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OfflineCessna172r
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Registered: 06/23/14
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Agar: Contam or Healthy Mycelium?
    #21787864 - 06/10/15 11:07 AM (8 years, 7 months ago)

Hey guys!

I completed my first pf grow last year to great success, so i decided to try my hand at grain. But first, I wanted to mess with some agar. Initially I had some trouble with contams, but after cleaning up my technique I feel I have gotten some results, although I am not 100% sure its what im looking for.
Using a premixed MEA powder, I made several plates and inoculated 8 of them with a LC that has been sitting on a shelf for month or two. After about 2 days, I noticed a small speck on several of the plates which branched out into a filamentous/filiform pattern covering a sizable section of the plates. I let the growth continue for a week and a half waiting for it to turn green but as of right now, all the plates have white fluffy rhizomorphic growth. I used the same culture to noc up some wbs and ended up with a spot of green mold and also what looks to be cube mycelium. I am still nervous that it could be some kind of white mold so I decided to ask the experts here at the contam board before I proceed. A theory I have is that it is cube mycelium, but my agar is too nutritious, eliminating the need for the mycelium to grow aggressively. Sorry for the less than stellar pics/condensation, couldn't find the camera charger. If more/better pics are needed, let me know. Thanks in advance for your help! :grin:







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OfflineTmethylM
Smear in the shale
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Registered: 07/16/12
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Re: Agar: Contam or Healthy Mycelium? [Re: Cessna172r]
    #21787875 - 06/10/15 11:10 AM (8 years, 7 months ago)

These look ok to me, other than the multiple sites of colonization, how much spore solution did you use?


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
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Re: Agar: Contam or Healthy Mycelium? [Re: Tmethyl]
    #21787890 - 06/10/15 11:17 AM (8 years, 7 months ago)

:whathesaid: it's legit af to see you tmethyl! Wondered where you were. When I was here rockin it 2 or 3 years ago I saw you all over the place.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineCessna172r
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Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
    #21787909 - 06/10/15 11:23 AM (8 years, 7 months ago)

They were inoculated from a single drop of LC I made myself, which may have spread around a little bit.


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InvisibleMad Season
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Re: Agar: Contam or Healthy Mycelium? [Re: Cessna172r]
    #21787919 - 06/10/15 11:26 AM (8 years, 7 months ago)

That's good. I like to let it swirl around the plate to show off any contamination. That way if there is a contamination, it wouldn't be blocked by mycelium sitting on a single drop.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineCessna172r
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Registered: 06/23/14
Posts: 13
Last seen: 3 years, 11 months
Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
    #21787997 - 06/10/15 11:53 AM (8 years, 7 months ago)

Awesome! I'm quite pleased that my sterile technique appears to be on point. Think im gonna try and isolate now. Thanks for the input guys :thumbup:


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OfflineTmethylM
Smear in the shale
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Posts: 16,431
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Re: Agar: Contam or Healthy Mycelium? [Re: Mad Season]
    #21788805 - 06/10/15 03:15 PM (8 years, 7 months ago)

Quote:

Mad Season said:
:whathesaid: it's legit af to see you tmethyl! Wondered where you were. When I was here rockin it 2 or 3 years ago I saw you all over the place.



I remember Mad Season :awesomenod:
Good to see you too my friend, I try to be as frequent as possible but mostly find myself in the Mushroom Identification forum as I'm a Trusted Identifier.


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¯\_(ツ)_/¯


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