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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Peace
#21796481 - 06/12/15 08:58 AM (8 years, 7 months ago) |
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Fuck it all to hell. I just saw green in a bag, again. I don't know how to be any more careful, I really don't. I have gone over and over my technique.
The bags are run long enough to kill mold so that's not it. My bags are opened the shortest amount I can get away with and inoculated with what appears to be 100% clean wedges.
I've tested my hood by opening a plate, facing it towards the filter and moving it over every inch so if there was a leak it would be easily detected, the plate stayed clean.
This used to be a fun hobby, used to. Now I'm all fucking stressed out because everything I do I have to throw away.
I'm out.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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That's nice dear.
Maybe stop using bags?
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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still air box, pp5, whole brown rice, LI/LC, coir/verm.
You seem to have been over everything, but you are clearly missing something. Try smaller jars (pints or even half pints) and noc up a shit ton of them... Keep it simple til you get the results you are looking for.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: Sksoul]
#21796587 - 06/12/15 09:33 AM (8 years, 7 months ago) |
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I didn't have any better results with jars. It's either something I'm mistaking for mycelium on my plates or I'm just ignorant.
It just happened one day out of the blue and now I can't grow shit.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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How much success have you had with bags in the past? I suggest switch to jars for a single grow. That way you at least narrow the vector.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I had very limited success with jars before I switched if you remember.
But I could never nail down the cause. It would kinda come and go but I'm not the best at tracking my cultures. Although I scrapped every culture I was working with and went back to spore->agar.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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pc issue? Have you left jars or bags sealed after pc'ing to see if they get contaminates growing in them?
If it isn't your grain prep, your sterilization, or your culture then its how you are inoculating. It has to be one of those. That's why I suggested lots of tiny jars. You can test a ton of variables in a short time.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: Sksoul]
#21796699 - 06/12/15 10:00 AM (8 years, 7 months ago) |
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It's either something I'm missing on my plates or my technique.
I dunno what else to do for my technique. I've worked commercially in a hood and am quite familiar with usp 797 and aseptic techniques.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I wanna say its not my technique since all of my agar work is clean ie: no bacteria or colonies
But I wanna say its not my cultures because I always use agar and it always looks exceptional.
Fucked if I know
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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Even if your technique was shit, you'd probably not have as many problems as you do. I g2g using ziplocks in a sab, fer fucks sake and don't have contam issues at all.
I doubt you could have so many crap cultures, though... clean growth over a few transfers is enough. Now I'm stuck. Wanted to help you troubleshoot, but sorry buddy.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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yup, switch to jars and see if that helps.
and run through your sterile technique to make sure you arent using any short cuts (such as reusing old gloves)
-------------------- (Yes, the egg is real)
How to post pics
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Been through the same thing with jars
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.
If those work it's your grain prep.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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For your viewing pleasure take a look at these plates and tell me what you see
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
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Those are not clean plates man
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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First one looks good, second has a spot of bacteria at about 8 o'clock.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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2nd plate looks fkd from 10 - 3 And the first plate doesn't look right to me either but
Edited by PinPornProducer (06/12/15 11:09 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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The second plate came from the leading edge of the first plate before "it" grew out.
Yeah neither are good. The second plate has a piece I could grab from but this is what is piggybacking all my cultures and I cannot seem to get rid of it.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Tried the agar sandwich yet?
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I also have plates like this one which will one day "fuzz" up.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Dirty inoculm. Your stock cultures are dirty man. Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again. Trust me, I know how hard, and un motivating it is when you get a contam break out. Only thing that can be done, though is to clean everything out, and begin again. Sorry, maddchef. Things will get better. How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again. Good luck!
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
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That plate looks like vert
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I don't think the sammich will help with mold will it? I've transferred a million times, started over from spore, etc.
Tomorrow I will clone a fruit but I'm afraid it will ride along somehow. I just need a 100% clean culture so that I can concentrate on other variables.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Take a transfer from said clone culture from the very leading edge of the culture BEFORE the culture hits the walls of the petri dish. Taking a transfer no larger than a grain of rice from the leading edge of the culture gives the culture a window of opportunity to out run any contams may they be present. Window closes once the myce hits the walls of the petris though.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
hamloaf said: Dirty inoculm. Your stock cultures are dirty man. Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again. Trust me, I know how hard, and un motivating it is when you get a contam break out. Only thing that can be done, though is to clean everything out, and begin again. Sorry, maddchef. Things will get better. How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again. Good luck!
Those all have mold meshed in riding along. Going to be really tricky to clean up. Like ham suggested I would start over from spores, use as few as possible. Good luck.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Really depressing as I have already started from spore again and again run into this
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Would the fruitbody clone not be a better option or would it somehow mingle itself into the fruit itself?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
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Try making an sab of glovebox to do transfers in. Looks like you could salvage some clean growth from the second plate anywhere from 4 - 9 o'clock, just make a couple more transfers to be sure it's clean. I throw everything I'm working with in this after wiping everything down with bleach water, give a quick spray of Lysol in the box, close it up and wait 10 min then go to work. I cut a little hole in the lower right side of the box for a flame sterilizing port. Just keep the flame a few inches away from the hole and sterilize the scalpel between transfers. This procedure works great for me
Edited by PinPornProducer (06/12/15 11:30 AM)
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Suck it up, and keep trying, man. Failure is merely a stepping stone to success. For some of us that stair well can have many steps.
Fruit body clone is great. The best way to take a clone, imo, is from young pinhead. Cloning from young pinhead generates the most vigorous culture due to the rate of expansion the mycelium is going through in a young pinhead. Young pinheads are doubling in size every 24 hours.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Right but is there any chance for mold to piggyback if I take a core sample? I don't think that's possible but wanna make sure
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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There's always a chance of that. Taking a core sample though virtually eliminates your chances of mold of bacteria spores piggybacking on your culture sample, as long as you are clean. Just be as aseptic as possible. You got this, maddchef! Nothin' to it, but to do it!
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I've never seen mold intertwined with myc. I always see them bump up against each other and pretty much stop.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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MikeBearPig
Not liked.

Registered: 07/15/14
Posts: 2,319
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As for a person who does everything as lazy as possible, I can tell you your vector is not your technique.
It is your inoculate.. I am convinced people confuse other aspects of their contamination with something that looks so convincingly clean.
I agree with them, switch to jars and start fresh with something you have not made. Maybe get a new syringe and go from there..
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Frank had some real issues with meshed in mold a few years ago. It was in nearly all his cultures. Its not unheard of.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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On the full plate, will someone explain how to identify or how you know there is intermingled mold?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Its usually thin and tomentose looking. In the thinnest parts look carefully to see if the myc is organised or random.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I don't see it
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
maddchef said: For your viewing pleasure take a look at these plates and tell me what you see

The first plate its pretty much spread throughout but the second one its mostly concentrated between 10-3 o'clock. On a plate like that the only place I would transfer from is 6 o clock.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I see it well on those. I was asking about the full plate I posted.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Well I will make a clone plate tomorrow and transfer it at least once. I'll even make a super duper cool thread where you all can tell me how wonderful my plate looks.
I will then either drop wedges in bags or go li, whichever would be suggested. As I will have bags prepped and sitting around you can pick your poison.
Dunno what else to try really
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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This
Quote:
36fuckin5 said: Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.
If those work it's your grain prep.
Or this
Quote:
hamloaf said: Dirty inoculm. Your stock cultures are dirty man. Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again. Trust me, I know how hard, and un motivating it is when you get a contam break out. Only thing that can be done, though is to clean everything out, and begin again. Sorry, maddchef. Things will get better. How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again. Good luck!
Anyone doing anything on a large scale will one day run into issues.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: azur]
#21798869 - 06/12/15 06:09 PM (8 years, 7 months ago) |
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Lol I'm nowhere even near hobby scale much less large. I just do more than I need with the knowledge imma have to throw most of it out.
Azur do you see any issue from cloning, is there ANY way mold could be intermingled in the actual tissue of the fruitbody?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
Edited by maddchef (06/12/15 07:23 PM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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You have a fruit on a contam'd sub? I personally wouldn't clone it unless that's all you've got. I'm about to send you a link.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: azur]
#21798906 - 06/12/15 06:15 PM (8 years, 7 months ago) |
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Nah its outdoors
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Outdoor fruits are always filthy
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: azur]
#21799135 - 06/12/15 07:11 PM (8 years, 7 months ago) |
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I consider any fruit to be filthy. It shouldn't affect the viability of the tissue sample though right?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
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I just wipe the outside of the stipe with rubbing alcohol, tear the stipe in half and cut a piece of tissue from the inside. The lower of the stipe the more aggresive. 99% success rate doing this method
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I would do that in a sab and not in front of your hood
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: azur]
#21799357 - 06/12/15 07:58 PM (8 years, 7 months ago) |
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Agreed or else I'll just blow shit all over my sample.
Damn now I gotta go find a cardboard box and some saran wrap
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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Quote:
maddchef said: Agreed or else I'll just blow shit all over my sample.
Damn now I gotta go find a cardboard box and some saran wrap
Sounds like you might have a few monotubs lying around not doing anything. Turn one on its side.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: Sksoul]
#21799577 - 06/12/15 09:05 PM (8 years, 7 months ago) |
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On its side? You suggesting I work through the non existent lid?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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invitro

Registered: 05/03/13
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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I've used cardboard boxes with success, but they are pretty flimsy after spraying and dusty if you don't spray enough. I guess you could tape foil to the cardboard and then spray the box down.
You can make arm holes in the lid of a monotub and tape em off when done or drape plastic wrap over the open side... Use a box if you want, I mean, things don't seem like they can get any worse. Good luck cleaning up your samples or getting cleaner ones!
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: Peace [Re: Sksoul]
#21800366 - 06/13/15 12:54 AM (8 years, 7 months ago) |
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If your agar's all fucked up, maybe try shooting some spores on grain... You do any edibles? Also check your pasteurization temps! slower and lower = better...
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ShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: Peace [Re: Sksoul]
#21800381 - 06/13/15 12:59 AM (8 years, 7 months ago) |
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100% success rate 0 contams baby!

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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Alright so today I cloned a fruit from a tissue sample, duh. Also made a few transfers to sectioned plates on half strength lme agar.
The thought is to turn the mushroom myc a bit more rhizomorphic and encourage it to grow outwards in search of food. I will likely make at least 2 more transfers but for now we will see how these few plates go.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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