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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Peace
#21796481 - 06/12/15 08:58 AM (8 years, 7 months ago) |
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Fuck it all to hell. I just saw green in a bag, again. I don't know how to be any more careful, I really don't. I have gone over and over my technique.
The bags are run long enough to kill mold so that's not it. My bags are opened the shortest amount I can get away with and inoculated with what appears to be 100% clean wedges.
I've tested my hood by opening a plate, facing it towards the filter and moving it over every inch so if there was a leak it would be easily detected, the plate stayed clean.
This used to be a fun hobby, used to. Now I'm all fucking stressed out because everything I do I have to throw away.
I'm out.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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That's nice dear.
Maybe stop using bags?
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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still air box, pp5, whole brown rice, LI/LC, coir/verm.
You seem to have been over everything, but you are clearly missing something. Try smaller jars (pints or even half pints) and noc up a shit ton of them... Keep it simple til you get the results you are looking for.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: Sksoul]
#21796587 - 06/12/15 09:33 AM (8 years, 7 months ago) |
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I didn't have any better results with jars. It's either something I'm mistaking for mycelium on my plates or I'm just ignorant.
It just happened one day out of the blue and now I can't grow shit.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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How much success have you had with bags in the past? I suggest switch to jars for a single grow. That way you at least narrow the vector.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I had very limited success with jars before I switched if you remember.
But I could never nail down the cause. It would kinda come and go but I'm not the best at tracking my cultures. Although I scrapped every culture I was working with and went back to spore->agar.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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pc issue? Have you left jars or bags sealed after pc'ing to see if they get contaminates growing in them?
If it isn't your grain prep, your sterilization, or your culture then its how you are inoculating. It has to be one of those. That's why I suggested lots of tiny jars. You can test a ton of variables in a short time.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Peace [Re: Sksoul]
#21796699 - 06/12/15 10:00 AM (8 years, 7 months ago) |
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It's either something I'm missing on my plates or my technique.
I dunno what else to do for my technique. I've worked commercially in a hood and am quite familiar with usp 797 and aseptic techniques.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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I wanna say its not my technique since all of my agar work is clean ie: no bacteria or colonies
But I wanna say its not my cultures because I always use agar and it always looks exceptional.
Fucked if I know
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Sksoul
Pan handler



Registered: 10/31/14
Posts: 397
Loc: Far East
Last seen: 3 years, 6 months
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Even if your technique was shit, you'd probably not have as many problems as you do. I g2g using ziplocks in a sab, fer fucks sake and don't have contam issues at all.
I doubt you could have so many crap cultures, though... clean growth over a few transfers is enough. Now I'm stuck. Wanted to help you troubleshoot, but sorry buddy.
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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yup, switch to jars and see if that helps.
and run through your sterile technique to make sure you arent using any short cuts (such as reusing old gloves)
-------------------- (Yes, the egg is real)
How to post pics
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Been through the same thing with jars
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Try some PF cakes. Dry everything in the oven to be sure it's all at the perfect water content when you hydrate.
If those work it's your grain prep.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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For your viewing pleasure take a look at these plates and tell me what you see
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Those are not clean plates man
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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First one looks good, second has a spot of bacteria at about 8 o'clock.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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2nd plate looks fkd from 10 - 3 And the first plate doesn't look right to me either but
Edited by PinPornProducer (06/12/15 11:09 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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The second plate came from the leading edge of the first plate before "it" grew out.
Yeah neither are good. The second plate has a piece I could grab from but this is what is piggybacking all my cultures and I cannot seem to get rid of it.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Tried the agar sandwich yet?
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