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Offlinemagicallyfunky
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CONTAMINATION
    #21783058 - 06/09/15 12:30 PM (8 years, 7 months ago)
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OK... I started last week and I was unsure how to read the syringe, (found out later it was line per hole.  I checked my jars today as you will see there are 5 jars with pink in them, (these are the ones that I over colonized) 1 with green mold in them.  The others look ok so far.  Can someone tell me what the pink is? I know what the green is. Pisses me off.  Heeeeeeeeeeeeeeeelp...


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: magicallyfunky]
    #21783075 - 06/09/15 12:37 PM (8 years, 7 months ago)

I dont know why the damn video came out sideways. Anyway the first jar shown is green mold, the second jar is the one with the pink at the bottom, the last jar is a good jar.  The first two were over inoculated and I believe that is why they are a waste.  If anyone has any suggestions on how to save the pink ones please give me a heads up.  I know the green one is toast.  I'm also afraid I screwed up the other ones just at a slower pace.  This all happened in the first week after incoculation.  :/

Thanks,
Mag
(Guess I'm not so f'in MagicallyFunky after all cept for the Funk part).  Totally annoyed!:mad2:


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

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Offlinesirdabz710
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Re: CONTAMINATION [Re: magicallyfunky]
    #21783082 - 06/09/15 12:39 PM (8 years, 7 months ago)

pictures would be best


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OfflineDensePlacebo
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Re: CONTAMINATION [Re: sirdabz710]
    #21783116 - 06/09/15 12:48 PM (8 years, 7 months ago)

Video is of poor quality. I can't tell what the pink is. What I can tell you is there is no such thing as over inoculating. The more of the syringe you put it in there the faster it will colonize. Seems to me your sterile technique is to blame.


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Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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InvisibleMad Season
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Re: CONTAMINATION [Re: DensePlacebo]
    #21783181 - 06/09/15 01:07 PM (8 years, 7 months ago)

Fusarium is pink/purple and there's lots of documented cases of people getting infected with it and going to the hospital for weeks if not months. Anything pink/purple/black gets tossed outside ASAP.

It could be techniques or syringe. Adding lots  of solution may make it quicker, but it also adds a higher chance for contam spores from the syringe going into the jar. If you flame sterilized and have a dry verm layer, my money's on syringe if it hit that many jars. We do agar work to get around that.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: sirdabz710]
    #21783182 - 06/09/15 01:07 PM (8 years, 7 months ago)

I'll try again said it would not allow .jpg files.  Give me one sec. And of course I cant figuire it out.


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
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InvisibleMad Season
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Re: CONTAMINATION [Re: magicallyfunky]
    #21783191 - 06/09/15 01:10 PM (8 years, 7 months ago)

Dude anything pink should be tossed for your own health and safety (google fusarium). Mycelium isn't pink. It's white.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/09/15 01:16 PM)


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: Mad Season]
    #21783361 - 06/09/15 01:52 PM (8 years, 7 months ago)
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Pics, I hope...


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
Funkilicious


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InvisibleMad Season
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Re: CONTAMINATION [Re: magicallyfunky]
    #21783481 - 06/09/15 02:25 PM (8 years, 7 months ago)

Yes it worked and I'm still standing by what I say. I'd never use anything turning pink


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: Mad Season]
    #21784393 - 06/09/15 05:51 PM (8 years, 7 months ago)

I followed the video to a T. Including making an inoculation training her. I clams everything with alcohol before I inoculated.  I fired the needles between  each jar. I followed the instructions per the video. I think I'm doing pretty good to have 20 jars going right (knock on wood) and only five wrong.  We shall see at the end how good the other ones are going to turn out. Not dumping others until all are done. I just want to see what they do.


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
Funkilicious


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: magicallyfunky]
    #21787181 - 06/10/15 07:25 AM (8 years, 7 months ago)

The only thing I'm doing different is I'm using pint jars instead of 1/2 pint jars. I've seen a lot of healthy pint jars. I can't figure it out. I followed the video to a "T" cept jar size. Wish mycelium had the power to kick the light pink fungi ass! Someone told me the name of it. It sTarts with an f w an f. I was praying that since I have an  experienced shroomer guiding me through the process that my first attempt would be perfect. :confused:  Do any of you prefer The sterilization of the jars for 90 minutes or do you find more success in sterilizing the jars with a pressure cooker. I need help in figuring out what could have happened and how to avoid it and not someone just saying throw them out. I am here to learn and want to know why and how could it have been prevented what it's called and si in and so forth. Here are just a few question. Question when grinding your brown rice flour do you leave it course or more like a flour?  First batch that I made up with the help of my shroomer friend was course not floury. Then it was suggested that I toss is because I  could have screwed up the moisture in the BRF and verm because I did not have the lids on properly and re sterilized then,  just to double make sure that I did not have any contaminants.  I actually burnt up the grinder making the second BRF it was very fine just like real flour. So recap: question 1. what do you think could have gone wrong? Question number two should they brown rice flour be course or should it be fine like real flower? Question number three does anyone have a preference to bowling for 90 minutes or using a pressure cooker? Question number four anyone have any suggestions that are not in the video that would ensure that my brown rice flour and verm cakes are contaminate my jars? ie like should I bike the vermiculite on 350 for the recommended time to put over the cakes to prevent further contamination instead of waiting until the dunk and roll? Please any help that you could give or suggest would be greatly appreciated I want to learn and I'm willing to do anything all I have is time on my hands and I want to stay busy.  I could not get my pics to show in this forum but I posted them in my journal.


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
Funkilicious


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InvisibleMad Season
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Re: CONTAMINATION [Re: magicallyfunky]
    #21787213 - 06/10/15 07:42 AM (8 years, 7 months ago)

I think it's impossible to work on it all without starting clean from the root. Syringes are not clean. You can pull out all your hairs over this one but vendor syringes just aren't clean. I don't think you grasp just how many organisms live in the air all around us, and the moment a mushrooms veil goes down, its gills have all been exposed to that air.

On a different note though lol. You germinate on agar and get a clean healthy culture. You can make an LI or an LC out of it. After testing it on agar again to see if your syringe you made is indeed clean, inoculate your jars. At this point you have a clean inoculant. I always start at the inoculant when dealing with contamination. Start at the source of it all, then work on the next steps that follow suit. I find inoculation/under sterilizing jars though to always be the root of contams. I always throw things in the pc for 45 minutes with agar and 2 hours with cakes/grains. Hope this helps :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/10/15 07:47 AM)


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: Mad Season]
    #21788971 - 06/10/15 03:52 PM (8 years, 7 months ago)

Omg you just blew my mind. Lol. Keep in mind I'm a noob. I hate being called that but I am what I am. :confused:


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
Funkilicious


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Offlinemagicallyfunky
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Registered: 05/29/15
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Re: CONTAMINATION [Re: Mad Season]
    #21801625 - 06/13/15 10:19 AM (8 years, 7 months ago)

Can you explain what agar is? I'm new and I looked at it and it seems to be very complicated.  Dont you think that I should get this technique down first  before attempting to perfect another one?


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
Funkilicious


Edited by magicallyfunky (06/13/15 10:22 AM)


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InvisibleMad Season
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Re: CONTAMINATION [Re: magicallyfunky]
    #21802171 - 06/13/15 11:32 AM (8 years, 7 months ago)

What technique?.. you mean sterility? Yeah you have to get sterile techniques down and the only way to have a clean inoculant is with agar. It is the #1 way to work on sterile techniques. This is what every person starting out should do. Agars easy as balls. Easier than the pf tek. All you need is a still air box, agar agar, and dry light malt extract.

2 grams agar agar found in Asian markets and 2 grams light malt extract, found in brew stores for every 100 ml of water. That's my recipe. I pour into pp5s and pc them kinda like:

http://www.shroomery.org/forums/showflat.php/Number/19208976

Here's why everyone starting should do agar.

this is a plate with at least 6 different types of bacteria and the mycelium. You can see contaminants. Altho this one is too far gone to be saved, you can save lots of cultures.

For thi plate the yellow stuff around the edges is bacteria. I cut it out and got 100% clean mycelium:


This is the only way for you to get a clean culture from a syringe because it could start off dirty and you can still cut out a clean wedge. Also if you want to clone fruits you need this, and all you need is a couple drops of spore solution to make as many jars as you'd like. I mean thousands of jars.

I have over a hundred jars right now all from a few drops of spores. My syringe is still above the 10cc line.


Edited by Mad Season (06/13/15 11:51 AM)


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Offlinemagicallyfunky
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Re: CONTAMINATION [Re: magicallyfunky]
    #21805807 - 06/14/15 11:25 AM (8 years, 7 months ago)

I will do this next time.  I have 2 lizard kings and 1/2 Ratan Thailand left.  So I need peetre dishes, how many? and where do you get the solution? Urgh cant remember what you called the solution.  Is it a slower process? do you still use rice cakes? You opened up a can of curious.


--------------------
Jel-O
Everyone carries a piece of the puzzle. Nobody comes into your life by mere coincidence. Trust your instincts. Do the unexpected. Find the others!
- Timothy Leary

Magically Funkilicious
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InvisibleMad Season
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Re: CONTAMINATION [Re: magicallyfunky]
    #21805821 - 06/14/15 11:30 AM (8 years, 7 months ago)

Disposable petris are pretty cheap and a 1 time use.

http://www.shroomery.org/forums/showflat.php/Number/19208976

http://www.shroomery.org/forums/showflat.php/Number/20111637#20111637

http://www.shroomery.org/forums/showflat.php/Number/21032261#21032261

These use glad Tupperware containers(pp5s). I've done both and prefer pp5 containers.

My recipe is a malt extract agar recipe. Agar agar is like Asian jello. It turns water to gel. No brf or verm at all.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/14/15 11:33 AM)


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