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sauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
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Bacteria sterilization without harming spores
#21801338 - 06/13/15 09:30 AM (8 years, 7 months ago) |
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What kill bacteria but not cubensis spores? Need to make sure spore print is without contamination. Drying works?
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna
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PurpleSticky
Stranger
Registered: 05/11/15
Posts: 21
Last seen: 8 years, 5 months
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Re: Bacteria sterilization without harming spores [Re: sauroman1]
#21801349 - 06/13/15 09:32 AM (8 years, 7 months ago) |
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Drying probably won't work. I think the best bet would be to go to agar and try to get a clean sample there.
Why do you think the print is contaminated?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Bacteria sterilization without harming spores [Re: PurpleSticky]
#21801397 - 06/13/15 09:46 AM (8 years, 7 months ago) |
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Virtually no print is contaminant free. This is what we have agar for.
Also remember you don't need very many spores scraped off of a print. This will also help in the prevention of scraping up a bunch of contaminants along with your spores considering you're working with such a small portion of the print.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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sauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
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Re: Bacteria sterilization without harming spores [Re: mushmagic]
#21813044 - 06/15/15 11:51 PM (8 years, 7 months ago) |
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Might peroxide sterilization work?
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna
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saintburger
Helper


Registered: 09/18/14
Posts: 469
Last seen: 1 month, 18 days
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Re: Bacteria sterilization without harming spores [Re: sauroman1]
#21813081 - 06/16/15 12:04 AM (8 years, 7 months ago) |
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You put the spores on agar with an inoculation loop and chase the clean, healthy mycelium through transferring it away from any contaminants.
-------------------- Set goals for yourself, or wander around aimlessly. "One day Alice came to a fork in the road and saw a Cheshsire cat in a tree. 'Which road do I take?' she asked. 'Where do you want to go?' was his response 'I don't know', Alice answered. 'Then', said the cat, 'it doesn't matter.'"
Edited by saintburger (06/16/15 12:05 AM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Bacteria sterilization without harming spores [Re: saintburger]
#21813109 - 06/16/15 12:16 AM (8 years, 7 months ago) |
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Antibiotic agar
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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