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Offlinefreddybrek
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Registered: 06/09/15
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Last seen: 8 years, 7 months
Contam on agar or not?
    #21781888 - 06/09/15 06:39 AM (8 years, 7 months ago)

I have some petri dishes, using MEA with some myc from a spore print, I think this is the third transfer, anyway, I'm fairly new to agar and since I've had these I've noticed a few spots in the agar.


Bottom view


It looks to me like my malt extract hasn't dissolved properly in the agar, and has either caramelized, or dried out( I noticed the agar gets more and more yellow the drier it gets) and the spots haven't grown at all, but they have been kept in the fridge?


Top view

I was planning on making more transfers anyway, which means whether its a contaminant or not I can isolate away from it, but I was planning on making an lc with the current myc just so I could get some mushrooms in the mean time.

Also, yes I have noticed the bacteria[?] growing in between 1 and 2 o'clock on the 1st image, I have a bunch of dishes all from the same prints, they all have the brown clumps, so obviously this one wouldn't be the one I use for the lc, this was just the clearest for the pictures

thanks for taking the time to read through my ramblings, any insight would be great


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