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sauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
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No mycelium, no signs of contamination, WTF
#21788553 - 06/10/15 02:13 PM (8 years, 7 months ago) |
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After first successful cubensis growing several months later it was attempted to start new PF cakes. But this time something happened and I hope somebody may know what. Standart recipe was used and spore print was working, for exception only different vendor vermiculite and added little chalk powder. Rising to 28-29C and adding more water didn't help. More than several weeks passed. Tried opening lid and probing to see if there was any contamination - none. Strangely unlike previously substrate was easy to keep sterile. Was someting in vermiculite causing that or spores somehow were killed?
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna
Edited by sauroman1 (06/10/15 02:17 PM)
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: No mycelium, no signs of contamination, WTF [Re: sauroman1]
#21788600 - 06/10/15 02:24 PM (8 years, 7 months ago) |
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Did you scrape a spore print onto your verm layer?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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nwalker248
Solo Trekker



Registered: 08/22/14
Posts: 396
Last seen: 3 years, 8 months
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Re: No mycelium, no signs of contamination, WTF [Re: maddchef]
#21788795 - 06/10/15 03:12 PM (8 years, 7 months ago) |
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How much air flow do they have? Method of inoculation? Definitely see liquid running down the side if using just a syringe?
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sauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
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Re: No mycelium, no signs of contamination, WTF [Re: nwalker248]
#21800525 - 06/13/15 02:17 AM (8 years, 7 months ago) |
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Sorry, forgot to mention inoculation technique. Syringe with sterile water and spore mix was injected into pf cake. Restarted one jar this time soaking verm in water and not adding chalk in case there was some fungicides. Also UV (UV-B) lamp was used to sterilize air, rays might have hit spores too but it was 3 meter distance and for not a lot of seconds.
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna
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ShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Re: No mycelium, no signs of contamination, WTF [Re: sauroman1]
#21800538 - 06/13/15 02:24 AM (8 years, 7 months ago) |
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MS is inherently variable. Try again
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sauroman1
Emrys

Registered: 03/22/14
Posts: 203
Loc: Shangrila
Last seen: 1 month, 9 days
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Re: No mycelium, no signs of contamination, WTF [Re: ShroominMe]
#21804377 - 06/14/15 12:10 AM (8 years, 7 months ago) |
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MS stands for?
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.” ― Terence McKenna
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BkillsU
Stranger



Registered: 05/10/15
Posts: 58
Last seen: 2 years, 8 months
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Re: No mycelium, no signs of contamination, WTF [Re: sauroman1]
#21804399 - 06/14/15 12:16 AM (8 years, 7 months ago) |
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MS means use the search function
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lusus
Stranger

Registered: 04/06/10
Posts: 229
Loc: EUROPA
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Re: No mycelium, no signs of contamination, WTF [Re: BkillsU]
#21804539 - 06/14/15 12:57 AM (8 years, 7 months ago) |
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ms= multispore
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