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Invisiblemaddchef
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Re: Peace [Re: maddchef]
    #21796961 - 06/12/15 11:14 AM (8 years, 7 months ago)

I also have plates like this one which will one day "fuzz" up.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinehamloaf
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Re: Peace [Re: maddchef]
    #21796963 - 06/12/15 11:15 AM (8 years, 7 months ago)

Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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OfflinePinPornProducer
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Re: Peace [Re: maddchef]
    #21796968 - 06/12/15 11:16 AM (8 years, 7 months ago)

That plate looks like vert


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Invisiblemaddchef
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Re: Peace [Re: maddchef]
    #21796971 - 06/12/15 11:17 AM (8 years, 7 months ago)

I don't think the sammich will help with mold will it? I've transferred a million times, started over from spore, etc.

Tomorrow I will clone a fruit but I'm afraid it will ride along somehow. I just need a 100% clean culture so that I can concentrate on other variables.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinehamloaf
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Re: Peace [Re: maddchef]
    #21796988 - 06/12/15 11:21 AM (8 years, 7 months ago)

Take a transfer from said clone culture from the very leading edge of the culture BEFORE the culture hits the walls of the petri dish.  Taking a transfer no larger than a grain of rice from the leading edge of the culture gives the culture a window of opportunity to out run any contams may they be present.  Window closes once the myce hits the walls of the petris though.


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How I Get Stuff done. - My Reference Guide. - My Grows.


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InvisiblePastywhyteMDiscord
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Re: Peace [Re: hamloaf]
    #21796996 - 06/12/15 11:22 AM (8 years, 7 months ago)

Quote:

hamloaf said:
Dirty inoculm.  Your stock cultures are dirty man.  Time to ditch your spawn, clean your lab, get some new prints from a reputable dealer/vendor, and begin again.  Trust me, I know how hard, and un motivating it is when you get a contam break out.  Only thing that can be done, though is to clean everything out, and begin again.  Sorry, maddchef.  Things will get better.  How much better shit will get is going to be determined by your will & determination to get back up on your mycofeet, and begin again.  Good luck!




:whathesaid: Those all have mold meshed in riding along. Going to be really tricky to clean up. Like ham suggested I would start over from spores, use as few as possible.  Good luck.


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Invisiblemaddchef
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Re: Peace [Re: Pastywhyte]
    #21797002 - 06/12/15 11:24 AM (8 years, 7 months ago)

:frown:

Really depressing as I have already started from spore again and again run into this


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
Re: Peace [Re: maddchef]
    #21797013 - 06/12/15 11:27 AM (8 years, 7 months ago)

Would the fruitbody clone not be a better option or would it somehow mingle itself into the fruit itself?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflinePinPornProducer
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Re: Peace [Re: maddchef]
    #21797021 - 06/12/15 11:28 AM (8 years, 7 months ago)

Try making an sab of glovebox to do transfers in. Looks like you could salvage some clean growth from the second plate anywhere from 4 - 9 o'clock, just make a couple more transfers to be sure it's clean.
I throw everything I'm working with in this after wiping everything down with bleach water, give a quick spray of Lysol in the box, close it up and wait 10 min then go to work. I cut a little hole in the lower right side of the box for a flame sterilizing port. Just keep the flame a few inches away from the hole and sterilize the scalpel between transfers. This procedure works great for me


Edited by PinPornProducer (06/12/15 11:30 AM)


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Offlinehamloaf
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Re: Peace [Re: maddchef]
    #21797026 - 06/12/15 11:29 AM (8 years, 7 months ago)

Suck it up, and keep trying, man.  Failure is merely a stepping stone to success.  For some of us that stair well can have many steps.

Fruit body clone is great.  The best way to take a clone, imo, is from young pinhead.  Cloning from young pinhead generates the most vigorous culture due to the rate of expansion the mycelium is going through in a young pinhead.  Young pinheads are doubling in size every 24 hours.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisiblemaddchef
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Re: Peace [Re: hamloaf]
    #21797037 - 06/12/15 11:32 AM (8 years, 7 months ago)

Right but is there any chance for mold to piggyback if I take a core sample? I don't think that's possible but wanna make sure


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinehamloaf
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Re: Peace [Re: maddchef]
    #21797066 - 06/12/15 11:39 AM (8 years, 7 months ago)

There's always a chance of that.  Taking a core sample though virtually eliminates your chances of mold of bacteria spores piggybacking on your culture sample, as long as you are clean.  Just be as aseptic as possible.  You got this, maddchef!  Nothin' to it, but to do it!


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisiblemaddchef
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Re: Peace [Re: hamloaf]
    #21797078 - 06/12/15 11:41 AM (8 years, 7 months ago)

I've never seen mold intertwined with myc. I always see them bump up against each other and pretty much stop.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleMikeBearPig
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Re: Peace [Re: maddchef]
    #21797093 - 06/12/15 11:45 AM (8 years, 7 months ago)

As for a person who does everything as lazy as possible, I can tell you your vector is not your technique.

It is your inoculate..  I am convinced people confuse other aspects of their contamination with something that looks so convincingly clean. 

I agree with them, switch to jars and start fresh with something you have not made.  Maybe get a new syringe and go from there..


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InvisiblePastywhyteMDiscord
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Re: Peace [Re: maddchef]
    #21797096 - 06/12/15 11:45 AM (8 years, 7 months ago)

Frank had some real issues with meshed in mold a few years ago. It was in nearly all his cultures. Its not unheard of.


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Invisiblemaddchef
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Re: Peace [Re: MikeBearPig]
    #21797099 - 06/12/15 11:46 AM (8 years, 7 months ago)

On the full plate, will someone explain how to identify or how you know there is intermingled mold?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Re: Peace [Re: maddchef]
    #21797170 - 06/12/15 12:02 PM (8 years, 7 months ago)

Its usually thin and tomentose looking. In the thinnest parts look carefully to see if the myc is organised or random.


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Invisiblemaddchef
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Re: Peace [Re: Pastywhyte]
    #21797172 - 06/12/15 12:03 PM (8 years, 7 months ago)

I don't see it


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Peace [Re: maddchef]
    #21797226 - 06/12/15 12:18 PM (8 years, 7 months ago)

Quote:

maddchef said:
For your viewing pleasure take a look at these plates and tell me what you see





The first plate its pretty much spread throughout but the second one its mostly concentrated between 10-3 o'clock.  On a plate like that the only place I would transfer from is 6 o clock.


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Invisiblemaddchef
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Re: Peace [Re: Pastywhyte]
    #21797802 - 06/12/15 02:25 PM (8 years, 7 months ago)

I see it well on those. I was asking about the full plate I posted.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
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Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag


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