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OfflineTheMattStallion
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Registered: 08/08/14
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Any ideas on wet looking rye and darker mycelium?
    #21780688 - 06/08/15 08:45 PM (8 years, 7 months ago)





These jars were made using soaked then boiled rye, pressure cooked then cooled then innoced in a sterile environment.

incubated at 77F for about 3 weeks now in a closet
The myco is darker like a light gray almost and some parts of the rye look wet, they were also shaken a few days ago, looked fine before then


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780710 - 06/08/15 08:51 PM (8 years, 7 months ago)

Looks bacterial, I just tossed a jar the other day that looked almost identical to that


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21780741 - 06/08/15 08:59 PM (8 years, 7 months ago)

dont tell me that man. Ive been trying to grow for a year always keeping on top of new teks and info and I keep my inoc environment more sterile than half the people i ask on here and i still havent had one fully successful batch. only gotten less than 70 g's wet since i started with cakes and everyone whom i explain my technique to cant understand why it doesnt work


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780783 - 06/08/15 09:08 PM (8 years, 7 months ago)

How long you pc for and at what psi?
What is your source of inoculation? Syringes can harbour bacteria and mold no matter how sterile you are, if the contents on the syringe is dirty then you will have issues almost every time. Do you use agar at all? If not I highly recommend it


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21780795 - 06/08/15 09:11 PM (8 years, 7 months ago)

pc at 15 psi for 1 hour, syringe from The Spore Store, if i cant manage any good grows on syringes like many use on here idk if I can make augur work, do you have a good link to a tek on it?


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780811 - 06/08/15 09:14 PM (8 years, 7 months ago)

Dude I shake them up when I'm not sure. If it recovers in 3 days I call it good. If not junk them. But I live in an area where its been hot I run my ac all day and it causes the same shit to happen to my jars.

All else fails crack the jar and take a wiff.
People may say it leaves like Seconds for contams to sneak in.
I just say gives me enough time to get a wiff.
I'm not saying crack all the jars either.
One jar, one sniff. One opinion.


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: Thoky.ykoht]
    #21780828 - 06/08/15 09:17 PM (8 years, 7 months ago)

also if its relevant i have foil over the jar lids because i had to use narrow mouth SFDs on wide mouth jars so i used it as a precaution, so no air exchange


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780840 - 06/08/15 09:20 PM (8 years, 7 months ago)

15 psi for a minimum of 90 min for any grains man.

Agar is easy as pie, I use THIS guys tek. Its simple and works great. You just squirt a drop from the syringe onto the agar and let it grow out a little, contams pop right out and all you do is cut out a section of clean growth and transfer it to a new plate and repeat the process until you have a clean culture. One plate can be cut into wedges and inoc 6 quart jars easily.
Here is a master plate I cut wedges out of to inoc new plates


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InvisibleMad Season
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780861 - 06/08/15 09:25 PM (8 years, 7 months ago)

Just punch a hole in the lid and cut a piece of sfd bigger than the hole and silicone it to the lid over the hole. That's how everyone does it. You wouldn't need foil.

I pc at 15 psi for 2 hours. Twice as long as you. I upped it from 90 minutes due to bacteria. Lastly I switched to agar for clean inoculation. You want to step up the game? Just do it right. I got tired of failures too. In hindsight I was just being stubborn and stupid.

90 minutes:


120 minutes:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/08/15 09:27 PM)


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21780876 - 06/08/15 09:29 PM (8 years, 7 months ago)

I kept getting bad syringes from the store on here
And when I got set up with agar I never looked back.
Here's my latest rye adventure / with agar
I sucked with agar at first then blam.
http://www.shroomery.org/forums/showflat.php/Number/21545546/fpart/all

I use jars with 1/2-1" hole in the lid stuffed very tightly


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OfflineMachiavelliavore
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780878 - 06/08/15 09:29 PM (8 years, 7 months ago)

Quote:

TheMattStallion said:
pc at 15 psi for 1 hour, syringe from The Spore Store, if i cant manage any good grows on syringes like many use on here idk if I can make augur work, do you have a good link to a tek on it?




a) 15 psi for 1hr is the bare minimum, and may not have been enough.  Typically it's 90 mins.  I have gotten away with 65 for WBS and Grass Seed, but they are smaller grains less prone to harboring bacteria.

b) If you did not allow your grains to soak 24hr, all bacterial endospores may not have germinated prior to the PC run, and could have survived.  Apparently Rye is especially prone to endospore issues due to its geometry and size.

c)  If you have a bacterially contaminated syringe you're kinda fucked no matter what unless you use agar or do the following:  If you don't want to work with agar, prepare a small quantity of grain, say 1/8th cup per jar in half pint jars, noc a few.  They should colonize quickly, and you will see any bacteria (looks like gooey yellowish syrup.)  Try to transfer a colonized grain from a healthy, uncontaminated area, to another 1/8th cup jar, look for contams again.  Once you have a jar fully colonized, transfer that to a half full quart jar of grain, shake, then G2G the rest of your grain jars when that one is done.  45m would probably be plenty to PC such small jars.

Also, you might want to let your non-inoculated grain jars sit for like 1-1.5 weeks and see if you start seeing the bacterial goo.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: Any ideas on wet looking rye and darker mycelium? [Re: Machiavelliavore]
    #21780890 - 06/08/15 09:32 PM (8 years, 7 months ago)

Soaking doesn't make all endospores magically disappear, and could even increase population. That's just misinformation. Pcing for 1.5-3.5 hours is what does it. If you get bacteria just up the pc times.


Quote:

bodhisatta said:



clostridum butyricum from brewers grain(rye malt) it's very heat resistant because of the spores. it is responsible for the most part in the rancid smell if you let grains soak too long. it will survive the boil and is why your grains go bad and smell like puke and Greek feet feta if you don't sterilize them after your grain prep.

it forms endospores even culture on anaerobic plates with an abidance of nutrients. as you can see some subset of the population finds it appropriate to form endospores despite not having "bad conditions" as conditions get worse spore formation goes up. I would be nearly positive you end up with more endospores if you do a soak than if you skip a soak, either way these DO get killed by a PC in a sufficient cycle.





--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (06/08/15 09:34 PM)


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: Mad Season]
    #21780919 - 06/08/15 09:40 PM (8 years, 7 months ago)

Im going to try agar but it seems so risky since the SAB requires such still air but having used it, it also requires a lot of movement to alcohol the jar, flame the syringe, and place your hands back in, then you have to wait around 30 for the air to settle leaving your syringe just sitting there in the open


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780938 - 06/08/15 09:44 PM (8 years, 7 months ago)

I use a sealed glove box with a port for sterilizing my scalpel. Lower right side of the box. I just keep the flame a few inches away and stick the scalpel out the hole into the flame between transfers.


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780939 - 06/08/15 09:44 PM (8 years, 7 months ago)

I cut two holes in a clear box
I get in I get out, stop over thinking it.


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780944 - 06/08/15 09:45 PM (8 years, 7 months ago)

Idk what to think about mushroom growing, Ive given it my best tons of times and each time it fails I get a bunch of "Obviously you shouldve done this instead of that" when I was following the instructions of people with many successful grows, so how does everyone else do it with the same techniques that Im told are my downfall?


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


Extras: Filter Print Post Top
OfflineTheMattStallion
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Registered: 08/08/14
Posts: 103
Last seen: 8 years, 5 months
Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780955 - 06/08/15 09:47 PM (8 years, 7 months ago)

See I was told by everyone Ive asked so far "never glove box, always SAB" and everyone also says "must disturb air as little as possible, must also perform 50 air disturbing actions with breaks inbetween"


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21780959 - 06/08/15 09:48 PM (8 years, 7 months ago)

If you start with bad syringes you get no where.
Nobody wants to sit here and beat up on the vendors though.


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: Thoky.ykoht]
    #21780973 - 06/08/15 09:50 PM (8 years, 7 months ago)

if the 20 or so syringes ive gotten from 5 or more sources going to contam my jars how will I get anywhere with agar?


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


Extras: Filter Print Post Top
OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21781007 - 06/08/15 09:57 PM (8 years, 7 months ago)

Agar is used to clean up dirty syringes. Trust me man, get use to agar and you will not regret it


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OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21781015 - 06/08/15 09:58 PM (8 years, 7 months ago)

If I didn't use agar I'd prolly be in the same boat you are


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InvisibleMad Season
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21781024 - 06/08/15 10:00 PM (8 years, 7 months ago)

You cut clean mycelium away from contamination and transfer to new dish.



the yellow around the edges is bacteria and I cut from where it is still clean. I transfered to a new dish and it's 100% clean with no bacteria.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineTheMattStallion
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Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21781029 - 06/08/15 10:01 PM (8 years, 7 months ago)

so whats your advice on the SAB? is widely viewed as the best tek for the job but it still seems too risky to take my arms out to flame the syringe or blade and then wait for 30 seconds while the air settles with my tool exposed.


--------------------
I want to be the very best.

Like no one ever was.

To grow them is my real test.

To eat them is my cause.

Pscylo-cy-bin!!!!


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OfflineMachiavelliavore
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Re: Any ideas on wet looking rye and darker mycelium? [Re: TheMattStallion]
    #21781079 - 06/08/15 10:23 PM (8 years, 7 months ago)

If you are using poly or silicon injection you don't need to use a SAB at all, it just helps.  You can flame sterilize in the SAB even though some might say that's making a mockery out of the still air.  It's still an airspace that has been flushed of contams by lysol, and is bound to be a lot cleaner than what's in your kitchen, on the off chance a contam lands on your needle after you flame it.
I flame in my SAB since I feel the ruckus of going in and out would cause more disruption than using a lighter, and disruption with the outside air, not the cleaner interior air.

Chances are, your contam source is the grain itself or your spore syringe.

Again, I recommend making some 1/8c grain test dishes.  Spore solution is a pretty poor inoculation method for a quantity of grain as large as a quard jar in my opinion, and works much better on PF size or smaller quantities of grain.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePinPornProducer
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Re: Any ideas on wet looking rye and darker mycelium? [Re: PinPornProducer]
    #21781179 - 06/08/15 10:50 PM (8 years, 7 months ago)

Quote:

PinPornProducer said:
I use a sealed glove box with a port for sterilizing my scalpel. Lower right side of the box. I just keep the flame a few inches away and stick the scalpel out the hole into the flame between transfers.





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