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Offlinetrg64
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oyster or cobweb mold?
    #21795992 - 06/12/15 04:00 AM (8 years, 7 months ago)

just to confirm what I think..
In addition it smells more cheesy than anis..





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OfflineYerow
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Re: oyster or cobweb mold? [Re: trg64]
    #21795999 - 06/12/15 04:06 AM (8 years, 7 months ago)

Oyster mushroom mycelium usually starts out very whispy and kinda looking like cobweb IME, give it a day then take a look.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: Yerow]
    #21796005 - 06/12/15 04:12 AM (8 years, 7 months ago)

it's been growing slowly over the last weeks, no explosion whatsoever, so it might as well be mycelium then???

I'm wondering because I think about transfering some to a petri dish..


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21796949 - 06/12/15 11:11 AM (8 years, 7 months ago)

That looks like Oyster myc to me but if its taking weeks to grow something may be wrong. Usually my oysterer myc is fast and aggressive. In my last project 5 gallons of coffee grounds were fully colonized in 5 days. How much spawn did you use? Is your straw properly hydrated? And finally what kind of container are you using? Myc likes high co2 buildup.


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OfflineGr0wer
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21797219 - 06/12/15 12:16 PM (8 years, 7 months ago)

I agree with above posts. I too was scared when i grew my first oysters, nothing like cube dense rhizomorphic growth, although sometimes it will grow dense rhizomorphs.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: Gr0wer]
    #21797934 - 06/12/15 02:56 PM (8 years, 7 months ago)

not so surprising to me: I got 3 dowels from a friend that i put in this jar with wet cardboard and loose straw. Here we are now after a bit less than 2 weeks.

So i'm not tossing it, cool ;-)

I'll try to put it on dog agar to see if I can start some grains with it later..


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21798200 - 06/12/15 03:58 PM (8 years, 7 months ago)

put in on agar for sure. keep us updated on how it goes.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21801837 - 06/13/15 10:40 AM (8 years, 7 months ago)

so here it is two days later.. What do you think?





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Offlineknomadic_niki
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Re: oyster or cobweb mold? [Re: trg64]
    #21802476 - 06/13/15 01:02 PM (8 years, 7 months ago)

it looks like oyster mycelium to me but your straw is not packed tight enough. soak a piece of cardboard the size of the 5 gal bucket diameter and place it on top, then put a rock on it to weigh it down.


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: knomadic_niki]
    #21803808 - 06/13/15 08:51 PM (8 years, 7 months ago)

its myc for sure. I still can't see the container its colonizing in but one reason it may be colonizing slowly is the shape of the container. Myc tends to stall in taller jars.



if its colonizing in something like this you  aren't getting enough GE, especially if the lid is sealed.


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Offlineknomadic_niki
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21804112 - 06/13/15 10:51 PM (8 years, 7 months ago)

oh, i just assumed it was a 5 gal bucket....i still think it needs to be more compressed. too much space between the pieces of straw for the myc to get dense


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: knomadic_niki]
    #21804351 - 06/14/15 12:04 AM (8 years, 7 months ago)

Quote:

knomadic_niki said:
oh, i just assumed it was a 5 gal bucket....i still think it needs to be more compressed. too much space between the pieces of straw for the myc to get dense




I agree :thumbup:


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21804735 - 06/14/15 02:14 AM (8 years, 7 months ago)

so I'm doing agar today, and then transfer it to a new container with more pasteurized straw, more tight, more hole for FAE


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OfflineYerow
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Re: oyster or cobweb mold? [Re: trg64]
    #21804936 - 06/14/15 04:28 AM (8 years, 7 months ago)



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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: Yerow]
    #21806056 - 06/14/15 12:13 PM (8 years, 7 months ago)

Are you transfering agar>straw or colonized straw>more straw?

Once your agar dish colonizes transfer agar>grain. Then do gran>grain to expand your spawn. 

put a drop of red food coloring in your agar if you can. I just started doing this. Makes it way easier to see whats going on.


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21806130 - 06/14/15 12:24 PM (8 years, 7 months ago)

Quote:

trg64 said:
so I'm doing agar today, and then transfer it to a new container with more pasteurized straw, more tight, more hole for FAE




Hey man I'm not trying to be a downer but the more clear you are with your posts the more we will be able to help. We want you to succeed!

Will you be transferring the colonized straw into a bucket of uncolonized, pasteurized straw? Colonizing mycelium does not like fresh air exchange. It prefers high C02 buildup, so if you do drill holes in your bucket be sure to loosely wrap it with a garbage bag. If my bucket is new (no holes) I let it colonize with the lid on losely, then I drill the holes once colonization is complete. The drill bit hardly damages the myc, Oyster is forgiving and doesn't seem to mind :smile:

The reason the lid is on loosely, or in your case loosely wrapped with a bag is to provide gas exchange. Looking up the difference between FAE and GE will help you understand this process better!


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21806191 - 06/14/15 12:39 PM (8 years, 7 months ago)

ok, yeah I didn't say much, sorry..

here is what I did:

Colonized straw to pasteurized straw in 750mL jars with two small holes covered with micropore tape:



and just before that I transfered a bit of mycelium on dog food agar in two different petri dishes in a SAB. It is probabely not the best since it comes from a definitely not sterile environement, but It's just to see what is going to happen..


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21806197 - 06/14/15 12:41 PM (8 years, 7 months ago)

by the way, what does GE stands for? (so many SAB, FAE, FC, PC, ... to learn :-D


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21806380 - 06/14/15 12:52 PM (8 years, 7 months ago)

:facepalm3: Would have been better to transfer a chunk of colonized straw to a larger container of uncolonized straw. Straw will never colonize in those jars. It seems like you are trying to apply the PF tek method to bulk methods.

If you want your spawn to colonize in jars like that use brown rice flower, birdseed, or rye. Any of these things when colonized are referred to as spawn.

Your spawn can be mixed in with bulk substrates like staw, manure, woodchips, for fast colonization.

GE is gas exchange. FAE is fresh air exchange. two very different things.

Don't sweat the agar. Thats what its for. People take fruits from the unsterile outdoors and clone them all the time. You will just need to tranfer a chunk of colonized agar on to a fresh dish 2 or 3 times until it is clean.

Good luck.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21806609 - 06/14/15 01:56 PM (8 years, 7 months ago)

ok, I did transfer colonized straw into uncolonized straw in these jars.
What do you mean by a larger container of uncolonized straw?

I tried to do something like this but instead of polytubing i used the jars and I thought colonized straw could replace colonized grain..


I'd be happy for you to correct me because I'm lost now :confused:


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Edited by trg64 (06/14/15 01:57 PM)


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21806626 - 06/14/15 02:01 PM (8 years, 7 months ago)

to make it clear, they are not syringe inoculated, it is just the containers I had where I could compact the straw. We can't see but there are colonized dowels (1 or 2), colonized pieces of cardboard and colonized chunks of straw inside.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21806646 - 06/14/15 02:10 PM (8 years, 7 months ago)

ah ok psilociben, I read your posts from the beginning, if I understand correctly the problem here is the jars are too tall, right?


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21807501 - 06/14/15 06:22 PM (8 years, 7 months ago)

You got it brother. It is likely that high C02 buildup will cause them to stall. Myc likes co2 during colonization, but still needs oxygen because its a living thing! Thats what people mean when they refer to gas exchange. Think of it like the mycelium breathing.

Best thing to do is get your agar started. I'm on my mobile right now but i'll write you a more detailed note on how to procceed when I get home.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21835647 - 06/21/15 06:49 AM (8 years, 7 months ago)

yep, it stalled !

so my last bit of oyster is on agar, and It definitely needs to be purified :eek:
got a project :laugh:


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: trg64]
    #21841542 - 06/22/15 12:38 PM (8 years, 7 months ago)

Good attitude. :thumbup: If you keep this thread rollin I'm happy to monitor it encase you run into any troubles.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21841748 - 06/22/15 01:53 PM (8 years, 7 months ago)

oh yes sure, kind offer :thumbup:
Here is the agar plate after transfer


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Edited by trg64 (06/22/15 01:54 PM)


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Offlineknomadic_niki
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Re: oyster or cobweb mold? [Re: trg64]
    #21846850 - 06/23/15 05:26 PM (8 years, 7 months ago)

ooooooh, that's not good


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: knomadic_niki]
    #21848976 - 06/24/15 05:07 AM (8 years, 7 months ago)

yeah knomadic, but it is nice :rolleyes:

the tranfers I did from this one seems to be going well (pic not good, and the second petri dish looks the same)


and this is the sister of the multicolor dish in the previous post, which I'm about to transfer to 5 grain jars obviously inoculated with a dead spore syringe. (I know it is not the best but as you can see I'm not afraid to learn from my mistakes, I'm going away for 10 days, and I prefer to do that instead of just tossing the jars :wink:)


oh and I've seen a small bacteria inside the ring close to 6:30, I conclude that is why there is a small discontinuity in the shape of the ring there, so I'm going to avoid this area, and take triangles enclosing the leading edge of the myc..


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Edited by trg64 (06/24/15 05:12 AM)


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OfflinePsilocyBen17
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Re: oyster or cobweb mold? [Re: knomadic_niki]
    #21850158 - 06/24/15 12:07 PM (8 years, 7 months ago)

Quote:

knomadic_niki said:
ooooooh, that's not good




I dont see what the problem is? He transferred unsterile myc > sterile agar. Of course it will need cleaning up! thats what agar is for.

Looking good TRG. You are learning fast. Where did you get your petris? I wish I had some glass ones that I could PC, but they are expensive. I use small 250ml mason jars.

I put a few drops of red food colouring in my last batch of agar (PastyWhites idea) to help see myc growth better. Try it next time just for kicks.


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Re: oyster or cobweb mold? [Re: trg64]
    #21850165 - 06/24/15 12:10 PM (8 years, 7 months ago)

Quote:

trg64 said:


and this is the sister of the multicolor dish in the previous post, which I'm about to transfer to 5 grain jars obviously inoculated with a dead spore syringe. (I know it is not the best but as you can see I'm not afraid to learn from my mistakes, I'm going away for 10 days, and I prefer to do that instead of just tossing the jars :wink:)






PC them for 90 minutes and let them cool before knocking them up with the agar. There might be all sorts of stuff in there by now...


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: PsilocyBen17]
    #21850408 - 06/24/15 01:08 PM (8 years, 7 months ago)

too late
it's cheap glass petri boxes, 1euro each on an internet chemist supply shop.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21896231 - 07/04/15 10:49 AM (8 years, 6 months ago)

just came back today, here is the only straw jar I didn't toss, it smells fresh although I think the anis smell could be more present:


It is funny how the smell was not so good at the beginning and changed as it was colonizing.

the old grain jars reinoculated with agar. I'm keen on comments on that since it doesn't look homogeneous:


these are jars that were sterilized just before being inoculated, maybe a bit wet, with signs of wet spots bacterial contam (the third one just been shaken:

I wonder how bad it is for the mushrooms if they fruit and for consumtion? What if the myc overpowers it?
Any information on the subject would be ingested, analyzed, and remembered :takingnotes:

hope you're all well guys :crazy2:


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Edited by trg64 (07/04/15 01:43 PM)


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Invisiblemushme
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Re: oyster or cobweb mold? [Re: trg64]
    #21896803 - 07/04/15 01:45 PM (8 years, 6 months ago)

Those look good to me, might be a pain to break up if you dont shake those jars soon.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: mushme]
    #21896828 - 07/04/15 01:51 PM (8 years, 6 months ago)

sweet, i'm up to that right now ^^


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21909380 - 07/07/15 11:49 AM (8 years, 6 months ago)

so here is the update:

the batch of grain jars, some just shaken some not, the two on the top left are not oyster..

and what I dit with two of them:


I am not sure how it is going to turn, the smell was a bit sour.. maybe caused by the fact that the jars were 1 month old when I inoculated? shame, we will see..


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21917893 - 07/09/15 02:44 AM (8 years, 6 months ago)

hi,

I got these jars still on the shelf, 3 out of 4 have some wet spot inside.. I don't know how bad it is?? I was thinking to maybe wait to see how i becomes if I wait to see if the myc takes over, or decides to eat it if it can?? Maybe try to fruit them outside later??


Two good looking ones I try to fruit this way, with the straw one I put in there 4 days ago (that i dunked before I learned it was not really necessary for oyster).


Any comment or remarks about anything?


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21917896 - 07/09/15 02:46 AM (8 years, 6 months ago)

Psilociben !! Whare are you? I miss you in here :smirk:


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OfflineYerow
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Re: oyster or cobweb mold? [Re: trg64]
    #21919946 - 07/09/15 01:04 PM (8 years, 6 months ago)

Remember oysters require lots of fae and make sure to mist if the substrate is dry


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: Yerow]
    #21920476 - 07/09/15 03:27 PM (8 years, 6 months ago)

ok, I will do the misting. So if I understand well I should not put any more jars in there. For Fae I suppose what I can do is fan a lot for the moment as long as I don't have a real fruiting chamber or a small greenhouse to keep the conditions correct.

Do you think I need to prepare myself to observe that the FAE is not enough?


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Offlinebryanbzl
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Re: oyster or cobweb mold? [Re: trg64]
    #21923242 - 07/10/15 06:16 AM (8 years, 6 months ago)

I will not say it will not work. But doing straw in jars seems like going against everything straw is about. If you need to multiply the amount of spawn you need then do grain to grain transfers (g2g). once you have the spawn you need - spawn your grains to bulk pasteurized straw. Hope this helps.


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: bryanbzl]
    #21924998 - 07/10/15 03:16 PM (8 years, 6 months ago)

hi brian

yeah now I know about the straw jars. I have two straw bags i did following RRs vids. For grain to grain, I'm gonna wait to have jars with no wet spot in it. To recap this is my first agar to grain, first grain, first straw bags, and first fruiting to come I hope. The straw jars came from 3 dowels put on cardboard and loose straw that gave me the myc to do my first agar work, with agar transfer to clean the culture, and the myc and straw went into these jars just to see what would happen, the main stuff is these grain jars and now straw bag..

but I'm still curious to see if the only straw jar that didn't stall is going to fruit ;-) And to see if a grain jar can fruit..


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Offlinetrg64
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Re: oyster or cobweb mold? [Re: trg64]
    #21936595 - 07/13/15 09:49 AM (8 years, 6 months ago)

a little update because I'm not sure if I should do something with these grain cakes:


I mist and fan about twice a day, I didn't dunk them neither coat with vermiculite.. Should I? They have been in the terrarium for 3 or 4 days now, the myc has been expanding as you can guess, and yeah I don't know what to expect or how to judge if all is going good..

thanks if u can help me again :takingnotes: :grin:


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Edited by trg64 (07/13/15 11:38 AM)


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