Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Peace
    #21796481 - 06/12/15 08:58 AM (8 years, 7 months ago)

Fuck it all to hell. I just saw green in a bag, again. I don't know how to be any more careful, I really don't. I have gone over and over my technique.

The bags are run long enough to kill mold so that's not it. My bags are opened the shortest amount I can get away with and inoculated with what appears to be 100% clean wedges.

I've tested my hood by opening a plate, facing it towards the filter and moving it over every inch so if there was a leak it would be easily detected, the plate stayed clean.

This used to be a fun hobby, used to. Now I'm all fucking stressed out because everything I do I have to throw away.

I'm out.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796515 - 06/12/15 09:06 AM (8 years, 7 months ago)

That's nice dear.

Maybe stop using bags?


Extras: Filter Print Post Top
OfflineSksoul
Pan handler
 User Gallery


Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: Inocuole]
    #21796545 - 06/12/15 09:17 AM (8 years, 7 months ago)

still air box, pp5, whole brown rice, LI/LC, coir/verm.

You seem to have been over everything, but you are clearly missing something. Try smaller jars (pints or even half pints) and noc up a shit ton of them... Keep it simple til you get the results you are looking for.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Sksoul]
    #21796587 - 06/12/15 09:33 AM (8 years, 7 months ago)

I didn't have any better results with jars. It's either something I'm mistaking for mycelium on my plates or I'm just ignorant.

It just happened one day out of the blue and now I can't grow shit.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796595 - 06/12/15 09:35 AM (8 years, 7 months ago)

How much success have you had with bags in the past? I suggest switch to jars for a single grow. That way you at least narrow the vector.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Pastywhyte]
    #21796603 - 06/12/15 09:39 AM (8 years, 7 months ago)

I had very limited success with jars before I switched if you remember.

But I could never nail down the cause. It would kinda come and go but I'm not the best at tracking my cultures. Although I scrapped every culture I was working with and went back to spore->agar.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflineSksoul
Pan handler
 User Gallery


Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: maddchef]
    #21796639 - 06/12/15 09:49 AM (8 years, 7 months ago)

pc issue? Have you left jars or bags sealed after pc'ing to see if they get contaminates growing in them?

If it isn't your grain prep, your sterilization, or your culture then its how you are inoculating. It has to be one of those. That's why I suggested lots of tiny jars. You can test a ton of variables in a short time.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Sksoul]
    #21796699 - 06/12/15 10:00 AM (8 years, 7 months ago)

It's either something I'm missing on my plates or my technique.

I dunno what else to do for my technique. I've worked commercially in a hood and am quite familiar with usp 797 and aseptic techniques.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21796764 - 06/12/15 10:16 AM (8 years, 7 months ago)

I wanna say its not my technique since all of my agar work is clean ie: no bacteria or colonies

But I wanna say its not my cultures because I always use agar and it always looks exceptional.

Fucked if I know


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflineSksoul
Pan handler
 User Gallery


Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: maddchef]
    #21796775 - 06/12/15 10:20 AM (8 years, 7 months ago)

Even if your technique was shit, you'd probably not have as many problems as you do. I g2g using ziplocks in a sab, fer fucks sake and don't have contam issues at all.

I doubt you could have so many crap cultures, though... clean growth over a few transfers is enough. Now I'm stuck. Wanted to help you troubleshoot, but sorry buddy.


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Extras: Filter Print Post Top
InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Peace [Re: maddchef]
    #21796809 - 06/12/15 10:31 AM (8 years, 7 months ago)

yup, switch to jars and see if that helps.

and run through your sterile technique to make sure you arent using any short cuts (such as reusing old gloves)


--------------------

(Yes, the egg is real)

How to post pics


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: FriedEgg]
    #21796858 - 06/12/15 10:46 AM (8 years, 7 months ago)

Been through the same thing with jars


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796868 - 06/12/15 10:48 AM (8 years, 7 months ago)

Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.

If those work it's your grain prep.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21796924 - 06/12/15 11:04 AM (8 years, 7 months ago)

For your viewing pleasure take a look at these plates and tell me what you see


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: Peace [Re: maddchef]
    #21796931 - 06/12/15 11:06 AM (8 years, 7 months ago)

Those are not clean plates man


Extras: Filter Print Post Top
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796933 - 06/12/15 11:06 AM (8 years, 7 months ago)

First one looks good, second has a spot of bacteria at about 8 o'clock.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: Peace [Re: 36fuckin5]
    #21796936 - 06/12/15 11:08 AM (8 years, 7 months ago)

2nd plate looks fkd from 10 - 3
And the first plate doesn't look right to me either but :shrug:


Edited by PinPornProducer (06/12/15 11:09 AM)


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796946 - 06/12/15 11:10 AM (8 years, 7 months ago)

:uhoh:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21796951 - 06/12/15 11:12 AM (8 years, 7 months ago)

The second plate came from the leading edge of the first plate before "it" grew out.

Yeah neither are good. The second plate has a piece I could grab from but this is what is piggybacking all my cultures and I cannot seem to get rid of it.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796956 - 06/12/15 11:13 AM (8 years, 7 months ago)

Tried the agar sandwich yet?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21796961 - 06/12/15 11:14 AM (8 years, 7 months ago)

I also have plates like this one which will one day "fuzz" up.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796963 - 06/12/15 11:15 AM (8 years, 7 months ago)

Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: Peace [Re: maddchef]
    #21796968 - 06/12/15 11:16 AM (8 years, 7 months ago)

That plate looks like vert


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21796971 - 06/12/15 11:17 AM (8 years, 7 months ago)

I don't think the sammich will help with mold will it? I've transferred a million times, started over from spore, etc.

Tomorrow I will clone a fruit but I'm afraid it will ride along somehow. I just need a 100% clean culture so that I can concentrate on other variables.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21796988 - 06/12/15 11:21 AM (8 years, 7 months ago)

Take a transfer from said clone culture from the very leading edge of the culture BEFORE the culture hits the walls of the petri dish.  Taking a transfer no larger than a grain of rice from the leading edge of the culture gives the culture a window of opportunity to out run any contams may they be present.  Window closes once the myce hits the walls of the petris though.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Peace [Re: hamloaf]
    #21796996 - 06/12/15 11:22 AM (8 years, 7 months ago)

Quote:

hamloaf said:
Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!




:whathesaid: Those all have mold meshed in riding along. Going to be really tricky to clean up. Like ham suggested I would start over from spores, use as few as possible.  Good luck.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Pastywhyte]
    #21797002 - 06/12/15 11:24 AM (8 years, 7 months ago)

:frown:

Really depressing as I have already started from spore again and again run into this


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21797013 - 06/12/15 11:27 AM (8 years, 7 months ago)

Would the fruitbody clone not be a better option or would it somehow mingle itself into the fruit itself?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: Peace [Re: maddchef]
    #21797021 - 06/12/15 11:28 AM (8 years, 7 months ago)

Try making an sab of glovebox to do transfers in. Looks like you could salvage some clean growth from the second plate anywhere from 4 - 9 o'clock, just make a couple more transfers to be sure it's clean.
I throw everything I'm working with in this after wiping everything down with bleach water, give a quick spray of Lysol in the box, close it up and wait 10 min then go to work. I cut a little hole in the lower right side of the box for a flame sterilizing port. Just keep the flame a few inches away from the hole and sterilize the scalpel between transfers. This procedure works great for me


Edited by PinPornProducer (06/12/15 11:30 AM)


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21797026 - 06/12/15 11:29 AM (8 years, 7 months ago)

Suck it up, and keep trying, man.  Failure is merely a stepping stone to success.  For some of us that stair well can have many steps.

Fruit body clone is great.  The best way to take a clone, imo, is from young pinhead.  Cloning from young pinhead generates the most vigorous culture due to the rate of expansion the mycelium is going through in a young pinhead.  Young pinheads are doubling in size every 24 hours.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: hamloaf]
    #21797037 - 06/12/15 11:32 AM (8 years, 7 months ago)

Right but is there any chance for mold to piggyback if I take a core sample? I don't think that's possible but wanna make sure


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21797066 - 06/12/15 11:39 AM (8 years, 7 months ago)

There's always a chance of that.  Taking a core sample though virtually eliminates your chances of mold of bacteria spores piggybacking on your culture sample, as long as you are clean.  Just be as aseptic as possible.  You got this, maddchef!  Nothin' to it, but to do it!


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: hamloaf]
    #21797078 - 06/12/15 11:41 AM (8 years, 7 months ago)

I've never seen mold intertwined with myc. I always see them bump up against each other and pretty much stop.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisibleMikeBearPig
Not liked.
 User Gallery
Registered: 07/15/14
Posts: 2,319
Re: Peace [Re: maddchef]
    #21797093 - 06/12/15 11:45 AM (8 years, 7 months ago)

As for a person who does everything as lazy as possible, I can tell you your vector is not your technique.

It is your inoculate..  I am convinced people confuse other aspects of their contamination with something that looks so convincingly clean. 

I agree with them, switch to jars and start fresh with something you have not made.  Maybe get a new syringe and go from there..


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21797096 - 06/12/15 11:45 AM (8 years, 7 months ago)

Frank had some real issues with meshed in mold a few years ago. It was in nearly all his cultures. Its not unheard of.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: MikeBearPig]
    #21797099 - 06/12/15 11:46 AM (8 years, 7 months ago)

On the full plate, will someone explain how to identify or how you know there is intermingled mold?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21797170 - 06/12/15 12:02 PM (8 years, 7 months ago)

Its usually thin and tomentose looking. In the thinnest parts look carefully to see if the myc is organised or random.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Pastywhyte]
    #21797172 - 06/12/15 12:03 PM (8 years, 7 months ago)

I don't see it


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Peace [Re: maddchef]
    #21797226 - 06/12/15 12:18 PM (8 years, 7 months ago)

Quote:

maddchef said:
For your viewing pleasure take a look at these plates and tell me what you see





The first plate its pretty much spread throughout but the second one its mostly concentrated between 10-3 o'clock.  On a plate like that the only place I would transfer from is 6 o clock.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Pastywhyte]
    #21797802 - 06/12/15 02:25 PM (8 years, 7 months ago)

I see it well on those. I was asking about the full plate I posted.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21798460 - 06/12/15 04:47 PM (8 years, 7 months ago)

Well I will make a clone plate tomorrow and transfer it at least once. I'll even make a super duper cool thread where you all can tell me how wonderful my plate looks.

I will then either drop wedges in bags or go li, whichever would be suggested. As I will have bags prepped and sitting around you can pick your poison.

Dunno what else to try really


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: hamloaf]
    #21798771 - 06/12/15 05:49 PM (8 years, 7 months ago)

This

Quote:

36fuckin5 said:
Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.

If those work it's your grain prep.






Or this

Quote:

hamloaf said:
Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!




Anyone doing anything on a large scale will one day run into issues.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21798869 - 06/12/15 06:09 PM (8 years, 7 months ago)

Lol I'm nowhere even near hobby scale much less large. I just do more than I need with the knowledge imma have to throw most of it out.

Azur do you see any issue from cloning, is there ANY way mold could be intermingled in the actual tissue of the fruitbody?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/12/15 07:23 PM)


Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: maddchef]
    #21798884 - 06/12/15 06:11 PM (8 years, 7 months ago)

You have a fruit on a contam'd sub?  I personally wouldn't clone it unless that's all you've got. I'm about to send you a link.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21798906 - 06/12/15 06:15 PM (8 years, 7 months ago)

Nah its outdoors


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: maddchef]
    #21799088 - 06/12/15 06:58 PM (8 years, 7 months ago)

Outdoor fruits are always filthy


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21799135 - 06/12/15 07:11 PM (8 years, 7 months ago)

I consider any fruit to be filthy. It shouldn't affect the viability of the tissue sample though right?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 24 days
Re: Peace [Re: maddchef]
    #21799283 - 06/12/15 07:48 PM (8 years, 7 months ago)

I just wipe the outside of the stipe with rubbing alcohol, tear the stipe in half and cut a piece of tissue from the inside. The lower of the stipe the more aggresive. 99% success rate doing this method


Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: PinPornProducer]
    #21799292 - 06/12/15 07:49 PM (8 years, 7 months ago)

I would do that in a sab and not in front of your hood


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21799357 - 06/12/15 07:58 PM (8 years, 7 months ago)

Agreed or else I'll just blow shit all over my sample.

Damn now I gotta go find a cardboard box and some saran wrap


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
OfflineSksoul
Pan handler
 User Gallery


Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: maddchef]
    #21799506 - 06/12/15 08:40 PM (8 years, 7 months ago)

Quote:

maddchef said:
Agreed or else I'll just blow shit all over my sample.

Damn now I gotta go find a cardboard box and some saran wrap




Sounds like you might have a few monotubs lying around not doing anything. Turn one on its side. :grin:


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Sksoul]
    #21799577 - 06/12/15 09:05 PM (8 years, 7 months ago)

On its side? You suggesting I work through the non existent lid?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Peace [Re: maddchef]
    #21800137 - 06/12/15 11:28 PM (8 years, 7 months ago)

:popcorn:


Extras: Filter Print Post Top
OfflineSksoul
Pan handler
 User Gallery


Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: invitro]
    #21800347 - 06/13/15 12:45 AM (8 years, 7 months ago)

I've used cardboard boxes with success, but they are pretty flimsy after spraying and dusty if you don't spray enough. I guess you could tape foil to the cardboard and then spray the box down.

You can make arm holes in the lid of a monotub and tape em off when done or drape plastic wrap over the open side... Use a box if you want, I mean, things don't seem like they can get any worse. Good luck cleaning up your samples or getting cleaner ones!


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


Extras: Filter Print Post Top
InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: Peace [Re: Sksoul]
    #21800366 - 06/13/15 12:54 AM (8 years, 7 months ago)

If your agar's all fucked up, maybe try shooting some spores on grain...
You do any edibles?
Also check your pasteurization temps! slower and lower = better...


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


Extras: Filter Print Post Top
OfflineShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: Peace [Re: Sksoul]
    #21800381 - 06/13/15 12:59 AM (8 years, 7 months ago)

100% success rate 0 contams baby!
:lolsy:


Extras: Filter Print Post Top
Invisiblemaddchef
Vaginal escape artist
 User Gallery


Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: ShroominMe]
    #21803834 - 06/13/15 09:03 PM (8 years, 7 months ago)

Alright so today I cloned a fruit from a tissue sample, duh. Also made a few transfers to sectioned plates on half strength lme agar.

The thought is to turn the mushroom myc a bit more rhizomorphic and encourage it to grow outwards in search of food. I will likely make at least 2 more transfers but for now we will see how these few plates go.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,767 43 07/08/23 07:04 AM
by Screwup
* Funkyballoon's NEW Tissue Culture Technique??? KitMarlowe 1,863 2 01/08/02 11:43 AM
by Visigoth
* liquid culture pr? socratesmind 2,029 8 01/18/03 06:45 PM
by ISH
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,285 38 05/11/01 11:08 AM
by MAIA
* 1st Liquid Culture, arrrgh! Seril 14,295 10 02/28/06 08:35 PM
by Limelight_Liver
* Corn Starch Liquid Mycelium Culture AlienPrimate 8,364 7 09/09/17 11:22 PM
by ComebackKid
* Liquid Culture to Bulk Subtrate? Fluxburn 2,886 5 05/12/05 03:50 PM
by MushroomFriend
* Psilocybe Cubensis Mycelial Culture Technique jardinero 620 1 03/08/04 04:55 PM
by simplemachine

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,073 topic views. 27 members, 235 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.043 seconds spending 0.012 seconds on 14 queries.