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Offlinesauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
Bacteria sterilization without harming spores
    #21801338 - 06/13/15 09:30 AM (8 years, 7 months ago)

What kill bacteria but not cubensis spores? Need to make sure spore print is without contamination. Drying works?


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OfflinePurpleSticky
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Registered: 05/11/15
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Last seen: 8 years, 5 months
Re: Bacteria sterilization without harming spores [Re: sauroman1]
    #21801349 - 06/13/15 09:32 AM (8 years, 7 months ago)

Drying probably won't work. I think the best bet would be to go to agar and try to get a clean sample there.

Why do you think the print is contaminated?


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Invisiblemushmagic
supporting radical habits
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Registered: 03/21/12
Posts: 5,372
Loc: Candyland
Re: Bacteria sterilization without harming spores [Re: PurpleSticky]
    #21801397 - 06/13/15 09:46 AM (8 years, 7 months ago)

Virtually no print is contaminant free. This is what we have agar for.

Also remember you don't need very many spores scraped off of a print. This will also help in the prevention of scraping up a bunch of contaminants along with your spores considering you're working with such a small portion of the print.


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Offlinesauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
Re: Bacteria sterilization without harming spores [Re: mushmagic]
    #21813044 - 06/15/15 11:51 PM (8 years, 7 months ago)

Might peroxide sterilization work?


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"You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna


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Offlinesaintburger
Helper


Registered: 09/18/14
Posts: 469
Last seen: 1 month, 18 days
Re: Bacteria sterilization without harming spores [Re: sauroman1]
    #21813081 - 06/16/15 12:04 AM (8 years, 7 months ago)

You put the spores on agar with an inoculation loop and chase the clean, healthy mycelium through transferring it away from any contaminants.


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Set goals for yourself, or wander around aimlessly.

"One day Alice came to a fork in the road
and saw a Cheshsire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'"


Edited by saintburger (06/16/15 12:05 AM)


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: Bacteria sterilization without harming spores [Re: saintburger]
    #21813109 - 06/16/15 12:16 AM (8 years, 7 months ago)

Antibiotic agar :thumbup:


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As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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