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Offlinexxbigmilkxx
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Flow hood types horizontal vs vertical
    #21792955 - 06/11/15 01:10 PM (8 years, 7 months ago)

Witch style flow hood is better vertical or horizontal for mycology work I am buying not building mine if that means anything


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Invisiblemaddchef
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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793107 - 06/11/15 01:48 PM (8 years, 7 months ago)

Horizontal really but honestly as long as you know what you're doing either will work but if you have the choice go horizontal.

Also can I borrow some monies?


--------------------
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Offlineblojo02184
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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793116 - 06/11/15 01:50 PM (8 years, 7 months ago)

So, need to define a little bit better.

Horizontal flow or vertical flow?
Or
Horizontal filter face or vertical filter face?

Buying is expensive compared to building. Fyi.

But, ill gove more info onve you respond.

If youdecide to build check my sig for the flow bench math already worked out.


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Offlinexxbigmilkxx
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Re: Flow hood types horizontal vs vertical [Re: blojo02184]
    #21793135 - 06/11/15 01:53 PM (8 years, 7 months ago)

A horizontal facing filter compared to a over head vertical facing filter


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Offlinexxbigmilkxx
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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793141 - 06/11/15 01:54 PM (8 years, 7 months ago)

Also can any one explain the pros and cons of each


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InvisiblebodhisattaMDiscordReddit
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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793215 - 06/11/15 02:11 PM (8 years, 7 months ago)

vertical = not great for mycology, requires impeccable sterile technique, your hands can never be above your work(shouldn't ever anyway), there's turbulent wash as it hits the bottom all of your work needs to be elevated off of the floor. Great for lab work were complete sterility isn't needed, most agar work done in a V-FH is for specific organisms on specific media, absolute sterility isn't a must, but they're way better than the bunsen burner ways that labs that cant afford a hood use.

Horizontal = good for mycology work

Cons, for both types is you have to let them run at least 15m before using them, and you have to have a dedicated semi-clean room to put it in. you can't work with contaminated plates at all since you're working with moving air you have to start clean and stay clean, there's no cleaning up cultures with a hood. And the biggest con, doesn't magically make you better at sterile technique but actually requires impeccable sterile technique.

the only real pro of a horizontal FH for mycology work is increased work space.

Still air box. = pro. you can build it bigger to have just as much work space and arm room clearance. you don't have to wait 15m to use it. you can go barehanded if you want to, you can work with contaminated cultures. 1/100 the cost. also you can have a little bit of wiggle room on sterile technique
cons: not as cool


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Offlinexxbigmilkxx
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Re: Flow hood types horizontal vs vertical [Re: bodhisatta]
    #21793237 - 06/11/15 02:15 PM (8 years, 7 months ago)

any good links on sterile work with a flow hood


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InvisiblebodhisattaMDiscordReddit
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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793250 - 06/11/15 02:18 PM (8 years, 7 months ago)

practice. I could show you how to ride a bike videos but it doesn't teach you how to ride a bike...

there's hundreds of videos of people using flow hoods, you can watch them to pick up on things but it will never ever make up for experience.


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Offlinexxbigmilkxx
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Re: Flow hood types horizontal vs vertical [Re: bodhisatta]
    #21793273 - 06/11/15 02:23 PM (8 years, 7 months ago)

i have plenty of practice with agar and still air boxes but im wanting to take it to the next step with a flow hood and sterile work so i can eliminate all contams before fruiting im going to be doing alot of agar work and cloning and slurry work i just figured having a flow hood instead of doing everything in a still air box would be benifical for me would you agree


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Re: Flow hood types horizontal vs vertical [Re: xxbigmilkxx]
    #21793332 - 06/11/15 02:38 PM (8 years, 7 months ago)

nope if you can't make it to 99% success with a SAB then a FH is not for you, because it won't magically help.

if you're really awesome with a SAB but it's to the point where you're making so many jars that it's prohibitive to keep on loading and unloading your SAB full of jars and plates that's when you need a FH.

A SAB can enable you to eliminate all contams.

Some people buy a 1500 dollar guitar when they can only play ACDC songs. whatever floats your boat but if you can't use it to the potential then I say it's a terrible investment.

if you can afford it though, who cares, it's not my money.
Some people learn to drive on an automatic and some people learn to drive stick right off the bat. either way neither is wrong. but using a FH will make you learn quick.


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Re: Flow hood types horizontal vs vertical [Re: bodhisatta]
    #21793347 - 06/11/15 02:43 PM (8 years, 7 months ago)

I haven't put my hands in a hood since I was in college but I've never thought I needed one for mycology work.


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