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Offlinehelncharcar
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Registered: 06/13/15
Posts: 3
Last seen: 8 years, 6 months
Trich or Mycelium - 50/50 casing
    #21803232 - 06/13/15 05:50 PM (8 years, 7 months ago)

I'm curious to know if this is Mycelium or Trich.

I've recently had a mono fall victim to a Trich contamination, so I wanted to check in and see what any of you think about this.

It does not smell bad, but the other two trays I've made up at the same time have fully colonized and look a little different than this one.

I've also got a monotub that looks exactly like this, very spotty (only checked it because two weeks had passed).

All are incubating at 80 degrees in a Tub in Tub Incubator.




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InvisibleMad Season
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Registered: 09/16/12
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Re: Trich or Mycelium - 50/50 casing [Re: helncharcar]
    #21803275 - 06/13/15 06:02 PM (8 years, 7 months ago)

Mycelium. Drop the Temps to room temperature. Incubators give it a better climate for those contaminations to thrive. 65-75 is fine.


--------------------
contam and car window art
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Improve your sterile techniques! (A comprehensive guide to agar)
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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21804080 - 06/13/15 10:35 PM (8 years, 7 months ago)

:werd:

it looks like you just spawned it a few days ago.  that looks pretty normal for just spawning.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21810940 - 06/15/15 02:18 PM (8 years, 7 months ago)

Quote:

Mad Season said:
Mycelium. Drop the Temps to room temperature. Incubators give it a better climate for those contaminations to thrive. 65-75 is fine.




70-75 is optimal in my experience. 65 is slow growth for me.


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InvisibleMad Season
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Re: Trich or Mycelium - 50/50 casing [Re: TheEaglesGift]
    #21810951 - 06/15/15 02:21 PM (8 years, 7 months ago)

It is slow but it also slows contams really well too. I agree 70-75 is best for me too but there's a few TCs who like anywhere in the 60s. Apparently really slows down bacteria.

Also it's nice to see you eagle :smile: you shall see me posting in your aa+ thread soon :wink:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/15/15 02:22 PM)


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21810958 - 06/15/15 02:26 PM (8 years, 7 months ago)

Quote:

Mad Season said:
It is slow but it also slows contams really well too. I agree 70-75 is best for me too but there's a few TCs who like anywhere in the 60s. Apparently really slows down bacteria.

Also it's nice to see you eagle :smile: you shall see me posting in your aa+ thread soon :wink:




I've been incognito for a few weeks. Been busy with myco work, and busy with getting tattooed out of sate. I've been lingering cult/contam boards again though, I can't keep away.

Happy the AA+ is going well for you! Want a PE swab?

Quote:

It is slow but it also slows contams really well too. I agree 70-75 is best for me too but there's a few TCs who like anywhere in the 60s. Apparently really slows down bacteria.




60s slow contam growth, true, but they also slow myc growth. I'd rather get the grains/sub colonized as fast as possibly in the mid 70s, because a colonized grain/sub is more contam resistant than grain/sub at a cooler temp. I'm not trying to split hairs over it, 60s works fine if your spawn is clean and your sub is prepped correctly, anyways.

:thumbup:


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InvisibleMad Season
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Re: Trich or Mycelium - 50/50 casing [Re: TheEaglesGift]
    #21810983 - 06/15/15 02:36 PM (8 years, 7 months ago)

Haha no no it's a fair, valid point. Ideally you want a jar colonized so quickly that contaminations/bacteria never stood a chance, this we agree on. In fact I too prefer 70-75. Just saying what I've been told by a few TCs once or twice.

I would love a PE man! I can't make any promises but if I get my APE to germ and get clean and grow up, I'll send you an APE swab for all the shit you've done. Very generous brother


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21810990 - 06/15/15 02:37 PM (8 years, 7 months ago)

Alright, shooting you a pm. :thumbup:

Sorry op for hijacking your thread.


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Offlinehelncharcar
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Registered: 06/13/15
Posts: 3
Last seen: 8 years, 6 months
Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21817210 - 06/16/15 11:33 PM (8 years, 7 months ago)

SO my suspicions were right, this is the casing today (several days after your comment).



Wondering what the hell could be going on, first a mono, then two trays, and now this?

I've been growing successfully for months, but the only difference between my recent failures and my success is that I spawned the successful casings with jars I had inoculated as opposed to grain transferred. 

/u/MikeBearPig mentioned that it could be the grain transfers, and that Trich is hiding inside the jars and coming out to flourish after I spawn.

Here is a link to my mono failure thread for anyone interested (made on another account before I settled with this one)

http://www.shroomery.org/forums/showflat.php/Number/21791276/page/1

I'd love some insight. not sure really where to go from here.

@Mad Season - you suggested going back to inoculating as possibly building a glove box? I'm keen to go down this road an invest a little more if I can be sure the Trich is infiltrating pre-spawn.

Seems like the previous success proves this, but I've learned its not good to assume anything in this hobby.

Thanks for all the help so far, its all a learning experience.

Here is some pictures of my jars (some totally stalled) from grain transfers around the same time.

I'm trying to decide if I should toss them.



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InvisibleMad Season
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Re: Trich or Mycelium - 50/50 casing [Re: helncharcar]
    #21817267 - 06/16/15 11:50 PM (8 years, 7 months ago)

Well ditch the incubator. Summertime has lots of trich because of the high temps. Pretty much every jar there has bacteria. Here's something I've learned.

Quote:

Mad Season said:
Well there's a difference between colonized and cleanly colonized.

This is colonized:

Lots of members have noticed cobweb and trich from jars that look like this or better. Wet spots are bacteria. Not wet grains or wet spots. To colonize cleanly is the hardest. I had to change my pc times to 2-3 hours to reduce it.

This is (ALMOST) cleanly colonized, that's how hard it is to have it completely clean. This isn't even completely clean:


This is completely clean spawn:



Anyone who's done this for awhile will testify this is the hardest part of the hobby and is what will dictate the success or failure of bulk.



Agar work in a still air box (not glove box) will show trich/bacteria with mycelium and you can transfer a clean wedge to a new dish. You can tiger drop a clean wedge into a jar with a filtered gas exchange hole, you'll have clean spawn like that. Also I recommend upping your pc times by 30 minutes to an hour to kill those pesky bacterial endospores.

I wouldn't toss the colonized wet ones, but I'm telling you how I overcome contaminations.

The wet ones in that quote still made a couple ounces or more in a tray. I just have better success all around when it's clean.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/17/15 12:07 AM)


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Offlinehelncharcar
Stranger
Registered: 06/13/15
Posts: 3
Last seen: 8 years, 6 months
Re: Trich or Mycelium - 50/50 casing [Re: Mad Season]
    #21817373 - 06/17/15 12:42 AM (8 years, 7 months ago)

Very interesting.

Thanks once again for such a thought out reply, its very kind of you.

sounds like Agar might be something to look into, along with ditching the incubator, grain transfers, and upping my PC time.

I live in a building that was built in the 1940s, and restoration wise has not seen much love since (extremely hard to clean, small space)

I'm not going to give up, but I think I will scale down until I find a method that works for me. Its almost certain that I just "got lucky" starting out.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Trich or Mycelium - 50/50 casing [Re: helncharcar]
    #21817434 - 06/17/15 01:02 AM (8 years, 7 months ago)

Learn agar, it's as easy as making jello shots.

Pasty made a killer tek. Pretty hard to fuck up.


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