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Invisibleoontribe
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Registered: 01/14/15
Posts: 3,570
millet jar contam?
    #21797204 - 06/12/15 12:13 PM (8 years, 7 months ago)

Greysh mycelium.



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Invisiblemaddchef
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Re: millet jar contam? [Re: oontribe]
    #21797211 - 06/12/15 12:15 PM (8 years, 7 months ago)

Green not gray. To the trash it goes


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleoontribe
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Re: millet jar contam? [Re: maddchef]
    #21797262 - 06/12/15 12:25 PM (8 years, 7 months ago)

Should i pc it before tossing it?


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Invisibleoontribe
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Re: millet jar contam? [Re: oontribe]
    #21797280 - 06/12/15 12:30 PM (8 years, 7 months ago)

And there is a lot of black things on the other side of the jar!


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: millet jar contam? [Re: oontribe]
    #21797345 - 06/12/15 12:45 PM (8 years, 7 months ago)

Quote:

oontribe said:
Should i pc it before tossing it?



You should.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleoontribe
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Re: millet jar contam? [Re: Buck513]
    #21797358 - 06/12/15 12:48 PM (8 years, 7 months ago)

Ewwwwww the black contam looks disgusting.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: millet jar contam? [Re: oontribe]
    #21797376 - 06/12/15 12:49 PM (8 years, 7 months ago)

Lick it


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleoontribe
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Re: millet jar contam? [Re: Buck513]
    #21797419 - 06/12/15 12:57 PM (8 years, 7 months ago)

Does it taste good?


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OfflineMachiavelliavore
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Re: millet jar contam? [Re: oontribe]
    #21797584 - 06/12/15 01:32 PM (8 years, 7 months ago)

Mycellium can look a bit gray at the start, but that looks like Trich to me.

If I recall from what you said in previous threads:

Noc'd with syringe (then shaken.)

Noc'd with lid-pops in a SAB.

Suggests that either you have a contaminated syringe or a problem with SAB tecnique.  The black stuff looks like it might be part of the grains, but it's hard to see.

Glad you saved your syringe instead of using it up redoing your nocs.  I'd suggest you make some invitro v-tek jars out of dollartree betty crcoker twist tops.
http://www.shroomery.org/forums/showflat.php/Number/19035259#19035259

Inocculate a few of them with a few drops of spore solution on one point, inspect for contams, G2G a quart from a clean petri, then G2G the rest of your quarts.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleoontribe
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Re: millet jar contam? [Re: Machiavelliavore]
    #21797619 - 06/12/15 01:42 PM (8 years, 7 months ago)

Thanks for the info man...i do need to start working with agar.
yea its not myc. it looks like gray dust covering the millets.
the black contam looks like ALOT of tiny black things covering a certain part of the jar.
This is the only jar that has condensation inside it and the only contaminated one the other 13 are doing good...
Right now iam pcing this jar in the kicthen...and thats where iam keeping the other ones inside a transparent rack.
can the spores be transmited throw the pc's steam? Should i have transferred the other jars in another place while pcing this one?


Edited by oontribe (06/12/15 01:48 PM)


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InvisibleBuck513

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Posts: 5,682
Re: millet jar contam? [Re: oontribe]
    #21797645 - 06/12/15 01:47 PM (8 years, 7 months ago)

Quote:

oontribe said:
Thanks for the info man...i do need to start working with agar.
yea its not myc. it looks like gray dust covering the millets.
the black contam looks like ALOT of tiny black things covering a certain part of the jar.
This is the only jar that has condensation inside it and the only contaminated one the other 13 are doing good...
Right now iam pcing this jar in the kicthen...and thats where iam keeping the other ones!
can the spores be transmited throw the pc's steam? Should i have transferred the other jars in another place while pcing this one?



It IS mycelium, just not the kind you want.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleoontribe
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Re: millet jar contam? [Re: Buck513]
    #21797653 - 06/12/15 01:49 PM (8 years, 7 months ago)

ohh yea thats what i meant.


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: millet jar contam? [Re: oontribe]
    #21797668 - 06/12/15 01:55 PM (8 years, 7 months ago)

Quote:

oontribe said:
ohh yea thats what i meant.



:orly:


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Invisibleoontribe
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Re: millet jar contam? [Re: Buck513]
    #21797678 - 06/12/15 01:57 PM (8 years, 7 months ago)

Something else is taking the seeds other then the cubensis myc.


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OfflineMachiavelliavore
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Re: millet jar contam? [Re: oontribe]
    #21798146 - 06/12/15 03:45 PM (8 years, 7 months ago)

Damn near impossible.  I've never even PC'd a jar to get rid of it, I just try to open them downwind of me and mist down any spore matter immediately outside.  Trich spores seem to be everywhere anyway, just avoid opening them inside.  The rest of your jars are already being exposed to trich spores externally, but are sealed, so it doesn't matter.  Glad to hear the rest of your jars are fine.

You can easily make some good clones or the occassional opportunistic isolate with just grain petris.  In your SAB, rip the stipe of one of your best clusters (lots of mushrooms, but not lots of little underdeveloped ones,) open at the base, cut out a couple tiny samples with a flame sterilized knife, and dump them into about 1/8 cup grains.  Do like 2 or 3 if the cluster is realy good.  Some will contam, most won't.  When you get a good one, transfer half to another 1/8th cup, save the rest as a master culture, then g2g that new 1/8th to a quart.

I've done grain to grain tansfers away from contams, taken aggressive looking isolated  sectors, etc.  It would have been easier with agat, but you can do all kinds of shit without it, and you certainly don't need it for cloning.  If you have old PF jars you can convert them to grain petris easily with a postal tyvek disk.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisibleoontribe
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Re: millet jar contam? [Re: Machiavelliavore]
    #21800359 - 06/13/15 12:52 AM (8 years, 7 months ago)

Woooow cool


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OfflineShroominMe
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Re: millet jar contam? [Re: oontribe]
    #21800408 - 06/13/15 01:09 AM (8 years, 7 months ago)

Just open up outside, dispose of the contaminated sub

Put some bleach in your jar and swirl around

Add some water and rinse

Put in some antibacterial soap add water till you get bubbles and swish around a while.

Before you go back inside make sure you 'disinfect' yourself with Lysol

If possible remove all your clothes and spray

Not possible in most urban environments :smile:


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Invisibleoontribe
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Re: millet jar contam? [Re: ShroominMe]
    #21800480 - 06/13/15 01:50 AM (8 years, 7 months ago)

Ohh thanks man...i ve already pcd it and tossed it.


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