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Cessna172r
Stranger Danger



Registered: 06/23/14
Posts: 13
Last seen: 3 years, 11 months
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Agar: Contam or Healthy Mycelium?
#21787864 - 06/10/15 11:07 AM (8 years, 7 months ago) |
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Hey guys!
I completed my first pf grow last year to great success, so i decided to try my hand at grain. But first, I wanted to mess with some agar. Initially I had some trouble with contams, but after cleaning up my technique I feel I have gotten some results, although I am not 100% sure its what im looking for. Using a premixed MEA powder, I made several plates and inoculated 8 of them with a LC that has been sitting on a shelf for month or two. After about 2 days, I noticed a small speck on several of the plates which branched out into a filamentous/filiform pattern covering a sizable section of the plates. I let the growth continue for a week and a half waiting for it to turn green but as of right now, all the plates have white fluffy rhizomorphic growth. I used the same culture to noc up some wbs and ended up with a spot of green mold and also what looks to be cube mycelium. I am still nervous that it could be some kind of white mold so I decided to ask the experts here at the contam board before I proceed. A theory I have is that it is cube mycelium, but my agar is too nutritious, eliminating the need for the mycelium to grow aggressively. Sorry for the less than stellar pics/condensation, couldn't find the camera charger. If more/better pics are needed, let me know. Thanks in advance for your help! 


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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Agar: Contam or Healthy Mycelium? [Re: Cessna172r]
#21787875 - 06/10/15 11:10 AM (8 years, 7 months ago) |
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These look ok to me, other than the multiple sites of colonization, how much spore solution did you use?
-------------------- ¯\_(ツ)_/¯
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar: Contam or Healthy Mycelium? [Re: Tmethyl]
#21787890 - 06/10/15 11:17 AM (8 years, 7 months ago) |
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it's legit af to see you tmethyl! Wondered where you were. When I was here rockin it 2 or 3 years ago I saw you all over the place.
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Cessna172r
Stranger Danger



Registered: 06/23/14
Posts: 13
Last seen: 3 years, 11 months
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Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
#21787909 - 06/10/15 11:23 AM (8 years, 7 months ago) |
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They were inoculated from a single drop of LC I made myself, which may have spread around a little bit.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar: Contam or Healthy Mycelium? [Re: Cessna172r]
#21787919 - 06/10/15 11:26 AM (8 years, 7 months ago) |
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That's good. I like to let it swirl around the plate to show off any contamination. That way if there is a contamination, it wouldn't be blocked by mycelium sitting on a single drop.
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Cessna172r
Stranger Danger



Registered: 06/23/14
Posts: 13
Last seen: 3 years, 11 months
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Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
#21787997 - 06/10/15 11:53 AM (8 years, 7 months ago) |
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Awesome! I'm quite pleased that my sterile technique appears to be on point. Think im gonna try and isolate now. Thanks for the input guys
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
#21788805 - 06/10/15 03:15 PM (8 years, 7 months ago) |
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Quote:
Mad Season said:
it's legit af to see you tmethyl! Wondered where you were. When I was here rockin it 2 or 3 years ago I saw you all over the place.
I remember Mad Season  Good to see you too my friend, I try to be as frequent as possible but mostly find myself in the Mushroom Identification forum as I'm a Trusted Identifier.
-------------------- ¯\_(ツ)_/¯
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