last seen: 4 years, 10 months
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Re: Where to transfer from (cube and atl7 on agar) [Re: funkyfish77]
#21811096 - 06/15/15 03:07 PM (8 years, 7 months ago) |
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When theres no clear sectoring and especially on the initial agar transfer, i just pick a few spots at random Then on your second plates the sectors should be pretty obvious
I dont think its nessecary to dump plates just because the parafilm came loose tho
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CH HELL
Brain Sturgeon


Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Where to transfer from (cube and atl7 on agar) [Re: thoraxx]
#21811199 - 06/15/15 03:34 PM (8 years, 7 months ago) |
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Quote:
thoraxx said: When theres no clear sectoring and especially on the initial agar transfer, i just pick a few spots at random Then on your second plates the sectors should be pretty obvious
I dont think its nessecary to dump plates just because the parafilm came loose tho

Just pick the best looking growth. After tranfering a couple times you won't get much sectoring. It just take doing it a few times to able to spot what you think is best, no one can teach it. They both look good to me with the exception of the fuzz on the first picture at the top, it could be mold or it could just be weak. CH
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Where to transfer from (cube and atl7 on agar) [Re: thoraxx]
#21811470 - 06/15/15 04:49 PM (8 years, 7 months ago) |
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Quote:
thoraxx said: When theres no clear sectoring and especially on the initial agar transfer, i just pick a few spots at random Then on your second plates the sectors should be pretty obvious
I dont think its nessecary to dump plates just because the parafilm came loose tho
It didn't just come loose, the cover (top half of plate) Came off the base and exposed the plate to open air.
Alright I'll get those done today or tomorrow. thanks for the help everyone
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