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Peaceful Soul
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Oat grain - is this jar ready to spawn?
#21789411 - 06/10/15 05:31 PM (8 years, 7 months ago) |
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Does it get any better or is this just what 100% collonised oat grains looks like?
Jar is 2 weeks old

Edit: Same jar, better lightning:
Edited by Peaceful Soul (06/10/15 05:36 PM)
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Grim767
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21789429 - 06/10/15 05:35 PM (8 years, 7 months ago) |
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That doesn't look near enough to be ready to me but I've never used oats before
-------------------- Trade List I won't bow to something that I've never seen, Can't believe in something that doesn't believe in me, I'm not blood of your blood, I'm no son of your god, I have no faith in your faith, Still I find salvation.
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: Grim767]
#21789453 - 06/10/15 05:41 PM (8 years, 7 months ago) |
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It doesn't look good. To me it looks bacterial.
Edited by Mad Season (06/10/15 05:42 PM)
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maddchef
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21789460 - 06/10/15 05:43 PM (8 years, 7 months ago) |
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Your jar says ms->lc? Spore syringe to lc?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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DetectiveLefty
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Re: Oat grain - is this jar ready to spawn? [Re: Grim767]
#21789469 - 06/10/15 05:45 PM (8 years, 7 months ago) |
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Let it go a few more days- Ima say you shook that jar a few days ago at about 10% colonization. Should be pretty much completely white when it's 100%, assuming that (wild guess) you're growing P. cubensis.
Oats?!!! What are you, in Scotland?
-------------------- I swear on a stack of Bibles that everything I said in the above post is the 100% literal Truth.
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: maddchef]
#21789477 - 06/10/15 05:47 PM (8 years, 7 months ago) |
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Quote:
maddchef said: Your jar says ms->lc? Spore syringe to lc?
Jar says: Mc - LC ( Mckinney - Liquid culture) The LC was made out of a homemade sporeprint :-)
Edited by Peaceful Soul (06/10/15 06:00 PM)
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21789493 - 06/10/15 05:50 PM (8 years, 7 months ago) |
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Looks like yeast to me
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: DetectiveLefty]
#21789509 - 06/10/15 05:55 PM (8 years, 7 months ago) |
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Quote:
DetectiveLefty said: Let it go a few more days- Ima say you shook that jar a few days ago at about 10% colonization. Should be pretty much completely white when it's 100%, assuming that (wild guess) you're growing P. cubensis.
P. cubensis it is :-)
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: DetectiveLefty]
#21789536 - 06/10/15 06:00 PM (8 years, 7 months ago) |
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Quote:
DetectiveLefty said: Let it go a few more days- Ima say you shook that jar a few days ago at about 10% colonization. Should be pretty much completely white when it's 100%, assuming that (wild guess) you're growing P. cubensis.
Oats?!!! What are you, in Scotland?
Wrong. It has yeast infection. LC should colonize in 5-7 days. Two weeks is way too long.
OP, when you decide to toss the jar or spawn it, i promise a fine smokey looking powder will come out into the air
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21789589 - 06/10/15 06:13 PM (8 years, 7 months ago) |
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Quote:
azur said:
Quote:
DetectiveLefty said: Let it go a few more days- Ima say you shook that jar a few days ago at about 10% colonization. Should be pretty much completely white when it's 100%, assuming that (wild guess) you're growing P. cubensis.
Oats?!!! What are you, in Scotland?
Wrong. It has yeast infection. LC should colonize in 5-7 days. Two weeks is way too long.
OP, when you decide to toss the jar or spawn it, i promise a fine smokey looking powder will come out into the air
I hope thats not true.... I have just put 6 PF cakes made from the same LC to dunk, they smelled so fresh and nice and two of the jars had already made one and two side pins in the 1/2-pint jar.... On that background my thinking is that the LC must be pretty clean!?
It is hard to take a god picture, but I think that in this one taken in the closet where the jar lives, you can see some rhizomorph mycelium in the top....
 I am not trying to argue here, if it is yeast then it is yeast, but knowing that your judgment can only be as god as is my pictures I would like to ask if you still think it is yeast?
Edited by Peaceful Soul (06/11/15 04:28 PM)
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MudaFuka
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21793732 - 06/11/15 04:30 PM (8 years, 7 months ago) |
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Doesn't really look like yeast to me. You could probably user it now but I would give it a day or 3.
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
#21793759 - 06/11/15 04:36 PM (8 years, 7 months ago) |
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Quote:
MudaFuka said: Doesn't really look like yeast to me. You could probably user it now but I would give it a day or 3.
Honestly i can't say for sure if it looks like yeast. Upon zooming in the pic loses focus. But i can say that a 2 week old quart jar that was fucked with an LC should be densely colonized, loaded with bacteria, or nicely colonized with mold. The only other option is yeast. If that jar was 4 or 5 days old it would look normal. Again, pictures hide shit. Maybe it's fine. My experience tells me otherwise.
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
#21793773 - 06/11/15 04:39 PM (8 years, 7 months ago) |
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Thanks man 
Especialy for thisQuote:
MudaFuka said:I would give it a day or 3.
I now know what to do; 3 days more it shall be given
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21793781 - 06/11/15 04:41 PM (8 years, 7 months ago) |
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I still think the first pic looks bad. Use it if you want, but if it contaminates I'd blame the jars first picture. I'd also think about doing agar > lc next time
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21793802 - 06/11/15 04:46 PM (8 years, 7 months ago) |
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I just saw the pic in the other post. Who knows. I will say I've never used oats. Could just be some wimpy myc
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21793832 - 06/11/15 04:52 PM (8 years, 7 months ago) |
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I agree it's pretty wispy and there's lots of wet looking grains. Oats for me looks very different.

I agree with someone earlier. Animal feed like bird seed and oats needs a MINIMUM 2 hour pressure cook cycle for the bacterial endospores.
Edited by Mad Season (06/11/15 04:52 PM)
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21793914 - 06/11/15 05:05 PM (8 years, 7 months ago) |
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Quote:
azur said: I just saw the pic in the other post. Who knows. I will say I've never used oats. Could just be some wimpy myc
I guess only time can tell, and it will tell.... I have 8 more jars just like that and I am not gonna toss em
By the way; Half of the jars are inoculated with agar, they were a little behind to start with do to only one inoculation point (I think).
The reason for also using LC, given the fact that I had agar is that at the time I started Isolating on agar I did not know that I should have starte with a clone. My isolating was started from my MS LC. At the T2 state I was taught that my isolating would be in great risk of ending op with a poor fruiting strain, så I stopped my agar project immediately and inoculated half of the jars with the T2 agar and to widen the genetic pool I used the LC for the rest of my jars.
Quote:
Mad Season said: I'd also think about doing agar > lc next time
Thats allso my thinking, or maybe just Agar => grain
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21793930 - 06/11/15 05:09 PM (8 years, 7 months ago) |
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Quote:
peacful soul Thats allso my thinking, or maybe just Agar => grain

agar>grain>g2g
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21793951 - 06/11/15 05:12 PM (8 years, 7 months ago) |
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I'd much rather do lcs over g2g. At least you can test how clean lcs are on agar plates. It's a lot easier to fuck up a g2g. Speaking from experience. So tired of them hidden contaminations.
Even having a smallish sab fucks g2gs. Lots of us had to get new boxes for g2gs because the jars move air around so much
Edited by Mad Season (06/11/15 05:13 PM)
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21793963 - 06/11/15 05:14 PM (8 years, 7 months ago) |
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LC ftw
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21793982 - 06/11/15 05:16 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: I'd much rather do lcs over g2g. At least you can test how clean lcs are on agar plates. It's a lot easier to fuck up a g2g. Speaking from experience. So tired of them hidden contaminations.
Even having a smallish sab fucks g2gs. Lots of us had to get new boxes for g2gs.
or you can just use agar to see how clean ANY specimen is and clone and isolate. and i did my g2gs in open air with nothing but the utmost success.
i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC. especially if youre gonna use agar anyway why bother with LC? just use spores on agar>grain>g2g.
rather than ports etc and syringes all the time. seems like a no brainer 
to each their own i suppose.
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794019 - 06/11/15 05:25 PM (8 years, 7 months ago) |
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Quote:
i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC. especially if youre gonna use agar anyway why bother with LC? just use spores on agar>grain>g2g.
rather than ports etc and syringes all the time. see
You've obviously never used lc to its full potential. If you ever do, you'll be saying:
i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC. especially if youre gonna use agar anyway why bother with g2g?
LC/LI is the biggest time saver in this game. I can knock up jars with lc in circles around you doing g2g
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794033 - 06/11/15 05:27 PM (8 years, 7 months ago) |
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Quote:
twistedty said: or you can just use agar to see how clean ANY specimen is and clone and isolate. and i did my g2gs in open air with nothing but the utmost success. how do you test a whole grain jar with agar? You can test single grains but not the whole jar. A well shook lc put on a few dishes will tell you how clean it is and it's like a few extra days for a MASSIVE amount of clean inoculant. Also if you keep going in open air your luck will run out indefinitely.
i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC. especially if youre gonna use agar anyway why bother with LC? just use spores on agar>grain>g2g. uhh for lots of clean inoculant you can store easily? Honey and water... so hard..
rather than ports etc and syringes all the time. seems like a no brainer  with agar skills why would I need ports? I practiced agar to get sterile technique
to each their own i suppose. I haven't had the need for lcs since I've been focusing on agar. I'm saying that lcs in my opinion is far superior to g2gs. I'd rather have lots of inoculant I know is 100% clean.
Edited by Mad Season (06/11/15 05:33 PM)
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PinPornProducer
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21794054 - 06/11/15 05:32 PM (8 years, 7 months ago) |
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Looks like the op has a big temp difference issue, that's a lot of sweat in that jar
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21794067 - 06/11/15 05:34 PM (8 years, 7 months ago) |
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i have done LC and it was successful.
just saying when i was dropping in the wedge into the solution i was like why would i do this, why not just drop into a master jar.
and im pretty sure the 2 masters i made with the other wedges colonized faster than the LC after it was colonized.
i used to do 100 jar batches= 20 tubs. 20 jars a day roughly with 2 pressure cookers each holding 10 1qt. jars. using 20 masters nocced up with wedges from my slant library.

1 master jar made 5 g2g jars for uber colonization times.
imo way easier just using grains and agar. rather than syringes and syrupy LC concocitons with magnetic stirring blah blah blah.
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794090 - 06/11/15 05:40 PM (8 years, 7 months ago) |
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Quote:
twistedty said:blah blah blah.
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21794102 - 06/11/15 05:42 PM (8 years, 7 months ago) |
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Quote:
azur said:
Quote:
twistedty said:blah blah blah.
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794114 - 06/11/15 05:44 PM (8 years, 7 months ago) |
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Ha ha.
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21794123 - 06/11/15 05:47 PM (8 years, 7 months ago) |
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as i sad to each his own!
once you get a nice clean way of doing things with consistent success i guess it doesnt matter how you get there.
the key is CONSISTENT SUCCESS.
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MudaFuka
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794154 - 06/11/15 05:54 PM (8 years, 7 months ago) |
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Liquids are the shit. I can't imagine going back to agar wedges.
I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794162 - 06/11/15 05:57 PM (8 years, 7 months ago) |
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If you want 100% success the only way to do it is to start with no contaminations at all. Personally transferring endospore grains to more grains sounds silly to me, and you can't even tell if it's clean. I don't want any of my batches getting fucked so I don't mess with g2gs because it's fucked lots of my batches. Too risky. Like I said I trust tested lcs more than g2gs. I actually only do a2g as of late, but I'm more than down for some lcs now that I have clones again.
Yeah Muda wedges is so tedious.
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
#21794179 - 06/11/15 06:01 PM (8 years, 7 months ago) |
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Quote:
Mad Season said: If you want 100% success the only way to do it is to start with no contaminations at all. Personally transferring endospore grains to more grains sounds silly to me, and you can't even tell if it's clean. I don't want any of my batches getting fucked so I don't mess with g2gs because it's fucked lots of my batches. Too risky. Like I said I trust tested lcs more than g2gs. I actually only do a2g as of late, but I'm more than down for some lcs now that I have clones again.
Yeah Muda wedges is so tedious.
i dont understand how its to risky. if there is endospores in your grains to begin with you arent sterilizing properly and no matter what you use to nocc em up with it will still be a fail?
and you can never tell if your grain is clean in the first place? unless youre gonna put it on agar too?
i doubt g2g fucked up your batches just sayin
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
#21794192 - 06/11/15 06:03 PM (8 years, 7 months ago) |
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Quote:
MudaFuka said: Liquids are the shit. I can't imagine going back to agar wedges.
I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.
so you dont use agar wedges in your LC?
you should at least use agar for some aspects in this hobby.
i would be kicking myself if i didnt have it on some of the potent beasts i cloned/isolated and grew out.

sucker was 180g wet
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MudaFuka
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794205 - 06/11/15 06:07 PM (8 years, 7 months ago) |
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Agar is the cornerstone of everything I do. I make 25 plates a week minimum. I just don't inoculate grain with them.
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
#21794210 - 06/11/15 06:07 PM (8 years, 7 months ago) |
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Quote:
MudaFuka said: Agar is the cornerstone of everything I do. I make 25 plates a week minimum. I just don't inoculate grain with them.
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794237 - 06/11/15 06:12 PM (8 years, 7 months ago) |
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Quote:
twistedty said:
Quote:
MudaFuka said: Liquids are the shit. I can't imagine going back to agar wedges.
I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.
so you dont use agar wedges in your LC?
you should at least use agar for some aspects in this hobby.
i would be kicking myself if i didnt have it on some of the potent beasts i cloned/isolated and grew out.

sucker was 180g wet
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21794248 - 06/11/15 06:14 PM (8 years, 7 months ago) |
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thats some FATTTY PE SON dammmn.
i derailed this thread a bit :/
+5
Edited by twistedty (06/11/15 06:14 PM)
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Mad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794284 - 06/11/15 06:21 PM (8 years, 7 months ago) |
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Lol me too. You're probably right. It is something else but I still ran 16 a2gs and 16 g2gs originating from 16 clean a2gs at the same time and all 16 a2gs passed, with 15/16 g2gs contaminating.
I could probably do a better job now though since I'm guessing it's cause of my old small sab, but I still had better success with agar, and feel I'd have just as much success with lcs knowing it can be tested 5 days before making a bunch of jars. I couldn't imagine opening a grain jar 5 days before g2ging to test it on agar. It'd be a lot less sterile that way. Syringes seem cleaner to me because it's not being opened when testing a few days before using it.
I also pc for 2.5-3 hours so I doubt I sterilized badly doubling the standard time..
Edited by Mad Season (06/11/15 06:25 PM)
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maddchef
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
#21794293 - 06/11/15 06:23 PM (8 years, 7 months ago) |
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Quote:
twistedty said:
i dont understand how its to risky. if there is endospores in your grains to begin with you arent sterilizing properly
Lol @ sterilizing properly. You will always have endospores. Go make a jar on the wet side and sterilize it for 4 hours. Betcha get bacteria all in it.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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twistedty
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Re: Oat grain - is this jar ready to spawn? [Re: maddchef]
#21794303 - 06/11/15 06:25 PM (8 years, 7 months ago) |
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Quote:
maddchef said:
Quote:
twistedty said:
i dont understand how its to risky. if there is endospores in your grains to begin with you arent sterilizing properly
Lol @ sterilizing properly. You will always have endospores. Go make a jar on the wet side and sterilize it for 4 hours. Betcha get bacteria all in it.
agreed.
dryer is better than wetter any day of the week for sure
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azur
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21796275 - 06/12/15 07:38 AM (8 years, 7 months ago) |
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OP, Just some pics.
 First pic. One on left has yeast. One on right is healthy myc. Second pic is close up of yeast. Notice the yellow spots. Hope this helps
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Peaceful Soul
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
#21800909 - 06/13/15 06:15 AM (8 years, 7 months ago) |
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Quote:
azur said: OP, Just some pics.
 First pic. One on left has yeast. One on right is healthy myc. Second pic is close up of yeast. Notice the yellow spots. Hope this helps
I´m not sure exactly what to look fore, the myecilium(or yeast) looks pretty simpler in my eyes, they are however not as trained yet as is yours.
In the jar on the left picture and in the close up picture, I can see that som of the grains are not fully covert with mycelium, and grains are yellow so in that way I do see some yellow spots.... If that is not what you mean, then I am sorry to tell that I can not see any yellow spots
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Peaceful Soul
Noobie



Registered: 04/25/15
Posts: 70
Loc: EU
Last seen: 1 year, 5 months
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Re: Oat grain - is this jar ready to spawn? [Re: Peaceful Soul]
#21816636 - 06/16/15 08:54 PM (8 years, 7 months ago) |
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Update:
I spawned the jars today, nothing bad to report so far :-) The spawns further course can be followed here
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