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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
    #21793982 - 06/11/15 05:16 PM (8 years, 7 months ago)

Quote:

Mad Season said:
I'd much rather do lcs over g2g. At least you can test how clean lcs are on agar plates. It's a lot easier to fuck up a g2g. Speaking from experience. So tired of them hidden contaminations.

Even having a smallish sab fucks g2gs. Lots of us had to get new boxes for g2gs.





or you can just use agar to see how clean ANY specimen is and clone and isolate.  and i did my g2gs in open air with nothing but the utmost success. 

i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC.  especially if youre gonna use agar anyway why bother with LC?  just use spores on agar>grain>g2g.

rather than ports etc and syringes all the time. seems like a no brainer :shrug:

to each their own i suppose.


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Invisibleazur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794019 - 06/11/15 05:25 PM (8 years, 7 months ago)

Quote:

i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC.  especially if youre gonna use agar anyway why bother with LC?  just use spores on agar>grain>g2g.

rather than ports etc and syringes all the time. see



You've obviously never used lc to its full potential. If you ever do, you'll be saying:

i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC.  especially if youre gonna use agar anyway why bother with g2g? 

LC/LI is the biggest time saver in this game. I can knock up jars with lc in circles around you doing g2g


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InvisibleMad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794033 - 06/11/15 05:27 PM (8 years, 7 months ago)

Quote:

twistedty said:
or you can just use agar to see how clean ANY specimen is and clone and isolate.  and i did my g2gs in open air with nothing but the utmost success. 
how do you test a whole grain jar with agar? You can test single grains but not the whole jar. A well shook lc put on a few dishes will tell you how clean it is and it's like a few extra days for a MASSIVE amount of clean inoculant. Also if you keep going in open air your luck will run out indefinitely.

i mean so much extra time, work, hassle, equimpent, and sticky solutions with LC.  especially if youre gonna use agar anyway why bother with LC?  just use spores on agar>grain>g2g.
uhh for lots of clean inoculant you can store easily? Honey and water... so hard..

rather than ports etc and syringes all the time. seems like a no brainer :shrug:
with agar skills why would I need ports? I practiced agar to get sterile technique

to each their own i suppose.
I haven't had the need for lcs since I've been focusing on agar. I'm saying that lcs in my opinion is far superior to g2gs. I'd rather have lots of inoculant I know is 100% clean.




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Edited by Mad Season (06/11/15 05:33 PM)


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OfflinePinPornProducer
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
    #21794054 - 06/11/15 05:32 PM (8 years, 7 months ago)

Looks like the op has a big temp difference issue, that's a lot of sweat in that jar


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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
    #21794067 - 06/11/15 05:34 PM (8 years, 7 months ago)

i have done LC and it was successful.

just saying when i was dropping in the wedge into the solution i was like why would i do this, why not just drop into a master jar.

and im pretty sure the 2 masters i made with the other wedges colonized faster than the LC after it was colonized.

i used to do 100 jar batches= 20 tubs.  20 jars a day roughly with 2 pressure cookers each holding 10 1qt. jars.  using 20 masters nocced up with wedges from my slant library.



1 master jar made 5 g2g jars for uber colonization times.

imo way easier just using grains and agar.  rather than syringes and syrupy LC concocitons with magnetic stirring blah blah blah.




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Invisibleazur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794090 - 06/11/15 05:40 PM (8 years, 7 months ago)

Quote:

twistedty said:blah blah blah.




--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
    #21794102 - 06/11/15 05:42 PM (8 years, 7 months ago)

Quote:

azur said:
Quote:

twistedty said:blah blah blah.








:cheers:


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Invisibleazur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794114 - 06/11/15 05:44 PM (8 years, 7 months ago)

Ha ha.



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FALL IN LOVE WITH LC
FOTTSE!!!
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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
    #21794123 - 06/11/15 05:47 PM (8 years, 7 months ago)

as i sad to each his own!

once you get a nice clean way of doing things with consistent success i guess it doesnt matter how you get there.

the key is CONSISTENT SUCCESS.


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InvisibleMudaFuka
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794154 - 06/11/15 05:54 PM (8 years, 7 months ago)

Liquids are the shit. I can't imagine going back to agar wedges.

I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.


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InvisibleMad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794162 - 06/11/15 05:57 PM (8 years, 7 months ago)

If you want 100% success the only way to do it is to start with no contaminations at all. Personally transferring endospore grains to more grains sounds silly to me, and you can't even tell if it's clean. I don't want any of my batches getting fucked so I don't mess with g2gs because it's fucked lots of my batches. Too risky. Like I said I trust tested lcs more than g2gs. I actually only do a2g as of late, but I'm more than down for some lcs now that I have clones again.

Yeah Muda wedges is so tedious.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: Mad Season]
    #21794179 - 06/11/15 06:01 PM (8 years, 7 months ago)

Quote:

Mad Season said:
If you want 100% success the only way to do it is to start with no contaminations at all. Personally transferring endospore grains to more grains sounds silly to me, and you can't even tell if it's clean. I don't want any of my batches getting fucked so I don't mess with g2gs because it's fucked lots of my batches. Too risky. Like I said I trust tested lcs more than g2gs. I actually only do a2g as of late, but I'm more than down for some lcs now that I have clones again.

Yeah Muda wedges is so tedious.




i dont understand how its to risky.  if there is endospores in your grains to begin with you arent sterilizing properly and no matter what you use to nocc em up with it will still be a fail?

and you can never tell if your grain is clean in the first place?  unless youre gonna put it on agar too?

i doubt g2g fucked up your batches just sayin


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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
    #21794192 - 06/11/15 06:03 PM (8 years, 7 months ago)

Quote:

MudaFuka said:
Liquids are the shit. I can't imagine going back to agar wedges.

I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.




so you dont use agar wedges in your LC? 

you should at least use agar for some aspects in this hobby.

i would be kicking myself if i didnt have it on some of the potent beasts i cloned/isolated and grew out.



sucker was 180g wet


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InvisibleMudaFuka
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794205 - 06/11/15 06:07 PM (8 years, 7 months ago)

Agar is the cornerstone of everything I do. I make 25 plates a week minimum. I just don't inoculate grain with them.


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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: MudaFuka]
    #21794210 - 06/11/15 06:07 PM (8 years, 7 months ago)

Quote:

MudaFuka said:
Agar is the cornerstone of everything I do. I make 25 plates a week minimum. I just don't inoculate grain with them.




:nodofunderstanding:


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Invisibleazur
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794237 - 06/11/15 06:12 PM (8 years, 7 months ago)

Quote:

twistedty said:
Quote:

MudaFuka said:
Liquids are the shit. I can't imagine going back to agar wedges.

I've had oat jars colonise with thick white myc but I've also had some that look quite wispy like OPS. Some cultures just do that on oats. I've had kick ass grows started with jars that looked like that.




so you dont use agar wedges in your LC? 

you should at least use agar for some aspects in this hobby.

i would be kicking myself if i didnt have it on some of the potent beasts i cloned/isolated and grew out.



sucker was 180g wet





--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: azur]
    #21794248 - 06/11/15 06:14 PM (8 years, 7 months ago)

thats some FATTTY PE SON dammmn.


i derailed this thread a bit :/

+5


Edited by twistedty (06/11/15 06:14 PM)


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InvisibleMad Season
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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794284 - 06/11/15 06:21 PM (8 years, 7 months ago)

Lol me too. You're probably right. It is something else but I still ran 16 a2gs and 16 g2gs originating from 16 clean a2gs at the same time and all 16 a2gs passed, with 15/16 g2gs contaminating.

I could probably do a better job now though since I'm guessing it's cause of my old small sab, but I still had better success with agar, and feel I'd have just as much success with lcs knowing it can be tested 5 days before making a bunch of jars. I couldn't imagine opening a grain jar 5 days before g2ging to test it on agar. It'd be a lot less sterile that way. Syringes seem cleaner to me because it's not being opened when testing a few days before using it.

I also pc for 2.5-3 hours so I doubt I sterilized badly doubling the standard time..


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/11/15 06:25 PM)


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Re: Oat grain - is this jar ready to spawn? [Re: twistedty]
    #21794293 - 06/11/15 06:23 PM (8 years, 7 months ago)

Quote:

twistedty said:

i dont understand how its to risky.  if there is endospores in your grains to begin with you arent sterilizing properly





Lol @ sterilizing properly. You will always have endospores. Go make a jar on the wet side and sterilize it for 4 hours. Betcha get bacteria all in it.


--------------------
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Offlinetwistedty
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Re: Oat grain - is this jar ready to spawn? [Re: maddchef]
    #21794303 - 06/11/15 06:25 PM (8 years, 7 months ago)

Quote:

maddchef said:
Quote:

twistedty said:

i dont understand how its to risky.  if there is endospores in your grains to begin with you arent sterilizing properly





Lol @ sterilizing properly. You will always have endospores. Go make a jar on the wet side and sterilize it for 4 hours. Betcha get bacteria all in it.





agreed.

dryer is better than wetter any day of the week for sure


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