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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Ran out of inoculant
#21793041 - 06/11/15 01:31 PM (8 years, 7 months ago) |
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Since I let myself run out of plates I am also out of inoculant. But I have almost mature fruits........
Am I stuck cutting sections out of the interior for a slurry?
I remember back in the day someone used to slurry with whole fruits but that is just asking for trouble.
Anyone have any suggestions or a lesser known method they use?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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thebitterbuffalo26
Fartyr



Registered: 04/18/15
Posts: 555
Loc: Texas
Last seen: 7 years, 7 months
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Re: Ran out of inoculant [Re: maddchef]
#21793050 - 06/11/15 01:33 PM (8 years, 7 months ago) |
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Uh make more plates?
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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My sleeves won't be here for another 2 days.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Ran out of inoculant [Re: maddchef]
#21793130 - 06/11/15 01:53 PM (8 years, 7 months ago) |
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Make a few no pour plates. Work great, nothing to order.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Ran out of inoculant [Re: maddchef]
#21794426 - 06/11/15 06:47 PM (8 years, 7 months ago) |
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Quote:
maddchef said: Since I let myself run out of plates I am also out of inoculant. But I have almost mature fruits........
Am I stuck cutting sections out of the interior for a slurry?
I remember back in the day someone used to slurry with whole fruits but that is just asking for trouble.
Anyone have any suggestions or a lesser known method they use?
you can take stem tissue and blend it into sterile water just like an LI. It catches a little slower on grain. it catches much faster on pf cakes. i suck it up into syringes and inject a pf cake or a quart grain jar. the longest its taken to colonize a quart jar of stem tissue for me was about 12 days. a pf jar will colonize in 4 days with stem tissue if you use about 5cc per 1/2 pint jar.
or you can cut some inner stem and put it on a plate. actually i read recently this kid had slurried jars that had large pins on them and had successful grows. ive been meaning to try it for shits and giggles. but i never let my jars colonize invitro. ive let jars sit for weeks and they won't even pin. but i do keep them in a dark ass cabinet.
yeah no pour is awesome. you can go to the store and thats all you need! i hate ordering supplies. i like DIY grocery store teks the best!
Edited by eatyualive (06/11/15 06:49 PM)
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Ran out of inoculant [Re: eatyualive]
#21794437 - 06/11/15 06:48 PM (8 years, 7 months ago) |
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Yeah imma just tissue sample slurry the mofo. It's gonna be a thin solution though I'm afraid
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Ran out of inoculant [Re: maddchef]
#21794773 - 06/11/15 08:13 PM (8 years, 7 months ago) |
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ShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: Ran out of inoculant [Re: eatyualive]
#21794849 - 06/11/15 08:31 PM (8 years, 7 months ago) |
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Even if your syringe is empty there are still millions of spores in there. You might try sucking up some sterilized distilled water into the syringe and using that. All in a sterile environment, of course.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Ran out of inoculant [Re: ShroominMe]
#21794909 - 06/11/15 08:46 PM (8 years, 7 months ago) |
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He is working with culture and probably doesn't care to go back to spores right now. When you're rolling with culture you tend to have a few solid clones on hand and prefer to inoculate with live tissue.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Ran out of inoculant [Re: Pastywhyte]
#21794948 - 06/11/15 08:55 PM (8 years, 7 months ago) |
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Quote:
Pastywhyte said: He is working with culture and probably doesn't care to go back to spores right now. When you're rolling with culture you tend to have a few solid clones on hand and prefer to inoculate with live tissue.
tissue is king!
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Ran out of inoculant [Re: eatyualive]
#21795065 - 06/11/15 09:25 PM (8 years, 7 months ago) |
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Yeah I have a 60cc spore syringe laying around and my masters but I don't have any disposable plates atm.
I may just use 2 or 3 tissue samples from multiple fruits for slurry. I reckon my li blender will do fine, just hate that it won't be thick. Maybe I'll just make an offer out of it. Throw in some lme, blend, and wait two days.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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