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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Pan Cyan Hawaii first time
#21793197 - 06/11/15 02:08 PM (8 years, 7 months ago) |
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I've been slowly thinking about trying Pan Cyans for a while. Had a syringe in the drawer for a while now also. I mathed it and it finally added up considering the temps are becoming too hot for cubes this season. In a SAB I knocked up 3 quarts of rye and 3 quarts of LC about a month ago. I wasn't sure how I wanted to proceed, but I assumed anything I wanted to do I could do with those items one way or the other.
The ryejars performed horribly. Slow slow weak growth. (Picture coming soon)
The LCs did fine. I put one in the fridge for storage, one is still sitting out vegetating and the other I pulled 30 CCs from. Afterwards I opened up the jar and it smelled amazing.
 

A week ago I put the drawn LC on 10 plates to test. I did this with the last syringe I drew (thinking it was going to be the dirty of the three. 9 out of 10 plates came back clean. One came back with trich. I assumed I was clear to proceed. Pan Cyan grew extremely slow on both my MEA and rye.
Clean plate

Trich

I was studying some of blue helixs techniques. I'm not sure about Agars myco-nitro compost but I just substituted hpoo for it. By dry weight I added.... 70% hpoo 20% rye berries 10% straw

Plus I added a few handfuls of verm and a few tablespoons of gypsum. I wasn't a fan of throwing in all these ingredients some dry and some wet together so I individually brought all to proper moisture content seperately. Straw was thrown in soap water for 2 hours. Rye was simmered for 15 minutes and hpoo was brought up to slightly drier than field capacity. All was mixed and thrown into a spawn bag and PCed for 3 hours at 15 psi.

I knocked up the bag in a SAB with all 10 CCs of solution.
I'll update as she comes along. If all goes well I'll probably do 10 or so bags before long.
Any ideas on the slow growth anyone?
Edited by RyeJar (06/12/15 02:39 AM)
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unseenat17
Midnight Toker



Registered: 11/28/05
Posts: 419
Last seen: 8 years, 7 months
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Re: Pan Cyan Hawaii experiment [Re: RyeJar]
#21795135 - 06/11/15 09:44 PM (8 years, 7 months ago) |
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I tried years ago and never succeeded. Hope it goes well
--------------------
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nobody83
stranger danger


Registered: 03/15/14
Posts: 1,486
Loc: around town
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Re: Pan Cyan Hawaii experiment [Re: unseenat17]
#21795225 - 06/11/15 10:08 PM (8 years, 7 months ago) |
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Pan Cyan Hawaii experiment [Re: nobody83]
#21796728 - 06/12/15 10:07 AM (8 years, 7 months ago) |
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Adding more grain into your bulk won't help. Next time use verm instead of the rye and that'll be an awesome mix.
Pans are just slow IME. Nothing to worry about.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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Re: Pan Cyan Hawaii experiment [Re: 36fuckin5]
#21797347 - 06/12/15 12:46 PM (8 years, 7 months ago) |
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I was doing a lot of searching on pans and blue helix seems to have had the best writeups so I went with them even though they are old. I was curious about why he added wbs to his mix. But yes I definitely added a good amount of verm to when I saw the final mix. Also blue helix is always going on about how fast pans are. He gets fruits he claims 3 weeks after lc to substrate. Is this just old info? Is there a better updated tek for pans I missed? Thanks for the info bro.
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mycomaniac1402
Myco Maniac :-)



Registered: 01/25/15
Posts: 257
Last seen: 2 months, 19 days
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Re: Pan Cyan Hawaii experiment [Re: RyeJar]
#21797532 - 06/12/15 01:20 PM (8 years, 7 months ago) |
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Try adding Manure water to your petri or Lc.
They definitely are quick when on manure. On manure Pf cakes they are so so but as soon as they touch air and manure after spawned, they swell and puff up quickly as the mycellium spreads.
I stay with simple substrates when I do pans like Manure soaked straw and Vermiculite.. see signature pics. And crumbled Manure pf cakes as spawn . I haven't gone grain with them yet. Always stuck with what worked the first few runs for some reason. Also I need a pressure cooker like I used to have as picking up the hobby once again so I resort to PF cake spawn for everything .
And spore prints from a couple months ago have germinated on Modified PF cakes in 3 days compared to a stored old print in 7 to 10. I really need a good pc, making sets of 8 half pints in two separate pots and steaming really is a pain in the ass! But If I did grain again I would have more stuff yo spawn than I could handle..
Edited by mycomaniac1402 (06/12/15 01:23 PM)
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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I screwed up the math when doing the LC. Only put 2 grams total lme and dextrose in the solution. They seemed to do great haha. I have thought about adding poo water into my agar. I'm going to do some transfers with those plates today or tomorrow so that's something to think about.
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mycomaniac1402
Myco Maniac :-)



Registered: 01/25/15
Posts: 257
Last seen: 2 months, 19 days
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Re: Pan Cyan Hawaii experiment [Re: RyeJar]
#21797870 - 06/12/15 02:43 PM (8 years, 7 months ago) |
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I have seen them do alot better with manure in my Lc instead of plain water & when using spring water I definitely see a difference in speed compared to my chlorinated tap water..
Are they slow on grain also? Obviously your using agar wedges insteas of spores to grain so that is surprising but like I said , anything including cubes put to manure seems to do really well. Ive even used left over manure in my pf jars for cubes and they tore right through it. I think its better to leach the manure nore though if going for manure added a pf jar... Just so its not so rich
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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I actually went spores to LC (I read isolating pan cyans is usually not required for performance) I just used agar plates to test the cleanliness of the LC before using it. Since I got so many clean though I will try to start isolating and testing for performance just for fun.
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mycomaniac1402
Myco Maniac :-)



Registered: 01/25/15
Posts: 257
Last seen: 2 months, 19 days
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Re: Pan Cyan Hawaii experiment [Re: RyeJar]
#21797946 - 06/12/15 03:00 PM (8 years, 7 months ago) |
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I heard the same , that its a bigger feat to find a good performing culture for whatever reason from spores... I talk with a few experienced Pan cult on a regular basis that have been working with them longer than I have that all say its better to go from clone then isolate further to get your culture thatll preform the way you want. So starting from spore to clone then isolate seems like the most logical route.
I have about ten successful go`s at Pans and love the tempermental nature along with the strength of the fruits "different feeling for sure"
And since ive got to know them I kinda lost my interest in cubes but I still like the ease of making a tub here and there for fun. But I mostly have been learning how to play with this species,they keep my interest a bit better.
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RyeJar
StrangerDanger


Registered: 04/16/15
Posts: 523
Last seen: 9 months, 30 days
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I am super excited to see and try these little buggers. Cubes are great but I'm ready to move on to more temperamental strains.
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