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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21798460 - 06/12/15 04:47 PM (8 years, 7 months ago)

Well I will make a clone plate tomorrow and transfer it at least once. I'll even make a super duper cool thread where you all can tell me how wonderful my plate looks.

I will then either drop wedges in bags or go li, whichever would be suggested. As I will have bags prepped and sitting around you can pick your poison.

Dunno what else to try really


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleazur
God of Fuck
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: hamloaf]
    #21798771 - 06/12/15 05:49 PM (8 years, 7 months ago)

This

Quote:

36fuckin5 said:
Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.

If those work it's your grain prep.






Or this

Quote:

hamloaf said:
Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!




Anyone doing anything on a large scale will one day run into issues.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21798869 - 06/12/15 06:09 PM (8 years, 7 months ago)

Lol I'm nowhere even near hobby scale much less large. I just do more than I need with the knowledge imma have to throw most of it out.

Azur do you see any issue from cloning, is there ANY way mold could be intermingled in the actual tissue of the fruitbody?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Edited by maddchef (06/12/15 07:23 PM)


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Invisibleazur
God of Fuck
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Registered: 04/21/12
Posts: 28,103
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Re: Peace [Re: maddchef]
    #21798884 - 06/12/15 06:11 PM (8 years, 7 months ago)

You have a fruit on a contam'd sub?  I personally wouldn't clone it unless that's all you've got. I'm about to send you a link.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
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Re: Peace [Re: azur]
    #21798906 - 06/12/15 06:15 PM (8 years, 7 months ago)

Nah its outdoors


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisibleazur
God of Fuck
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Peace [Re: maddchef]
    #21799088 - 06/12/15 06:58 PM (8 years, 7 months ago)

Outdoor fruits are always filthy


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21799135 - 06/12/15 07:11 PM (8 years, 7 months ago)

I consider any fruit to be filthy. It shouldn't affect the viability of the tissue sample though right?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflinePinPornProducer
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Posts: 9,981
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Last seen: 6 years, 24 days
Re: Peace [Re: maddchef]
    #21799283 - 06/12/15 07:48 PM (8 years, 7 months ago)

I just wipe the outside of the stipe with rubbing alcohol, tear the stipe in half and cut a piece of tissue from the inside. The lower of the stipe the more aggresive. 99% success rate doing this method


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Invisibleazur
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Posts: 28,103
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Re: Peace [Re: PinPornProducer]
    #21799292 - 06/12/15 07:49 PM (8 years, 7 months ago)

I would do that in a sab and not in front of your hood


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: azur]
    #21799357 - 06/12/15 07:58 PM (8 years, 7 months ago)

Agreed or else I'll just blow shit all over my sample.

Damn now I gotta go find a cardboard box and some saran wrap


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineSksoul
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Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
Re: Peace [Re: maddchef]
    #21799506 - 06/12/15 08:40 PM (8 years, 7 months ago)

Quote:

maddchef said:
Agreed or else I'll just blow shit all over my sample.

Damn now I gotta go find a cardboard box and some saran wrap




Sounds like you might have a few monotubs lying around not doing anything. Turn one on its side. :grin:


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: Sksoul]
    #21799577 - 06/12/15 09:05 PM (8 years, 7 months ago)

On its side? You suggesting I work through the non existent lid?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Peace [Re: maddchef]
    #21800137 - 06/12/15 11:28 PM (8 years, 7 months ago)

:popcorn:


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OfflineSksoul
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Re: Peace [Re: invitro]
    #21800347 - 06/13/15 12:45 AM (8 years, 7 months ago)

I've used cardboard boxes with success, but they are pretty flimsy after spraying and dusty if you don't spray enough. I guess you could tape foil to the cardboard and then spray the box down.

You can make arm holes in the lid of a monotub and tape em off when done or drape plastic wrap over the open side... Use a box if you want, I mean, things don't seem like they can get any worse. Good luck cleaning up your samples or getting cleaner ones!


--------------------
Like all great travellers, I have seen more than I remember, and remember more than I have seen.


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InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: Peace [Re: Sksoul]
    #21800366 - 06/13/15 12:54 AM (8 years, 7 months ago)

If your agar's all fucked up, maybe try shooting some spores on grain...
You do any edibles?
Also check your pasteurization temps! slower and lower = better...


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


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OfflineShroominMe
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Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
Re: Peace [Re: Sksoul]
    #21800381 - 06/13/15 12:59 AM (8 years, 7 months ago)

100% success rate 0 contams baby!
:lolsy:


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: Peace [Re: ShroominMe]
    #21803834 - 06/13/15 09:03 PM (8 years, 7 months ago)

Alright so today I cloned a fruit from a tissue sample, duh. Also made a few transfers to sectioned plates on half strength lme agar.

The thought is to turn the mushroom myc a bit more rhizomorphic and encourage it to grow outwards in search of food. I will likely make at least 2 more transfers but for now we will see how these few plates go.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
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