Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures, Substrate Bags   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Two year old spore swab: Whats my best option for revival?
    #21784339 - 06/09/15 05:39 PM (8 years, 7 months ago)

I've only got one chance, two spore laden swabs in a single sterile package.

I was planning to go straight to agar with it, but now I'm thinking letting them soak in water for a day or two would probably have a higher chance of success.

Whatchya think, is a making a spore syringe my best option?


Extras: Filter Print Post Top
OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
Re: Two year old spore swab: Whats my best option for revival? [Re: Psilosopherr]
    #21784424 - 06/09/15 05:57 PM (8 years, 7 months ago)

You can go whichever route you want, but I would personally just make a batch of agar on the wet side and inoculate a bunch of plates and wait. You can hydrate those spores via syringe, but ultimately they'll end up on agar anyway so making it on the moist side just negates the syringe step in my opinion.


--------------------


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Two year old spore swab: Whats my best option for revival? [Re: Psilosopherr]
    #21784430 - 06/09/15 05:58 PM (8 years, 7 months ago)

Make a syringe with one agar with the other. More wet agar helps too or less stiff whichever way you look at it


Extras: Filter Print Post Top
OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
Re: Two year old spore swab: Whats my best option for revival? [Re: bodhisatta]
    #21784447 - 06/09/15 06:02 PM (8 years, 7 months ago)

Quote:

bodhisatta said:
Make a syringe with one agar with the other. More wet agar helps too or less stiff whichever way you look at it




I should have just said higher water to agar ratio or something. "Moist" just popped in my head for some reason. :sexysmile:


--------------------


Edited by TheMustardTiger (06/09/15 06:10 PM)


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Two year old spore swab: Whats my best option for revival? [Re: TheMustardTiger]
    #21784470 - 06/09/15 06:06 PM (8 years, 7 months ago)

thanks to the both of you. I wouldn't have thought to alter the agar mix.

Does it merely have a higher moisture content or does it alter the texture/consistency?


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Two year old spore swab: Whats my best option for revival? [Re: Psilosopherr]
    #21784497 - 06/09/15 06:12 PM (8 years, 7 months ago)

it's less stiff. with less agar or more water. 1.5-2% is the usual range. I would maybe try 13-15g/L


Extras: Filter Print Post Top
OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
Re: Two year old spore swab: Whats my best option for revival? [Re: Psilosopherr]
    #21784510 - 06/09/15 06:14 PM (8 years, 7 months ago)

Quote:

rbalzer said:
thanks to the both of you. I wouldn't have thought to alter the agar mix.

Does it merely have a higher moisture content or does it alter the texture/consistency?




If you bought commercially prepared agar, it will have the mixture ratio printed on the label which gives you an industry standard consistency of sorts. The amount of moisture noticable to you depends on your experience with agar and the amount of water you use. Obviously, too much water will be counterproductive and too little would be counter intuitive. Just use common sense/trial and error and you'll be fine. Use the search function and try to find some people who have done this. I'm sure they're out there.  :cool:


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures, Substrate Bags   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale


Similar ThreadsPosterViewsRepliesLast post
* best spore vendor?
( 1 2 3 all )
gray1 44,192 44 08/26/17 10:33 AM
by ThirdEyeHigh
* What to do with a spore swab? *DELETED* oscill8 1,153 1 10/10/01 09:36 PM
by dimitri211
* wasted spores Blu Spore 2,982 3 02/22/19 10:59 AM
by LosTresOjos
* Re: Spore Price Comparison spores 5,039 16 07/27/00 12:46 PM
by Mycelium5150
* Making Spore Syringes Anonymous 7,245 13 09/04/02 08:14 PM
by Sammy
* Spore Prints came out DARK .... badtz 2,348 16 04/07/03 04:03 PM
by SixTango
* about old kit's spores baronwastelands 741 4 06/19/02 07:12 AM
by baronwastelands
* How do YOU make your spore prints? tekramrepus 8,277 18 11/14/02 03:44 PM
by kb73

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
426 topic views. 37 members, 215 guests and 17 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.008 seconds on 14 queries.